Mucosal co-administration of cholera toxin and influenza virus hemagglutinin-DNA in ponies generates a local IgA response

Soboll, Gisela; Nelson, Kathryn M.; Leuthner, E.S.; Clark, Rodney; Drape, Robert J.; Macklin, Michael D.; Swain, William F.; Olsen, Christopher W.; Lunn, D.P.

doi:10.1016/s0264-410x(03)00161-0

Cited by 27 publications

(17 citation statements)

References 25 publications

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“…Limitations that may arise after mucosal DNA vaccination may be overcome, potentiating its effectiveness by adsorption of DNA into positively charged polylactide-coglycolide microparticles (19), or codelivering the DNA that encodes for the specific Ag with DNA vectors encoding cytokines (20). Also, coadministration of DNA vectors with mucosal adjuvants have been proved to be as an efficient strategy, giving protection against equine influenza in a pony model (21).…”

Section: Induction Of Hiv Immunity In the Genitalmentioning

confidence: 99%

Induction of HIV Immunity in the Genital Tract After Intranasal Delivery of a MVA Vector: Enhanced Immunogenicity After DNA Prime-Modified Vaccinia Virus Ankara Boost Immunization Schedule

Gherardi

Pérez-Jiménez

Nájera

et al. 2004

The Journal of Immunology

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Vaccines intended to prevent mucosal transmission of HIV should be able to induce multiple immune effectors in the host including Abs and cell-mediated immune responses at mucosal sites. The aim of this study was to characterize and to enhance the immunogenicity of a recombinant modified vaccinia virus Ankara (MVA) expressing HIV-1 Env IIIB Ag (MVAenv) inoculated in BALB/c mice by mucosal routes. Intravaginal inoculation of MVAenv was not immunogenic, whereas intranasally it induced a significant immune response to the HIV Ag. Intranasal codelivery of MVAenv plus cholera toxin (CT) significantly enhanced the cellular and humoral immune response against Env in the spleen and genitorectal draining lymph nodes, respectively. Heterologous DNAenv prime-MVAenv boost by intranasal immunization, together with CT, produced a cellular immune response in the spleen 10-fold superior to that in the absence of CT. A key finding of these studies was that both MVAenv/MVAenv and DNAenv/MVAenv schemes, plus CT, induced a specific mucosal CD8+ T cell response in genital tissue and draining lymph nodes. In addition, both immunizations also generated systemic Abs, and more importantly, mucosal IgA and IgG Abs in vaginal washings. Specific secretion of β-chemokines was also generated by both immunizations, with a stronger response in mice immunized by the DNA-CT/MVA-CT regimen. Our findings are of relevance in the area of vaccine development and support the optimization of protocols of immunization based on MVA as vaccine vectors to induce mucosal immune responses against HIV.

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Section: Induction Of Hiv Immunity In the Genitalmentioning

confidence: 99%

Induction of HIV Immunity in the Genital Tract After Intranasal Delivery of a MVA Vector: Enhanced Immunogenicity After DNA Prime-Modified Vaccinia Virus Ankara Boost Immunization Schedule

Gherardi

Pérez-Jiménez

Nájera

et al. 2004

The Journal of Immunology

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“…Second, protective immunity to equine influenza is likely to involve a mucosal immunoglobulin A (IgA) response which is not seen with traditional intramuscularly administered vaccines (19). Equine influenza virus replicates in the nasal mucosa, and thus an intranasally administered vaccine may be a preferable route of inoculation to elicit this response (31). Finally, live vaccines also have the advantage of intranasal administration, which avoids the swelling and muscle soreness occasionally associated with the intramuscular administration of inactivated adjuvanted vaccines.…”

mentioning

confidence: 99%

Attenuation of Equine Influenza Viruses through Truncations of the NS1 Protein

Quinlivan

Zamarin

Garcı́a-Sastre

et al. 2005

J Virol

214

224

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Equine influenza is a common disease of the horse, causing significant morbidity worldwide. Here we describe the establishment of a plasmid-based reverse genetics system for equine influenza virus. Utilizing this system, we generated three mutant viruses encoding carboxy-terminally truncated NS1 proteins. We have previously shown that a recombinant human influenza virus lacking the NS1 gene (delNS1) could only replicate in interferon (IFN)-incompetent systems, suggesting that the NS1 protein is responsible for IFN antagonist activity. Contrary to previous findings with human influenza virus, we found that in the case of equine influenza virus, the length of the NS1 protein did not correlate with the level of attenuation of that virus. With equine influenza virus, the mutant virus with the shortest NS1 protein turned out to be the least attenuated. We speculate that the basis for attenuation of the equine NS1 mutant viruses generated is related to their level of NS1 protein expression. Our findings show that the recombinant mutant viruses are impaired in their ability to inhibit IFN production in vitro and they do not replicate as efficiently as the parental recombinant strain in embryonated hen eggs, in MDCK cells, or in vivo in a mouse model. Therefore, these attenuated mutant NS1 viruses may have potential as candidates for a live equine influenza vaccine.

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“…Research has focused on exploring alternative vaccine technologies that can confer broad, long-lived protection against infection (44), with more efficient production methods (64). Recent studies have utilized modified live viruses (MLV)(8,9, 35, 57), pox virus vectors (5, 45, 54), DNA technology (35), and adjuvants (21, 55) to develop improved vaccines, some of which are now in commercial use.…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity and clinical protection against equine influenza by DNA vaccination of ponies

Ault¹,

Zajac²,

Kong³

et al. 2012

Vaccine

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Mucosal co-administration of cholera toxin and influenza virus hemagglutinin-DNA in ponies generates a local IgA response

Cited by 27 publications

References 25 publications

Induction of HIV Immunity in the Genital Tract After Intranasal Delivery of a MVA Vector: Enhanced Immunogenicity After DNA Prime-Modified Vaccinia Virus Ankara Boost Immunization Schedule

Induction of HIV Immunity in the Genital Tract After Intranasal Delivery of a MVA Vector: Enhanced Immunogenicity After DNA Prime-Modified Vaccinia Virus Ankara Boost Immunization Schedule

Attenuation of Equine Influenza Viruses through Truncations of the NS1 Protein

Immunogenicity and clinical protection against equine influenza by DNA vaccination of ponies

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