Mucosal Disease Caused by Leishmania Braziliensis Guyanensis

Santrich, Cecilia; Segura, Iris; Arias, Alba Lucia; Saravia, Nancy Gore

doi:10.4269/ajtmh.1990.42.51

Cited by 55 publications

(30 citation statements)

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“…While a previous study performed at FMT-HVD in the 1990s demonstrated the predominance of L. (V.) guyanensis among cases of cutaneous leishmaniasis (22) , the relative frequency of the mucosal impairment caused by this species remains unknown. Furthermore, consistent with recent fi ndings in Brazil (5) , cases of ML caused by L. (V.) guyanensis and L.(V.) panamensis were recently described in Colombia (23) (24) . In the present study, PCR-RFLP analysis detected a higher number (17) .…”

Section: Ethical Considerationssupporting

confidence: 86%

Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin

Prestes

Guerra

Romero

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction:In the Americas, mucosal leishmaniasis is primarily associated with infection by Leishmania (Viannia) braziliensis. However, Leishmania (Viannia) guyanensis is another important cause of this disease in the Brazilian Amazon. In this study, we aimed at detecting Leishmania deoxyribonucleic acid (DNA) within paraffi n-embedded fragments of mucosal tissues, and characterizing the infecting parasite species. Methods: We evaluated samples collected from 114 patients treated at a reference center in the Brazilian Amazon by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses. Results: Direct examination of biopsy imprints detected parasites in 10 of the 114 samples, while evaluation of hematoxylin and eosin-stained slides detected amastigotes in an additional 17 samples. Meanwhile, 31/114 samples (27.2%) were positive for Leishmania spp. kinetoplast deoxyribonucleic acid (kDNA) by PCR analysis. Of these, 17 (54.8%) yielded amplifi cation of the mini-exon PCR target, thereby allowing for PCR-RFLP-based identifi cation. Six of the samples were identifi ed as L. (V.) braziliensis, while the remaining 11 were identifi ed as L. (V.) guyanensis. Conclusions: The results of this study demonstrate the feasibility of applying molecular techniques for the diagnosis of human parasites within paraffi nembedded tissues. Moreover, our fi ndings confi rm that L. (V.) guyanensis is a relevant causative agent of mucosal leishmaniasis in the Brazilian Amazon.

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Section: Ethical Considerationssupporting

confidence: 86%

Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin

Prestes

Guerra

Romero

et al. 2015

Rev. Soc. Bras. Med. Trop.

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“…35 Leishmania (Viannia) braziliensis is the most well-represented causative species in ML. 1,35,36 We have demonstrated that related species including L. (V.) guyanensis, L. (V.) panamensis, and L. (V.) peruviana are implicated in noncontiguous metastatic mucosal disease as well, though in this study, the ML that they caused was confined to the nasal mucosa. Interestingly, all primary ML in this study was caused by either L. (V.) braziliensis or hybrid/mixed species.…”

Section: Discussionmentioning

confidence: 62%

Quantitative Kinetoplast DNA Assessment During Treatment of Mucosal Leishmaniasis as a Potential Biomarker of Outcome: A Pilot Study

Jara¹,

Valencia²,

Adaui³

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. Mucosal leishmaniasis (ML) is a disfiguring manifestation of Leishmania (Viannia) infection. We evaluated parasite load (PL) over time as a potential biomarker of treatment outcome in ML. PL was assessed with kinetoplast DNA quantitative real-time polymerase chain reaction (kDNA-qPCR) at enrollment, days 14 and 21-28 of therapy and 3, 6, 12-18, and 18-24 months after treatment of ML and correlated to demographic, clinical, and parasitologic factors. Forty-four patients were enrolled: 30 men and 14 women. Enrollment PL differed significantly by causative species (P < 0.001), and was higher in patients with severe ML (nasal and laryngeal involvement) compared with those with only isolated nasal involvement (median = 1,285 versus 51.5 parasites/μg tissue DNA; P = 0.005). Two patterns of PL emerged: pattern 1 (N = 23) was characterized by a sequential decline in PL during and after therapy until kDNA was undetectable. Pattern 2 (N = 18) was characterized by clearance of detectable kDNA during treatment, followed by an increased PL thereafter. All patients who failed treatment (N = 4) demonstrated pattern 1. Leishmania (Viannia) braziliensis was overrepresented among those with pattern 2 (P = 0.019). PL can be quantified by cytology brush qPCR during and after treatment in ML. We demonstrate that treatment failure was associated with undetectable PL, and L. (V.) braziliensis infection was overrepresented in those with rebounding PL.

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“…3,4 New World CL generally necessitates treatment because of the possibility of future disfiguring mucosal involvement if left untreated. 5,6 However, definitive diagnosis of CL remains challenging, particularly in resourcelimited areas where the disease is highly endemic. Although molecular tests are sensitive and becoming increasingly popular (especially in research settings), [7][8][9][10][11] definitive diagnosis still rests on demonstration of the parasite by microscopy or culture, both of which are widely used in clinical laboratories.…”

Section: Introductionmentioning

confidence: 99%

Clinical and Demographic Stratification of Test Performance: A Pooled Analysis of Five Laboratory Diagnostic Methods for American Cutaneous Leishmaniasis

Boggild¹,

Ramos²,

Espinosa³

et al. 2010

The American Journal of Tropical Medicine and Hygiene

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Mucosal Disease Caused by Leishmania Braziliensis Guyanensis

Cited by 55 publications

References 0 publications

Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin

Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin

Quantitative Kinetoplast DNA Assessment During Treatment of Mucosal Leishmaniasis as a Potential Biomarker of Outcome: A Pilot Study

Clinical and Demographic Stratification of Test Performance: A Pooled Analysis of Five Laboratory Diagnostic Methods for American Cutaneous Leishmaniasis

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