Mucoid Strains of Pseudomonas Aeruginosa: The Influence of Culture Medium on the Stability of Mucus Production

Transfer of a chromosomal locus responsible for mucoid colony morphology in Pseudomonas aeruginosa isolated from cystic fibrosis patients to P. aeruginosa PAO

“…A reported feature of mucoid strains is their instability during culture (Zierdt and Schmidt, 1964;Govan, 1975;Fyfe and Govan, 1983). Govan (1 975) reported that the use of desoxycholate citrate agar allows maintenance of the mucoid colony morphology.…”

Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung

“…Bacterial colonization is probably promoted by the unique composition of the sputum, which contains mucin, lipids, proteins, amino acids, ions and DNA released by neutrophils in concentrations above the mean of a healthy individual (Barth & Pitt, 1996;Kilbourn, 1978;Sahu & Lynn 1978;Smith et al, 1988). Indeed, a number of studies have provided evidence for a correlation between individual sputum components and the emergence or stabilization of certain P. aeruginosa pheno-types (Govan, 1975;Ohman & Chakrabarty, 1982;Wang et al, 1996;Yoon et al, 2002).…”

The high amino-acid content of sputum from cystic fibrosis patients promotes growth of auxotrophic Pseudomonas aeruginosa

“…The airways of CF patients are invariably colonised by Pseudomonas aeruginosa which, during the course of the disease, undergoes many phenotypic variations. Production of alginate exopolysaccharide [2], sensitivity to human serum [3], hypersensitivity to semi-synthetic penicillins [4] and other phenotypic alterations appear to be a result of the adaptation of the bacterium to the environment in the CF lung. This adaptability extends to the nutritional status of the organism with many strains of E!…”