Mucopolysaccharides from chicken skin of three age groups

Complex Saccharide Metabolism in Cystic Fibrosis Fibroblasts

“…Polysaccharides were separated by ion exchange chromatography (l3), and by electrophoresis on cellulose acetate strips using a Beckman model carried out in 0.1 M barium acetate, pH 8 (28) or in 0.1 M pyridine-0.566 M formic acid buffer pH 3.0 (15). A constant voltage of 140 V giving a current of approximately 0.5 mA/cm was used for 45 min.…”

Molecular basis of the adhesion ofSalmonellaserotypes to chicken muscle fascia

“…For this reason the role of matrix GAGs in adhesion of Salmonella to connective tissue of poultry carcasses was examined. Hyaluronan [the terms hyaluronic acid and hyaluronate are now reserved to specify the acid and dissociated forms respectively (Balazs et al, 1986)] and chondroitin sulphate were chosen as candidate GAGs because they are present in chicken skin (Kondo et al, 1971) and are the major GAG components of the matrix of connective tissue in man (Davies & Davies, 1962). In corroboration of the work of Campbell (1984), who demonstrated that crude preparations of chicken comb hyaluronan could inhibit adhesion of Salmonella and E. coli strains to muscle fascia when added to bacterial suspensions at concentrations of 01 and 1 mg-ml" 1 , in the current work it has been shown qualitatively that pre-treatment of bacteria with hyaluronan, but not with a b d e chondroitin sulphate, can reduce the numbers of cells which adhere to fascia (Figs 1,2).…”

The Biological Role of Hyaluronic Acid

“…Upon a damage, say a bone fracture, these cells start 'repairing' by reproducing the initial embryonic processes of the bone formation from the cartilage models. In both cases the maximum intensity of metabolism is observed at the beginning of ontogenesis, especially during histo-and organogenesis [61,64]. Then the cartilaginous tissue is replaced by bone tissue with the aid of the osteoblasts and the osteoclasts [45].…”