Mucoadhesive nanoparticles-based oral drug delivery systems enhance ameliorative effects of low molecular weight heparin on experimental colitis

Yan, Yan; Ying, Sun; Wang, Pengchong; Zhang, Rui; Huo, Chuanchuan; Gao, Tingting; Song, Chenghua; Xing, Jianfeng; Dong, Yalin

doi:10.1016/j.carbpol.2020.116660

Cited by 42 publications

(21 citation statements)

References 38 publications

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“…The four in vivo studies showed no obvious toxicity, no pathological changes and no difference in hematological or biochemical parameters from the control group, indicating high level of safety when nanoparticles were administrated intranasally, orally or intramuscularly to mice, rats and chickens ( Liu et al, 2015 ; Jin et al, 2017 ; Zhao et al, 2017 ; Yan et al, 2020 ). In one of the studies, TMC nanoparticles loaded with low molecular weight heparin (LMWH) reversed a drug-induced colitis in mice when the mice were treated orally for 15 days, while mice treated with free LMWH showed no signs of recovery ( Yan et al, 2020 ).…”

Section: Results Of Cytotoxicity Measurements On Chitosan Containing ...mentioning

confidence: 93%

“…Seven papers that investigated different quaternized chitosan nanoparticles and their cytotoxicity were identified, four with in vivo studies ( Liu et al, 2015 ; Jin et al, 2017 ; Zhao et al, 2017 ; Yan et al, 2020 ), two with both ex vivo and in vitro studies ( Amidi et al, 2006 ; Yin et al, 2009 ) and one in vitro study using three different cell lines ( Facchinatto et al, 2021 ). An overview of cytotoxicity studies concerning nanoparticles with quaternized chitosan is seen in Table 6 .…”

Section: Results Of Cytotoxicity Measurements On Chitosan Containing ...mentioning

confidence: 99%

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The Potential of Chitosan in Nanomedicine: An Overview of the Cytotoxicity of Chitosan Based Nanoparticles

et al. 2022

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The unique properties and applications of nanotechnology in targeting drug delivery, cosmetics, fabrics, water treatment and food packaging have received increased focus the last two decades. The application of nanoparticles in medicine is rapidly evolving, requiring careful investigation of toxicity before clinical use. Chitosan, a derivative of the natural polysaccharide chitin, has become increasingly relevant in modern medicine because of its unique properties as a nanoparticle. Chitosan is already widely used as a food additive and in food packaging, bandages and wound dressings. Thus, with an increasing application worldwide, cytotoxicity assessment of nanoparticles prepared from chitosan is of great interest. The purpose of this review is to provide an updated status of cytotoxicity studies scrutinizing the safety of chitosan nanoparticles used in biomedical research. A search in Ovid Medline from 23 March 1998 to 4 January 2022, with the combination of the search words Chitosan or chitosan, nanoparticle or nano particle or nanosphere or nanocapsule or nano capsule, toxicology or toxic or cytotoxic and mucosa or mucous membrane resulted in a total of 88 articles. After reviewing all the articles, those involving non-organic nanoparticles and cytotoxicity assays conducted exclusively on nanoparticles with anti-tumor effect (i.e., having cytotoxic effect) were excluded, resulting in 70 articles. Overall, the chitosan nanoparticles included in this review seem to express low cytotoxicity regardless of particle composition or cytotoxicity assay and cell line used for testing. Nonetheless, all new chitosan derivatives and compositions are recommended to undergo careful characterization and cytotoxicity assessment before being implemented on the market.

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Section: Results Of Cytotoxicity Measurements On Chitosan Containing ...mentioning

confidence: 93%

Section: Results Of Cytotoxicity Measurements On Chitosan Containing ...mentioning

confidence: 99%

The Potential of Chitosan in Nanomedicine: An Overview of the Cytotoxicity of Chitosan Based Nanoparticles

et al. 2022

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“…An oral NP system with zein as the carrier is widely used in the treatment of UC. ,, Zein, an excellent natural carrier, contains hydrophobic amino acids to encapsulate the hydrophobic chemical compound, but the stability of the nanoparticles needs to be improved. CS, which is a natural anionic polysaccharide, is used as a stabilizer to protect the NPs due to its negative charge .…”

Section: Resultsmentioning

confidence: 99%

Oral Core–Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy

Wang

Zhang

et al. 2021

ACS Appl. Mater. Interfaces

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Although magnolol (Mag), an anti-inflammatory natural compound, has been demonstrated to play protective effects on ulcerative colitis (UC), its application as an alternative therapeutic reagent for UC treatment is still greatly impeded due to its poor stability in the gastrointestinal tract and insufficient accumulation in the inflamed colon lesion. Nano-/microsized drug delivery systems can potentially overcome some challenges regarding the oral administration of phytochemicals, which still confront premature early drug release, degradation of NPs, or the sustained drug release of MPs. In this study, we primarily loaded Mag into the core–shell zein-based nanoparticles with chondroitin sulfate coating (Mag@CS-Zein NPs) with an average size of 142.27 ± 5.11 nm, showing significant macrophage-targeting and enhanced colon epithelial cellular uptake capacity. Then, we embedded Mag@CS-Zein NPs into hydrogel microspheres via an electrospraying technology. The Mag@CS-Zein NPsinMPs presented a uniform-sized sphere with an average size of 164.36 ± 6.29 μm and sustained drug-release profiles. Compared to CS-Zein NPs, the developed CS-Zein NPsinMPs exhibited prolonged colon retention on the inflammatory surface, as seen from ex vivo and in vivo imaging fluorescence adhesion experiments. Based on the advantage of the combination of hybrid nanoparticles-in-microparticles, oral administration of Mag@CS-Zein NPsinMPs significantly alleviated colitis symptoms in DSS-treated mice by regulating the expression levels of proinflammatory cytokines (TNF-α, IL-6, and IL-1β) and anti-inflammatory cytokines (IL-10) and factor accelerated colonic mucosal barrier repair via upregulating the expression of ZO-1 and occludin. This study provides great insights into the oral drug delivery of natural compounds for UC therapy.

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“…Polymer-and lipid-based nanocarriers, such as polymeric micelles, polymeric nanoparticles, lipid nanocapsules, microemulsions, and solid lipid nanoparticles, have been investigated to improve the HP oral absorption [13][14][15][16][17]. Chitosan and alginate biopolymers have received significant attention in this context because of their non-toxic, biocompatible, and biodegradable properties [18,19].…”

Section: Introductionmentioning

confidence: 99%

Chitosan/Alginate Nanoparticles for the Enhanced Oral Antithrombotic Activity of Clam Heparinoid from the Clam Coelomactra antiquata

et al. 2022

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Chitosan/alginate nanoparticles (DG1-NPs and DG1/Cur-NPs) aiming to enhance the oral antithrombotic activity of clam heparinoid DG1 were prepared by ionotropic pre-gelation. The influence of parameters, such as the concentration of sodium alginate (SA), chitosan (CTS), CaCl2, clam heparinoid DG1, and curcumin (Cur), on the characteristics of the nanoparticles, were investigated. Results indicate that chitosan and alginate can be used as polymer matrices to encapsulate DG1, and nanoparticle characteristics depend on the preparation parameters. Nano-particles should be prepared using 0.6 mg/mL SA, 0.33 mg/mL CaCl2, 0.6 mg/mL CTS, 7.2 mg/mL DG1, and 0.24 mg/mL Cur under vigorous stirring to produce DG1-NPS and DG1/Cur-NPS with small size, high encapsulation efficiency, high loading capacity, and negative zeta potential from approximately −20 to 30 mV. Data from scanning electron microscopy, Fourier-transform infrared spectrometry, and differential scanning calorimetry analyses showed no chemical reaction between DG1, Cur, and the polymers; only physical mixing. Moreover, the drug was loaded in the amorphous phase within the nanoparticle matrix. In the acute pulmonary embolism murine model, DG1-NPs enhanced the oral antithrombotic activity of DG1, but DG1/Cur-NPs did not exhibit higher antithrombotic activity than DG1-NPs. Therefore, the chitosan/alginate nanoparticles enhanced the oral antithrombotic activity of DG1, but curcumin did not further enhance this effect.

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Mucoadhesive nanoparticles-based oral drug delivery systems enhance ameliorative effects of low molecular weight heparin on experimental colitis

Cited by 42 publications

References 38 publications

The Potential of Chitosan in Nanomedicine: An Overview of the Cytotoxicity of Chitosan Based Nanoparticles

The Potential of Chitosan in Nanomedicine: An Overview of the Cytotoxicity of Chitosan Based Nanoparticles

Oral Core–Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy

Chitosan/Alginate Nanoparticles for the Enhanced Oral Antithrombotic Activity of Clam Heparinoid from the Clam Coelomactra antiquata

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