Mucosal expression of aquaporin‐4 in the stomach of histamine type 2 receptor knockout mice and <i><scp>H</scp>elicobacter pylori</i>‐infected mice

Fukuhara, Seiichiro; Matsuzaki, Juntaro; Tsugawa, Hitoshi; Masaoka, Tatsuhiro; Miyoshi, Sawako; Mori, Hideki; Fukushima, Yasushi; Yasui, Masato; Kanai, Takanori; Suzuki, Hidekazu

doi:10.1111/jgh.12771

Cited by 14 publications

(13 citation statements)

References 32 publications

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“…AQP4 and KCNQ1-positive parietal cells, and dilated mucous glands were found broadly from the basal side to the apical side of the mucosa. The extension of the distribution of AQP4 and KCNQ1-positive cells toward the surface of the fundic glands was observed according to the previous report [ 10 , 11 ]. These findings differed from the usual FGPs, and were thought to be lesions associated with long-term PPI administration.…”

Section: Discussionsupporting

confidence: 69%

Hemorrhagic polyps formed like fundic gland polyps during long-term proton pump inhibitor administration

Takeda

Asaoka

Tajima

et al. 2017

Clin J Gastroenterol

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We report a rare case of hemorrhagic gastric polyps resulting in anemia during long-term proton pump inhibitor (PPI) administration that endoscopically looked like a fundic gland polyp (FGP). A 44-year-old man presented complaining of anemia and tarry stools. Esophagogastroduodenoscopy (EGD) demonstrated multiple white edematous polyps in the corpus and antrum, which were considered to be FGPs. We attempted endoscopic hemostasis but hemorrhaging increased because of hemorrhagic polyps and vulnerable gastric mucosa. Re-bleeding occurred several times. Polyp resection was performed at 24 polyp sites. We also ceased the administration of PPI. Microscopically, polyps showed characteristics of hyperplasia in the foveolar epithelium, extensions of fundic glands, and edema of the stroma. The proliferation of parietal and chief cells was also observed. Immunohistochemically, aquaporin-4 (AQP4) and KCNQ1-positive parietal cells and dilated mucous glands were found from the basal side to the apical side of the mucosa. These findings were compatible with the development of lesions associated with the long-term administration of PPI. EGD revealed an improvement in the vulnerability of gastric mucosa and the development of polyps, with no further gastric polyps observed 1 year after discharge. Bleeding from polyps resembling FGPs is generally rare, with indications that long-term PPI administration may induce such bleeding.

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Section: Discussionsupporting

confidence: 69%

Hemorrhagic polyps formed like fundic gland polyps during long-term proton pump inhibitor administration

Takeda

Asaoka

Tajima

et al. 2017

Clin J Gastroenterol

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“…As shown in Table 1 , at least six isoforms of AQPs (AQP1, AQP3, AQP4, AQP5, AQP6 and AQP8) are present in the stomach mucosa of murine rodents, as demonstrated by the methods of reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, ELISA, in situ hybridization, immunofluorescence and immunohistochemistry [ 18 , 19 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. These AQPs are mainly present in the gastric mucosa and glands, and they have been mostly reported in rats ( Table 2 ).…”

Section: Expression Distribution and Role Of Aqps In The Stomach mentioning

confidence: 99%

“…By using immunohistochemistry, AQP3 protein was detected in the basolateral membrane of glandular stomach [ 32 ]. Moreover, AQP4 expression has been demonstrated in the stomach of rats, mice and guinea pigs, but with different distributed localizations [ 31 , 33 , 34 , 35 , 36 , 37 ]. In Sprague-Dawley rats, AQP4 protein is expressed not only in gastric mucosa [ 31 ], but also in the basolateral membranes of mature parietal cells in specific locations along the axis of the gastric glands [ 34 ].…”

Section: Expression Distribution and Role Of Aqps In The Stomach mentioning

confidence: 99%

“…In Sprague-Dawley rats, AQP4 protein is expressed not only in gastric mucosa [ 31 ], but also in the basolateral membranes of mature parietal cells in specific locations along the axis of the gastric glands [ 34 ]. In mice, AQP4-positive parietal cells are distributed in the basal side of gastric fundic mucosa [ 35 ], especially in the basolateral membrane of parietal cells [ 36 , 37 ]. AQP4 protein is expressed in parietal cells of the stomach in guinea pig [ 33 ].…”

Section: Expression Distribution and Role Of Aqps In The Stomach mentioning

confidence: 99%

“…A recent study has suggested that AQP3 is involved in Helicobacter py lori infection-related gastric carcinogenesis since AQP3 expression was upregulated both in human gastric adenocarcinoma cells and in rat gastric tissues [ 49 ]. However, in the Helicobacter py lori -infected mice, the ratio between AQP4 and H + /K + ATPase mRNA expression was significantly decreased in histamine type 2 receptor knockout mice [ 35 ]. Notably, the protective effect of calcitonin gene-related peptide on gastric mucosa injury in rats was demonstrated to be involved in inhibition of AQP4 expression and mast cell degranulation and in regulation of several hormone genes’ expression (such as gastrin and somatostatin) [ 50 ].…”

Section: Expression Distribution and Role Of Aqps In The Stomach mentioning

confidence: 99%

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Expression, Distribution and Role of Aquaporin Water Channels in Human and Animal Stomach and Intestines

Zhu

Chen

Jiang

2016

IJMS

123

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Stomach and intestines are involved in the secretion of gastrointestinal fluids and the absorption of nutrients and fluids, which ensure normal gut functions. Aquaporin water channels (AQPs) represent a major transcellular route for water transport in the gastrointestinal tract. Until now, at least 11 AQPs (AQP1–11) have been found to be present in the stomach, small and large intestines. These AQPs are distributed in different cell types in the stomach and intestines, including gastric epithelial cells, gastric glands cells, absorptive epithelial cells (enterocytes), goblet cells and Paneth cells. AQP1 is abundantly distributed in the endothelial cells of the gastrointestinal tract. AQP3 and AQP4 are mainly distributed in the basolateral membrane of epithelial cells in the stomach and intestines. AQP7, AQP8, AQP10 and AQP11 are distributed in the apical of enterocytes in the small and large intestines. Although AQP-null mice displayed almost no phenotypes in gastrointestinal tracts, the alterations of the expression and localization of these AQPs have been shown to be associated with the pathology of gastrointestinal disorders, which suggests that AQPs play important roles serving as potential therapeutic targets. Therefore, this review provides an overview of the expression, localization and distribution of AQPs in the stomach, small and large intestine of human and animals. Furthermore, this review emphasizes the potential roles of AQPs in the physiology and pathophysiology of stomach and intestines.

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Altered glycosylation in secreting cells of the gastric glands of aquaporin‐4‐deficient mice

Mentino,

Nicchia,

Frigeri

et al. 2024

Microscopy Res & Technique

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Aquaporins (AQPs) are important for water transport in the gastrointestinal tract. Changes in their expression and/or localization could cause in disorders and be used as therapeutic targets. Aquaporin‐4 (AQP4) is expressed predominantly on the basolateral membrane of the parietal cells in the corpus of the murine gastric glands. Although the secretion of gastric juice is not affected in AQP4‐deficient knockout, we evaluated by light microscopy whether the lack of AQP4 affects the glycopatterns of secreting gastric cells. Wild type (WT) and AQP4‐deficient knockout mice (KO) were fed a standard diet ad libitum before sacrifice. Segments of stomach corpus were collected, fixed in buffered formalin, and embedded in paraffin wax. Sections, 5‐μm thick, were analyzed by histochemical methods (Periodic acid‐Schiff, Alcian Blue pH 2.5), and binding of lectins specific to GalNAc (SBA, DBA), Gal (PNA) GlcNAc (WGA, GSAII) mannose and/or glucose (ConA), and fucose (UEA‐I, AAA, LTA). Immunohistochemical methods such as anti‐Muc6 for neck cells and anti‐ β‐ H+/K+‐ATPase for parietal cells were also performed. Compared to WT mice, in the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated, and fucosylated residues were observed; lower fucosylation resulted also in the parietal cells. The observed differences of KO in respect to WT could lead to severer pathological conditions.Research Highlights Glycopatterns in gastric glands were compared between wild type (WT) and AQP4‐deficient knockout (KO) mice by histochemical and lectin‐binding methods. In the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated and fucosylated residues were observed. In the parietal cells lower fucosylation also resulted. AQP4‐deficiency affects glycosylation and could result in altered functionality and pathological conditions.

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Mucosal expression of aquaporin‐4 in the stomach of histamine type 2 receptor knockout mice and Helicobacter pylori‐infected mice

Cited by 14 publications

References 32 publications

Hemorrhagic polyps formed like fundic gland polyps during long-term proton pump inhibitor administration

Hemorrhagic polyps formed like fundic gland polyps during long-term proton pump inhibitor administration

Expression, Distribution and Role of Aquaporin Water Channels in Human and Animal Stomach and Intestines

Altered glycosylation in secreting cells of the gastric glands of aquaporin‐4‐deficient mice

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