Mucosal adjuvants and anti-infection and anti-immunopathology vaccines based on cholera toxin, cholera toxin B subunit and CpG DNA

Holmgren, Jan; Adamsson, Jenni; Anjuère, Fabienne; Clemens, John D.; Czerkinsky, Cécil; Eriksson, Kristina; Flach, Carl-Fredrik; George-Chandy, Annie; Harandi, Ali M.; Lebens, Michael; Lehner, Thomas; Lindblad, Marianne; Nygren, Erik; Raghavan, Sukanya; Sánchez, Jesús R.; Stanford, Michael; Sun, Jiabin; Svennerholm, Ann‐Mari; Tengvall, Sara

doi:10.1016/j.imlet.2004.11.009

Cited by 169 publications

(73 citation statements)

References 35 publications

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“…However, it is commonly reported that most heterologous proteins accumulate in plant biomass at much lower levels than 1% of the total soluble protein [17,21]. To improve the immunogenicity of the small amount of antigen protein expressed in transformed plants, plant-derived vaccines require efficient antigen delivery and adjuvant systems to present the appropriate antigens to the mucosal immune system [22].…”

Section: Introductionmentioning

confidence: 99%

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

2010

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Transgenic plants have been used as a safe and economic expression system for the production of edible vaccines. A synthetic cholera toxin B subunit gene (CTB) was fused with a synthetic neutralizing epitope gene of the porcine epidemic diarrhea virus (sCTB-sCOE), and the sCTB-sCOE fusion gene was introduced into a plant expression vector under the control of the ubiquitin promoter. This plant expression vector was transformed into lettuce (Lactuca sativa L.) using the Agrobacterium-mediated transformation method. Stable integration and transcriptional expression of the sCTB-sCOE fusion gene was confirmed using genomic DNA PCR analysis and northern blot analysis, respectively. The results of western blot analysis with anti-cholera toxin and anti-COE antibody showed the synthesis and assembly of CTB-COE fusion protein into oligomeric structures with pentameric sizing. The biological activity of CTB-COE fusion protein to its receptor, G(M1)-ganglioside, in transgenic plants was confirmed via G(M1)-ELISA with anti-cholera toxin and anti-COE antibody. Based on G(M1)-ELISA, the expression level of CTB-COE fusion proteins reached 0.0065% of the total soluble protein in transgenic lettuce leaf tissues. Transgenic lettuce successfully expressing CTB-COE fusion protein will be tested to induce efficient immune responses against porcine epidemic diarrhea virus infection by administration with raw material.

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Section: Introductionmentioning

confidence: 99%

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

2010

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“…Cholera toxin B subunit (CTB) has been shown to be non-toxic and immunogenic in humans, and has been successfully used as an adjuvant in oral and intranasal vaccinations to elicit Th2-mediated mucosal immunity. 26 In order to stimulate mucosal Th1 responses, adjuvants such as cytosine guanine oligodeoxynucleotides (CpG-ODNs) have been proven effective. 26 CpG-ODNs are synthetic oligodeoxynucleotides containing immunostimulatory CpG motifs that have been shown to predominantly induce Th1-polarizing cytokines.…”

Section: Vaccine Candidatesmentioning

confidence: 99%

“…26 In order to stimulate mucosal Th1 responses, adjuvants such as cytosine guanine oligodeoxynucleotides (CpG-ODNs) have been proven effective. 26 CpG-ODNs are synthetic oligodeoxynucleotides containing immunostimulatory CpG motifs that have been shown to predominantly induce Th1-polarizing cytokines. 27 Therefore, the adjuvant not only acts as an immunopotentiator but can also determine the type of immune response elicited.…”

Section: Vaccine Candidatesmentioning

confidence: 99%

The future for vaccine development againstEntamoeba histolytica

Quach

St‐Pierre

Chadee

2014

Human Vaccines & Immunotherapeutics

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“…Adjuvanzien, wie beispielsweise bakterielle Enterotoxine [18] verstärken die Immunreaktion auf Antigene und könnten möglicherweise auch allergische Reaktionen verstärken.…”

Section: Bioinformatische (In Silico) Analysenunclassified

Allergenitätsbewertung von Lebensmitteln aus gentechnisch veränderten Pflanzen

Schauzu

Pöting

Rubin

et al. 2012

Bundesgesundheitsbl.

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The placing on the European Union's market of genetically modified crops requires authorization by the European Commission which is based on the proof that the derived foods are as safe as their conventional counterparts. The assessment of potential allergenicity is part of the necessary investigations recommended in the updated Guidance Document of the Scientific Panel on Genetically Modified Organisms (GMO) of the European Food Safety Authority (EFSA), which is based on internationally agreed recommendations. All genetically modified crops which so far have been authorized in the European Union were evaluated by the EFSA GMO Panel which considered it unlikely that their overall allergenicity has been altered.

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Mucosal adjuvants and anti-infection and anti-immunopathology vaccines based on cholera toxin, cholera toxin B subunit and CpG DNA

Cited by 169 publications

References 35 publications

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

The future for vaccine development againstEntamoeba histolytica

Allergenitätsbewertung von Lebensmitteln aus gentechnisch veränderten Pflanzen

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