Mucor circinelloides whole-cells as a biocatalyst for the production of ethyl esters based on babassu oil

Abstract

The intracellular lipase production by Mucor circinelloides URM 4182 was investigated through a step-by-step strategy to attain immobilized whole-cells with high lipase activity. Physicochemical parameters, such as carbon and nitrogen sources, inoculum size and aeration, were studied to determine the optimum conditions for both lipase production and immobilization in polyurethane support. Olive oil and soybean peptone were found to be the best carbon and nitrogen sources, respectively, to enhance the intracellular lipase activity. Low inoculum level and poor aeration rate also provided suitable conditions to attain high lipase activity (64.8 ± 0.8 U g(-1)). The transesterification activity of the immobilized whole- cells was assayed and optimal reaction conditions for the ethanolysis of babassu oil were determined by experimental design. Statistical analysis showed that M. circinelloides whole-cells were able to produce ethyl esters at all tested conditions, with the highest yield attained (98.1 %) at 35 °C using an 1:6 oil-to-ethanol molar ratio. The biocatalyst operational stability was also assayed in a continuous packed bed reactor (PBR) charged with glutaraldehyde (GA) and Aliquat-treated cells revealing half-life of 43.0 ± 0.5 and 20.0 ± 0.8 days, respectively. These results indicate the potential of immobilized M. circinelloides URM 4182 whole-cells as a low-cost alternative to conventional biocatalysts in the production of ethyl esters from babassu oil.

Isolation, Screening and Identification of Lipase Producing Fungi from Oil Contaminated Soil of Shani Mandir Ujjain

“…In this regard, a good number of fungi have been screened for lipase production. Molds are best microbial population for lipase production commercially because these are easily extracted from fermentation processes, safe and easy handling, in addition to the growing potential of the same as the use for the said purposes, in the form of whole cell biocatalysts (Alberton et al, 2010;Andrade et al, 2014). Present study also supports this fact because all isolated fungi are filamentous in nature (Photo Plate 3 and 4).…”

Synthesis, Characterization and Application of a Polyurethane-Based Support for Immobilizing Membrane-Bound Lipase

“…The obtained PUFs were left at room temperature for 24 h for a complete cure before being cut to specific dimensions for the assays to be carried out. All formulations are shown in Table 1, which were selected in agreement with the technical expertise of Evonik ® aiming at preparing flexible foams with similar properties to those used in previous works (Andrade et al, 2012;Andrade et al, 2014).…”

“…This filamentous fungus was selected based on earlier studies in which it was considered to be effective membranebound lipase producer that grows well in triacylglycerol-containing culture media with high transesterification yield (Andrade et al, 2012;Andrade et al, 2014). All PUFs were synthesized aiming at evaluating the influence of rotation speed and type of polyol to be used in each formulation.…”

“…The catalytic performance of the biocatalyst was assessed in both hydrolysis and transesterification reactions. Babassu oil was chosen as feedstock in the transesterification reactions so as to enable a comparison with previous works (Andrade et al, 2012;2014).…”

Isolamento e seleção de fungos produtores de lipases com base na atividade lipásica e no potencial hidrolítico sobre óleo comestível de soja e escuma de caixa de gordura

“…Os fungos, dentre os microrganismos, são fontes preferenciais de lipases para uso comercial, pois essas são geralmente extracelulares, facilitando a sua extração do meio fermentado; também por serem eles considerados microrganismos seguros para a manipulação, além da expectativa crescente do seu emprego como biocatalizadores na forma de células integrais lipásicas, imobilizadas ou não, simplificando e reduzindo o custo dos processos reacionais nos quais são utilizados (RODRIGUES & FERNANDEZ-LAFUENTE, 2010;SINGH & MUKHOPADHYAY, 2012;ANDRADE et al, 2014;CARVALHO et. al., 2015).…”