Mucosal DNA vaccination with highly attenuated Shigella is superior to attenuated Salmonella and comparable to intramuscular DNA vaccination for T cells against HIV

Vecino, William; Morin, Paul; Agha, Rabia; Jacobs, William R.; Fennelly, Glenn J.

doi:10.1016/s0165-2478(02)00043-3

Cited by 33 publications

(23 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The memory responses of these mice could be recalled 8 months after immunization (our unpublished work). Similar results were described in studies after immunization with S. flexneri 15D (asd 2 ) (Vecino et al, 2002) or CVD1203 (aroA 2 iscA 2 ) , or S. typhimurium SL7207 (aro 2 his 2 ) host carrying HIV env DNA vaccines , showing that the cellular immune responses generated with bacterial carriers are comparable to those after intramuscular naked DNA immunization. While immunization studies with Shigella carriers were usually done intranasally, an intrarectal immunization was also be effective in eliciting mucosal and systemic responses (see Table I).…”

Section: Immune Responsessupporting

confidence: 82%

“…These studies showed that immune responses could be generated against antigens from various sources, including bacterial antigens from L. monocytogenes (Darji et al, 2000), Chlamydia trachomatis (Brunham and Zhang, 1999), Clostridium tetani (Pasetti et al, 1999;Anderson et al, 2000) and Mycobacterium tuberculosis (Mollenkopf et al, 2001); viral antigens from HSV (Flo et al, 2001;Elias and Flo, 2002), HBV (Woo et al, 2001), HCV (Wedemeyer et al, 2001), HIV Devico et al, 2002;Vecino et al, 2002;Xu et al, 2003), and measles virus (Fennelly et al, 1999), and tumor specific antigens from fibrosarcoma (Paglia et al, 1998), renal carcinoma , melanoma Niethammer et al, 2001b;Weth et al, 2001;Cochlovius et al, 2002), neuroblastoma Pertl et al, 2003), colon (Xiang et al, 2001a,b) and lung adenocarcinoma ; and a parasite antigen from Penicillium marneffi (Wong et al, 2002). Immunizations were typically administrated orally with S. typhimurium and intranasally with Shigella, and the immune responses could be detected at both mucosal and systemic sites.…”

Section: Immune Responsesmentioning

confidence: 99%

See 1 more Smart Citation

AttenuatedSalmonellaandShigellaas Carriers for DNA Vaccines

Ulmer

2003

Journal of Drug Targeting

View full text Add to dashboard Cite

The discovery that genes can be functionally transferred from bacteria to mammalian cells has suggested the possible use of bacterial vectors as gene delivery vehicles for vaccines. Attenuated invasive human intestinal bacteria, such as Salmonella and Shigella, have been used as plasmid DNA vaccine carriers and their potency has been evaluated in several animal models. This delivery system allows the administration of DNA vaccines together with associated bacterial immunostimulators directly to professional antigen presenting cells via human mucosal surfaces. Various strategies have been taken to improve the use of this delivery system to achieve robust immune responses at both mucosal and systemic sites of the immunized animals.

show abstract

Section: Immune Responsessupporting

confidence: 82%

Section: Immune Responsesmentioning

confidence: 99%

AttenuatedSalmonellaandShigellaas Carriers for DNA Vaccines

Ulmer

2003

Journal of Drug Targeting

View full text Add to dashboard Cite

show abstract

“…3). Viable S. typhimurium SL7207 can be recovered from the lungs and spleens of mice several days after in vivo inoculation [Vecino et al, 2002].…”

Section: Discussionmentioning

confidence: 99%

Eukaryotic Promoters Can Direct Protein Synthesis in Gram-Negative Bacteria

2003

View full text Add to dashboard Cite

Intracellular bacteria can act as DNA delivery vectors into mammalian cells. Transfer of genetic information can be monitored by screening for cellular expression of a reporter gene under the control of an eukaryotic promoter. However, intracellular bacteria can also efficiently deliver heterologous proteins in the cell cytosol. We have studied the activity of the eukaryotic PCMV promoter in Escherichia coli and Salmonellatyphimurium using the lacZ and gfp genes as reporters and determined its strength relative to those of PRSV and PSV40 in E. coli. We found substantial heterologous activity of fragments carrying the PCMV and PRSV promoters, the strength of PRSV being greater than that of PCMV, but did not detect any PSV40 activity in E. coli. The green fluorescent protein (GFP) synthesized in E. coli was transferred to COS-1 cells where it was detectable and stable. Insertion of a transcription terminator or deletion of the bacterial ribosome binding site downstream from PCMV led to the silencing of the promoter in bacteria but not in mammalian cells. These observations should incite to exert caution when interpreting data on the DNA transfer from bacteria to mammallian cells when the genes of interest are under the control of the PCMV or PRSV promoter.

show abstract

“…DNA immunization is attractive for its advantages over traditional vaccines, and is discussed in elsewhere [21][22][23][24] . We have reported that HBV DNA vaccine NV-HB/s could express HBsAg in muscle cells after injecting into mouse muscle, followed by the induction of an immune response, including the switchover of anti-HBs production in peripheral blood [12,13] .…”

Section: Discussionmentioning

confidence: 99%

Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells

Qin

Tang²,

Zhao³

et al. 2003

WJG

View full text Add to dashboard Cite

AIM:To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/ Flag, and to express HBsAg S, MS, and LS proteins in SP2/ 0 cells, and to establish monoclone SP2/0 cell strains that are capable of expressing S or S2S proteins stably. METHODS:Segments of S, preS2-S, preS1-preS2-S genes of Hepatitis B virus were amplified by routine PCR and preS1-S fragment was amplified by Over-Lap Extension PCR. The amplified segments were cleaved with restricted endonuclease Hind III/Not I followed by ligation with pRc/ CMV, or BamHI/EcoR I followed by ligation with pSG5UTPL/ Flag. After the plasmid vectors were cleaved with the correspond enzymes, the amplified segments were inserted into pRc/CMV or pSG5UTPL/Flag plasmid vectors with T4 DNA ligase. KOZAK sequence was added before the initial ATG code of each fragment using specific primer. The inserted segments in the recombinant plasmids were sequenced after subcloning. BALB/c mice myeloma cells (SP2/0 cell line) were transfected with the recombinant plasmids. The expressions of the different recombinants were Compared by Western-blot, using a monoclonal anti-HBs antibody as the primary antibody and peroxidase-labeled multi-linker as the secondary. Stable SP2/0-pRc/CMV-S or SP2/0-pRc/CMV-MS clones were established through clone screening with G418.

show abstract

Mucosal DNA vaccination with highly attenuated Shigella is superior to attenuated Salmonella and comparable to intramuscular DNA vaccination for T cells against HIV

Cited by 33 publications

References 25 publications

AttenuatedSalmonellaandShigellaas Carriers for DNA Vaccines

AttenuatedSalmonellaandShigellaas Carriers for DNA Vaccines

Eukaryotic Promoters Can Direct Protein Synthesis in Gram-Negative Bacteria

Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells

Contact Info

Product

Resources

About