Mucosal Vaccination of Fish

Rombout, J.H.W.M.; Kiron, Viswanath

doi:10.1002/9781118806913.ch6

Cited by 25 publications

(18 citation statements)

References 57 publications

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“…It is not yet clear if fish possesses a common mucosal system or not. Till now specific homing of mucosal leucocytes has not been clearly detected [2,3], although suggestions on a homing model have been made by Fillatreau et al [22]. However, evidences indicate induction of specific antibodies in the skin mucus, but not in the serum, following oral vaccination [7,8].…”

Section: Mucosal Vs Systemic Antigen Responsesmentioning

confidence: 99%

“…The development of efficient mucosal adjuvants that can be applied e singly or in combination e via encapsulation is necessary to reduce the amount of required antigens for oral or immersion vaccination. In this context, biofilm vaccines or genetically modified plants, algae or fungi (cf [1,3]), allowing the combination of a vaccine component (i.e. a peptide) with adjuvant or immune-stimulatory molecule, should be considered.…”

Section: Mucosal Vaccinationsmentioning

confidence: 99%

“…Nevertheless, upon systemic immunisation these specific mucosal antibodies were not or hardly detected. This differential generation of specific antibodies and the new information on specific antibody-producing cells at mucosal sites after intestinal [3,11] or immersion [14,15] vaccination inspired many scientists to study mucosal structures in different teleosts. The present review focuses on the mucosal adaptive immune system in fish.…”

Section: Introductionmentioning

confidence: 99%

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Adaptive immune responses at mucosal surfaces of teleost fish

Rombout

Gao

Kiron

2014

Fish & Shellfish Immunology

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139

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This review describes the extant knowledge on the teleostean mucosal adaptive immune mechanisms, which is relevant for the development of oral or mucosal vaccines. In the last decade, a number of studies have shed light on the presence of new key components of mucosal immunity: a distinct immunoglobulin class (IgT or IgZ) and the polymeric Ig receptor (pIgR). In addition, intestinal T cells and their putative functions, antigen uptake mechanisms at mucosal surfaces and new mucosal vaccination strategies have been reported. New information on pIgR of Atlantic cod and common carp and comparison of natural and specific cell-mediated cytotoxicity in the gut of common carp and European seabass, is also included in this review. Based on the known facts about intestinal immunology and mucosal vaccination, suggestions are made for the advancement of fish vaccines.

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Section: Mucosal Vs Systemic Antigen Responsesmentioning

confidence: 99%

Section: Mucosal Vaccinationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Adaptive immune responses at mucosal surfaces of teleost fish

Rombout

Gao

Kiron

2014

Fish & Shellfish Immunology

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250

139

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“…The probiotic effects of this feed vaccine are clearly a unique advantage of this method in addition to the advantages of oral vaccines that overcome the drawbacks of injection vaccines [1e3, 39,40]. It is quite certain that the probiotic effects of the feed vaccine were due to L. lactis BFE920, which was used as a vaccine delivery vehicle.…”

Section: Discussionmentioning

confidence: 99%

Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing SiMA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus)

Kim

Beck

Lee

et al. 2016

Fish & Shellfish Immunology

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“…An oral vaccine for use in Atlantic salmon against IPNV is commercially available (Merck, AquaVac® IPN Oral). However, very few 'mucosal' vaccines are commercially available, most likely attributed to their variable efficacies (summarized by Rombout and Kiron, 2014). To the best of our knowledge, no oral (DNA) vaccine protective against SAV, however, has been tested in Atlantic salmon.…”

Section: Vaccination Methodsmentioning

confidence: 99%

Next-generation salmonid alphavirus vaccine development

Hikke¹

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Aquaculture is essential to meet the current and future demands for seafood to feed the world population. Atlantic salmon and rainbow trout are two of the most cultured aquaculture species. A pathogen that threatens these species is salmonid alphavirus (SAV). A current inactivated virus vaccine against SAV provides cross-protection against all SAV subtypes in salmonids and reduces mortality amongst infected fish. However, protection is not 100% and due to virus growth at low temperature, the vaccine production process is time consuming. In addition, the vaccine needs to be injected into the fish, which is a cumbersome process. The work described in this thesis aimed to increase the general knowledge of SAV and to assess current vaccine technologies, and to use this knowledge in designing next-generation vaccines for salmonid aquaculture.An alternative cell line to support SAV proliferation was identified, however, the virus production time could not yet outcompete the current SAV production system. Making use of the baculovirus insect cell expression system, multiple enveloped virus-like particle (eVLP), and core-like particle (CLP) prototype vaccines were produced in insect cells at high temperature. An in vivo vaccination study showed, however, that these vaccines could not readily protect Atlantic salmon against SAV. The low temperaturedependent replication of SAV was attributed to the glycoprotein E2, and it was found that E2 only correctly travelled to the cell surface at low temperature, and in the presence of glycoprotein E1. The biological impact of this finding was confirmed in the development and in vivo testing of a DNA-launched replicon vaccine. The effective DNAlaunched replicon vaccine was extended by delivery of the capsid protein in trans. It was hypothesized that viral replicon particles (VRP) were formed in vivo, which would cause an additional single round of infection and might further elevate the immune response in comparison to the replicon vaccine. A second animal trial indicated that the inclusion of capsid did not yet improve vaccine efficacy. This trial however did show that a DNA vaccine transiently expressing the SAV structural proteins provided superior protection over both replicon vaccines (with and without capsid).In this thesis, some virus characteristics, such as the cause of temperature-dependency of SAV replication, of an unique aquatic virus were further explored. The production and in vivo testing of multiple next-generation vaccines defined the prerequisites for induction of a potent immune response in Atlantic salmon. A prototype DNA-launched replicon vaccine has shown potential for further development. The research described in this thesis contributes to the development of next-generation vaccines in the challenging area of fish vaccinology. (European Commission, 2015). Aquaculture is indispensable, as wild-catch of seafood cannot meet the growing human consumption demands. In addition, aquaculture may allow the recovery of those wild fish stocks that have been ...

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Mucosal Vaccination of Fish

Cited by 25 publications

References 57 publications

Adaptive immune responses at mucosal surfaces of teleost fish

Adaptive immune responses at mucosal surfaces of teleost fish

Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing SiMA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus)

Next-generation salmonid alphavirus vaccine development

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