Mucosal bacterial microﬂora and mucus layer thickness inadolescents with inﬂammatory bowel disease

Fyderek, Krzysztof; Strus, Magdalena; Kowalska-Duplaga, Kinga; Gosiewski, Tomasz; Wędrychowicz, Andrzej; Jedynak-Wąsowicz, Urszula; Sładek, Małgorzata; Pieczarkowski, Stanisław; Adamski, Paweł; Kochan, Piotr; Heczko, Piotr B

doi:10.3748/wjg.15.5287

Cited by 125 publications

(83 citation statements)

References 37 publications

(35 reference statements)

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“…In addition, the mean numbers of culturable bifidobacteria were also lower in both UC and CD patients than in the control children, which is in agreement with previous studies. 14,41,44,46 Moreover, the diversity of bifidobacterial populations, as detected with bif-DGGE, was higher in the control children than in the UC and CD patients [p < 0.05 for all control vs disease groups]. Gevers et al 45 found that the imbalance in the microbial community was only observed in the microbiome profiles obtained from tissue samples, and was not seen in the fecal samples collected at the time of the diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Altered Fecal Microbiota in Paediatric Inflammatory Bowel Disease

Maukonen

Kolho

Paasela

et al. 2015

ECCOJC

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Background and Aims: Several factors support the view of inflammatory bowel disease [IBD] origin in the host responsiveness to intestinal bacteria, although no single bacterial species has been shown as a causative agent in the pathogenesis. Our aim was to analyse the fecal microbiota of paediatric IBD patients at different stages of the disease. In addition, the characteristics of immune response to the bacterial isolates showing very low abundance in IBD were studied. Methods: Fecal samples [1-3 samples/child] were collected from 10 paediatric patients with crohn's disease [CD],] and 12 with ulcerative colitis [UC] and from 8 healthy children, for polyphasic microbiological analysis (culture, real-time polymerase chain reaction [PCR], and denaturing gradient gel electrophoresis). In addition, in vitro cytokine responses of peripheral blood mononuclear cells to the bacterial isolates, which showed very low abundance in IBD, were studied. Results: Although predominant bacterial diversity was higher in IBD, the numbers of Lachnospiraceae and Coriobacteriaceae bacteria were lower in IBD patients as compared with control children [p < 0.05]. In addition, Ruminococcaceae population diversity was lower in IBD [p < 0.05] and correlated negatively with fecal calprotectin levels. Both abundance and diversity of bifidobacterial populations were lower in children with IBD [p < 0.05], and particularly low numbers of certain bifidobacterial isolates were detected. In CD, we found enhanced up-regulation of interleukin-6 transcripts and impaired RAR-related orphan receptor C response to bifidobacteria, whereas decreased interferon-gamma response was observed in both CD and UC. Conclusion: We demonstrate altered fecal microbiota in paediatric IBD, particularly low numbers and diversity of bifidobacterial populations. Interestingly, immunological response to bifidobacteria differed between paediatric CD patients and control children.

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Section: Discussionmentioning

confidence: 99%

Altered Fecal Microbiota in Paediatric Inflammatory Bowel Disease

Maukonen

Kolho

Paasela

et al. 2015

ECCOJC

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“…Some evidences have shown that in a pathological intestine, the thickness of the mucus layer is reduced, resulting in a closer contact between bacteria in the intestinal lumen and the intestinal epithelium, which may induce inflammatory processes in the bowel (Fyderek et al, 2009). In our study, no changes between the thickness of the mucus layer of the control and treatment groups were observed.…”

Section: Discussionmentioning

confidence: 99%

<i>Lactobacillus </i>Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler

et al. 2012

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Lactobacillus fermentum 1.2029 strain with high adhesion was selected for studying the mucin expression of the small intestine epithelium of broilers after the strain was orally administrated. The strain was allowed to adhere to Caco-2 and to a hydrophobic solvent to evaluate its adhesive properties. Adhesion to intestinal mucin of chickens was also tested to further characterize the in vivo adhesion of the strain. Afterward, the acid and bile salt tolerance of the strain was also evaluated. L fermentum 1.2029 showed good adhesive ability and was able to survive in low pH conditions and in the presence of bile salts. The in vivo study showed that the strain significantly increased goblet cell density in the jejunum and the level of MUC2 mRNA in both the jejunum and ileum (P＜0.05). L. fermentum 1.2029 treatment didn't affect the thickness of the mucous layer. These results demonstrate that L. fermentum 1.2029 has protective functions, thereby regulating the goblet cell density and the expression of mucin in the small intestine of broilers. This work also suggests that L. fermentum 1.2029 is a potential probiotic feed additive for the improvement of the intestinal function of chickens.

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“…Dysbiosis in the intestinal microbiota of persons with IBD has been described, but there are still varied reports on changes in the abundance of Bifidobacterium and Lactobacillus organisms in patients with IBD. We have shown that, among others, the numbers of Bifidobacteria are low in children with early, acute UC [15]. Wang [16] concluded recently that Bifidobacteria and Lactobacilli are increased in patients with active IBD after application of probiotics.…”

Section: Discussionmentioning

confidence: 68%

Influence of Lactobacillus and Bifidobacterium Combination on the Gut Microbiota, Clinical Course, and Local Gut Inflammation in Patients with Ulcerative Colitis: A Preliminary, Single-center, Open-label Study

Zurek¹,

Wcisło²,

Mach³

et al. 2017

J Prob Health

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Background: Ulcerative colitis (UC) is one of the chronic, relapsing, inflammatory disorders of the gut and is characterized by inflammation limited in most cases to the colon. Since gut microbiota play a critical role in the development and perpetuation of intestinal inflammation, the addition of probiotics to this complex system may exert a positive influence on gut inflammatory reactions. Methods:A single center, open-label, intention-to-treat study involving patients with moderate-to-severe UC was performed to check whether a probiotic mixture containing Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium longum given together with a standard treatment could decrease clinical and histopathology indexes for UC evaluation. Results:The mixture given once a day for at least 2 months together with mesalazine and ciprofloxacin to patients in the acute phase of UC significantly reduced their Mayo Clinic Index values. Moreover, numbers of Lactobacilli isolated from patients feces were significantly increased, while those of Gram-negative rods decreased. The mixture given together with mesalazine to patients with UC in remission also caused a decrease of their clinical scores, but a more prominent and significant decrease of the histopathological index values in biopsy samples was observed. Conclusions:Supplementation of standard therapy with the probiotic mixture used in this study was efficacious in inducing and maintaining remission in UC, and this effect was related to modulation of dysbiosis in the gut microbiota. analyzed randomized clinical trials and found that selected probiotics were efficacious in inducing and maintaining remission in UC [8]. Another review reached nearly the same conclusions on the efficacy of probiotics in UC [9]. Influence of Lactobacillus and Bifidobacterium Combination on the Gut• The aim of this study was to investigate in a single center, openlabel, intention-to-treat study involving patients with moderateto-severe UC whether a probiotic mixture containing Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium longum given together with standard therapy could decrease clinical and histopathology indexes for UC. Materials and Methods PatientsAltogether, 51 patients (21 with UC in remission and 30 in the active phase of UC) of both sexes and between 18 and 72 years of age who were being treated in the Jagiellonian University Medical College Clinic of Gastroenterology in Cracow, Poland were enrolled. The study was conducted from 2008 to 2011 and was approved by the Jagiellonian University independent ethics committee (No KBET/5/B/2007). Written informed consent was obtained from all patients before enrollment.Inclusion criteria included an existing diagnosis of UC based on disease history, colonoscopy results, and histopathologic evaluation of biopsies from the colonic mucosa. The patients were not treated with antibiotics for three months before enrollment. Exclusion criteria were: diabetes, autoimmune diseases, severe systemic diseases, alcoho...

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Mucosal bacterial microﬂora and mucus layer thickness inadolescents with inﬂammatory bowel disease

Cited by 125 publications

References 37 publications

Altered Fecal Microbiota in Paediatric Inflammatory Bowel Disease

Altered Fecal Microbiota in Paediatric Inflammatory Bowel Disease

<i>Lactobacillus </i>Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler

Influence of Lactobacillus and Bifidobacterium Combination on the Gut Microbiota, Clinical Course, and Local Gut Inflammation in Patients with Ulcerative Colitis: A Preliminary, Single-center, Open-label Study

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