Mucocutaneous leishmaniasis: accuracy and molecular validation of noninvasive procedures in a L. (V.) braziliensis–endemic area

Gomes, Ciro Martins; Paula, Natália Aparecida de; Cesetti, Mariana Vicente; Roselino, Ana Maria Ferreira; Sampaio, Raimunda Nonata Ribeiro

doi:10.1016/j.diagmicrobio.2014.05.002

Cited by 22 publications

(33 citation statements)

References 33 publications

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“…A composite reference standard (CRS) was defined. An ATL case was defined by detection of the parasite in at least one of the following examinations: histopathology, culture, smears, and conventional PCR of biopsy or swab samples, as described elsewhere (8). In the absence of parasite detection, ATL was diagnosed based on the MST or IIF positivity in combination with the presence of granulomas on histopathologic examination and a lack of identification of another etiological agent by special staining methods.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the combination of swabs with qPCR is emerging as a powerful diagnostic tool because of its noninvasive yet simple collection method. medium), smears from the lesion, conventional PCR of biopsy and swab samples, as previously described (8,31), and qPCR of biopsy and swab samples.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, serological techniques may yield widely varied accuracy values (3). In contrast, although not completely sensitive, PCR can improve the accuracy of ATL diagnosis (5,8,9). Strategies that might improve the sensitivity of PCR include the use of reliable DNA isolation methods, primer pairs that amplify the kinetoplast DNA (kDNA) sequences of Leishmania, and real-time PCR (qPCR) techniques (5,10,11).…”

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confidence: 99%

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Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

et al. 2017

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The precise diagnosis of American tegumentary leishmaniasis (ATL) is an essential task due to the disease's associated morbidity. A noninvasive, extremely sensitive, and highly specific exam is critical, particularly for mucosal leishmaniasis (ML), in which a low parasite quantity is expected. We aimed to compare the diagnostic accuracy of swab and biopsy sample analysis using SYBR Green-and TaqManbased real-time PCR (qPCR) assays with that of a composite reference standard consisting of the Montenegro skin test, serology, histopathology, smears, culture, and conventional PCR. In total, 55 patients with ATL (ML, 18 patients; cutaneous leishmaniasis [CL], 37 patients) and 36 patients without ATL were studied. qPCR analysis of swabs was more accurate when using SYBR Green (87.88%; 95% confidence interval [CI], 77.86 to 93.73 patients) than when using TaqMan (78.79%; 95% CI, 67.49 to 86.92%) (P ϭ 0.031). SYBR Green (84.72%; 95% CI, 74.68 to 91.25%) was also more accurate than TaqMan (73.61%; 95% CI, 62.42 to 82.41%) for biopsy samples (P ϭ 0.008). All qPCR methods were 100% specific. Swabs and biopsy specimens had similar sensitivity when using the same chemistry (P ϭ 0.125 for SYBR Green and P ϭ 0.625 for TaqMan). Moreover, qPCR achieved better performance than most existing techniques used for the diagnosis of ATL and also detected the Leishmania parasite in a greater proportion of patients than the associated histopathology, smear, culture, and conventional PCR techniques did. Swabs therefore represent a useful diagnostic tool because they not only are noninvasive but also can achieve an accuracy similar to that of biopsy samples. The high accuracy of SYBR Green-based qPCR may also reduce the requirement for associated parasitological tests for ATL diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

et al. 2017

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“…In the Americas, the main species that causes ATL is L. (V.) braziliensis 8 . Previous studies of Leishmania serology, Montenegro skin tests, and the presence of Leishmania DNA identified using PCR techniques have shown that in Brazilian endemic areas, 10% of the population has subclinical infections 7 .…”

Section: Discussionmentioning

confidence: 99%

“…Only 6 months later and after the third biopsy, the histopathology showed a lymphohistiocytic infiltrate with granulomas and multinucleated cells, and some rare roundish forms compatible with amastigotes, which were confirmed by immunohistochemistry. A skin fragment tested positive for Leishmania culture, and the parasite was identified as L. (V.) braziliensis using a polymerase chain reaction (PCR) assay and restriction enzyme analysis 8 . Montenegro skin test and indirect immunofluorescence assay results were positive and human immunodeficiency virus (HIV) serology test results were negative.…”

Section: Case Reportmentioning

confidence: 99%

American cutaneous leishmaniasis triggered by electrocoagulation

Martins

Santos

Lima

et al. 2018

Rev. Soc. Bras. Med. Trop.

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Cutaneous leishmaniasis is usually transmitted by infected phlebotomine sand fly bites that initiate local cutaneous lesions. Few reports in the literature describe other modes of transmission. We report a case of a previously healthy 59-year-old woman who underwent electrocoagulation to remove seborrheic keratosis confirmed by dermatoscopy. Three months later, a skin fragment tested positive for Leishmania culture; the parasite was identified as L. (V.) braziliensis. Trauma may generate inflammatory cascades that favor Leishmania growth and lesion formation in previously infected patients. American cutaneous leishmaniasis is a dynamic disease with unclear pathophysiology because of continually changing environments, demographics, and human behaviors.

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Bioinformatics in Leishmania Drug Design

Gupta

Dandekar

2018

Drug Resistance in Leishmania Parasites

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Mucocutaneous leishmaniasis: accuracy and molecular validation of noninvasive procedures in a L. (V.) braziliensis–endemic area

Cited by 22 publications

References 33 publications

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

American cutaneous leishmaniasis triggered by electrocoagulation

Bioinformatics in Leishmania Drug Design

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