Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination

Hinc, Krzysztof; Stasiłojć, Małgorzata; Piątek, Iwona; Peszyńska-Sularz, Grażyna; Isticato, Rachele; Ricca, Ezio; Obuchowski, Michał; Iwanicki, Adam

doi:10.1371/journal.pone.0095187

Cited by 15 publications

(30 citation statements)

References 47 publications

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“…To check how the choice of anchoring protein influences interaction of the recombinant spores with the immune system, we performed mucosal immunization of mice with all variants of the FliD-presenting spores. Since results of our previous research indicated that the recombinant spores did not elicit immune response in mice upon oral immunization without the use of appropriate adjuvants (Hinc et al, 2014;Stasiłojć et al, 2015), we decided to administrate spores by the intranasal route, which was shown to be compatible with such antigen delivery systems (Huang et al, 2010;. We were not able to detect a statistically significant increase in levels of FliD-specific antibodies in sera, lungs or gastrointestinal tracts of immunized animals (data not shown).…”

Section: Immune Response To Recombinant Sporesmentioning

confidence: 91%

“…(Negri et al, 2013) BAN02 amyE::cotC-fliD (Negri et al, 2013) BAN03 amyE::cotG-fliD (Negri et al, 2013) BAN04 amyE::cotZ-fliD (Negri et al, 2013) Indirect ELISA for detection of antigen-specific antibodies. FliD-specific antibodies in saponin extracts of gastrointestinal tracts or lungs, and sera of the immunized animals were detected as previously described (Hinc et al, 2014). Briefly, plates were coated with 100 ml per well of the specific antigen (2 mg/ml in carbonate/bicarbonate buffer) and left at room temperature overnight.…”

Section: Methodsmentioning

confidence: 99%

“…Isolation of splenocytes. The mice were sacrificed and their spleens were isolated as previously described (Hinc et al, 2014). Briefly, the mice were sacrificed and spleens were aseptically removed.…”

Section: Methodsmentioning

confidence: 99%

“…In most of such studies, three spore coat proteins, CotB, CotC and CotG, have been used for anchoring of heterologous proteins. It has been also shown that the CotZ and CgeA crust proteins can be also useful for spore display technology (Hinc et al, 2014;Iwanicki et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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The choice of anchoring protein influences interaction of recombinant Bacillus spores with the immune system

Piekarska¹,

Pełka²,

Peszyńska-Sularz³

et al. 2017

Acta Biochim Pol

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The technology of display of heterologous proteins on the surface of Bacillus subtilis spores enables use of these structures as carriers of antigens for mucosal vaccination. Currently, there are no technical possibilities to predict whether a designed fusion will be efficiently displayed on the spore surface and how such recombinant spores will interact with cells of the immune system. In this study, we compared four variants of B. subtilis spores presenting a fragment of a FliD protein from Clostridium difficile in fusion with CotB, CotC, CotG or CotZ spore coat proteins. We show that these spores promote their own phagocytosis and activate both, the J774 macrophages and JAWSII dendritic cells of murine cell lines. Moreover, we used these spores for mucosal immunization of mice. We conclude that the observed effects vary with the type of displayed FliD-spore coat protein fusion and seem to be mostly independent of its abundance and localization in the spore coat structure.

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Section: Immune Response To Recombinant Sporesmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The choice of anchoring protein influences interaction of recombinant Bacillus spores with the immune system

Piekarska¹,

Pełka²,

Peszyńska-Sularz³

et al. 2017

Acta Biochim Pol

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“…Major efforts have been devoted to the development of new mucosal vaccination strategies based on adjuvants able to induce sIgA or on novel delivery systems based on synthetic nanoparticles, viral particles, microbial cells or bacterial spores [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC

et al. 2020

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Background: Spore-forming bacteria of the Bacillus genus are widely used probiotics known to exert their beneficial effects also through the stimulation of the host immune response. The oral delivery of B. toyonensis spores has been shown to improve the immune response to a parenterally administered viral antigen in mice, suggesting that probiotics may increase the efficiency of systemic vaccines. We used the C fragment of the tetanus toxin (TTFC) as a model antigen to evaluate whether a treatment with B. toyonensis spores affected the immune response to a mucosal antigen. Results: Purified TTFC was given to mice by the nasal route either as a free protein or adsorbed to B. subtilis spores, a mucosal vaccine delivery system proved effective with several antigens, including TTFC. Spore adsorption was extremely efficient and TTFC was shown to be exposed on the spore surface. Spore-adsorbed TTFC was more efficient than the free antigen in inducing an immune response and the probiotic treatment improved the response, increasing the production of TTFC-specific secretory immunoglobin A (sIgA) and causing a faster production of serum IgG. The analysis of the induced cytokines indicated that also the cellular immune response was increased by the probiotic treatment. A 16S RNA-based analysis of the gut microbial composition did not show dramatic differences due to the probiotic treatment. However, the abundance of members of the Ruminiclostridium 6 genus was found to correlate with the increased immune response of animals immunized with the spore-adsorbed antigen and treated with the probiotic. Conclusion: Our results indicate that B. toyonensis spores significantly contribute to the humoral and cellular responses elicited by a mucosal immunization with spore-adsorbed TTFC, pointing to the probiotic treatment as an alternative to the use of adjuvants for mucosal vaccinations.

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Recent progress in Bacillus subtilis spore-surface display: concept, progress, and future

Wang

Yang

2017

Appl Microbiol Biotechnol

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With the increased knowledge on spore structure and advances in biotechnology engineering, the newly developed spore-surface display system confers several inherent advantages over other microbial cell-surface display systems including enhanced stability and high safety. Bacillus subtilis is the most commonly used Bacillus species for spore-surface display. The expression of heterologous antigen or protein on the surface of B. subtilis spores has now been practiced for over a decade with noteworthy success. As an update and supplement to other previous reviews, we comprehensively summarize recent studies in the B. subtilis spore-surface display technique. We focus on its benefits as well as the critical factors affecting its display efficiency and offer suggestions for the future success of this field.

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Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination

Cited by 15 publications

References 47 publications

The choice of anchoring protein influences interaction of recombinant Bacillus spores with the immune system

The choice of anchoring protein influences interaction of recombinant Bacillus spores with the immune system

A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC

Recent progress in Bacillus subtilis spore-surface display: concept, progress, and future

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