Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals

Arredondo‐Hernández, René; Schmulson, Max; Orduña, Patricia; López-Leal, Gamaliel; Zárate, Ángel-Mario; Alanis-Funes, Gerardo J.; Alcaraz, Luis David; Santiago-Cruz, Rubí; Cevallos, Miguel A.; Villa, Antonio; Rosales, Samuel Ponce-de-León; López‐Vidal, Yolanda; Humano, Consorcio Mexicano para el Estudio del Microbioma

doi:10.3389/fcimb.2020.00072

Cited by 5 publications

(5 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Cluster 3, Enterobacteria and other species were enriched in inflamed regions, as reported previously (27,28). The enrichment of Microbacterium in inflamed regions was detected as has been previously reported for IBD patients (29). Together, the abundance compositions of the top 50 taxa at the species level were similar between the inflamed regions of irAE and UC and the non-inflamed regions of irAE and UC, respectively.…”

Section: Hierarchical Clustering and Relative Abundance Of Taxasupporting

confidence: 85%

Intestinal Microbiota and Gene Expression Reveal Similarity and Dissimilarity Between Immune-Mediated Colitis and Ulcerative Colitis

et al. 2021

View full text Add to dashboard Cite

Immune checkpoint inhibitors (ICIs) have become the standard of care for several cancers. However, ICI therapy has also been associated with various immune-related adverse events (irAEs). Clinical manifestations of immune-related colitis resemble those of inflammatory bowel diseases such as ulcerative colitis (UC). The composition of the bowel microflora is thought to influence the development of inflammatory bowel disease and irAE colitis. We profiled the gene expressions and microbe compositions of colonic mucosa from patients with solid cancers receiving anti-PD-L1 antibody treatment; we then compared the expression profiles associated with irAE colitis with those associated with UC. The pathway enrichment analysis revealed functional similarities between inflamed regions of irAE colitis and UC. The common enriched pathways included leukocyte extravasation and immune responses, whereas non-inflamed mucosa from patients with irAE colitis was distinct from patients with UC and was characterized by the recruitment of immune cells. A similarity between the microbiota profiles was also identified. A decreased abundance of Bacteroides species was observed in inflamed regions from both irAE colitis and UC based on a microbiota composition analysis of 16S rDNA sequencing. Pathways associated with molecule transport systems, including fatty acids, were enriched in inflamed and non-inflamed irAE colitis and inflamed UC, similar to Piphillin-inferred KEGG pathways. While UC is characterized by local regions of inflammation, ICI treatment extends to non-inflammatory regions of the colonial mucosa where immune cells are reconstituted. This analysis of the similarity and heterogeneity of irAE colitis and UC provides important information for the management of irAE colitis.

show abstract

Section: Hierarchical Clustering and Relative Abundance Of Taxasupporting

confidence: 85%

Intestinal Microbiota and Gene Expression Reveal Similarity and Dissimilarity Between Immune-Mediated Colitis and Ulcerative Colitis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Among the most abundant species identified were Alteromonas mediterranea , a seawater bacteria 26 and Falconid herpesvirus 1 , a herpesvirus reported to infect raptors, 27 and their presence in seminal plasma seems somewhat unlikely. Alteromonas mediterranea has previously been found in colon samples from patients with irritable bowel syndrome, 28 but to our knowledge Falconid herpesvirus 1 has not been reported in human samples before and could reflect external contamination. On the other hand, contamination with both species also seems unlikely since their normal habitation is far from a laboratory setting.…”

Section: Discussionmentioning

confidence: 61%

The seminal plasma microbiome of men with testicular germ cell tumours described by small RNA sequencing

et al. 2022

View full text Add to dashboard Cite

Background: It has been estimated that microorganisms are involved in the pathogenesis of approximately 20% of all cancers. Testicular germ cell tumours (TGCTs) are the most common type of malignancy in young men and arise from the precursor cell, germ cell neoplasia in situ (GCNIS). The microbiome of seminal plasma and testicular tissue has not been thoroughly investigated in regard to TGCTs. Objectives:To investigate the differences in the seminal plasma microbiome between men with TGCT or GCNIS-only compared with controls. Materials and methods:The study population consisted of patients with GCNIS-only (n = 5), TGCT (n = 18), and controls (n = 25) with different levels of sperm counts in the ejaculate. RNA was isolated from the seminal plasma and sequenced. Reads not mapping to the human genome were aligned against a set of 2784 bacterial/archaeal and 4336 viral genomes using the Kraken pipeline. Results:We identified reads from 2172 species and most counts were from Alteromonas mediterranea, Falconid herpesvirus 1, and Stigmatella aurantiaca. Six species (Acaryochloris marina, Halovirus HGTV-1, Thermaerobacter marianensis, Thioalkalivibrio sp. K90mix, Burkholderia sp. YI23, and Desulfurivibrio alkaliphilus) were found in significantly (q-value < 0.05) higher levels in the seminal plasma of TGCT and GCNIS-only patients compared with controls. In contrast, Streptomyces phage VWB was found at significantly higher levels among controls compared with TGCT and GCNIS-only patients combined.Discussion: Often the microbiome is analysed by shotgun or 16S ribosomal sequencing whereas our present data build on small RNA sequencing. This allowed us to identify more viruses and phages compared with previous studies but also makes the results difficult to directly compare. Conclusion:Our study is the first to report identification of the microbiome species in seminal plasma of men with TGCT and GCNIS-only, which potentially could be involved in the pathogenesis of TGCTs. Further studies are, however, needed to confirm our findings.

show abstract

“… 40 , 54 , 55 Barrier function gene PARD3 has IBS SNPs. 45 , 56 PARD3 is positioned diurnally to the H3K9me2 repressive nuclear lamina-associated domains in human colon epithelial HCT116 cells. 41 , 45 We detected Pard3 promoter methylation and repressed transcription in our parallel WAS rat study.…”

Section: Discussionmentioning

confidence: 99%

Glucocorticoid receptor-mediated Nr1d1 chromatin circadian misalignment in stress-induced irritable bowel syndrome

Zheng¹,

Pang²,

Wang³

et al. 2023

iScience

View full text Add to dashboard Cite

Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals

Cited by 5 publications

References 36 publications

Intestinal Microbiota and Gene Expression Reveal Similarity and Dissimilarity Between Immune-Mediated Colitis and Ulcerative Colitis

Intestinal Microbiota and Gene Expression Reveal Similarity and Dissimilarity Between Immune-Mediated Colitis and Ulcerative Colitis

The seminal plasma microbiome of men with testicular germ cell tumours described by small RNA sequencing

Glucocorticoid receptor-mediated Nr1d1 chromatin circadian misalignment in stress-induced irritable bowel syndrome

Contact Info

Product

Resources

About