Mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon

Kumar, Vijay; Mahajan, Neha; Kaur, Jasleen; Devi, Kamalini; Dharavath, Ravinder Naik; Singh, Ravindra; Kondepudi, Kanthi Kiran; Bishnoi, Mahendra

doi:10.1016/j.biopha.2021.112452

Cited by 22 publications

(32 citation statements)

References 66 publications

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“…They demonstrated that subcutaneous administration of CPZ antagonizes the TRPV1 channel expressed on sensory neurons and reduces the PAR-2 mediated gastric mucus secretion [ 16 ]. In our previous studies, we also found that capsaicin induced activation of TRPV1 promotes mucus secretion in the gut and resiniferatoxin-mediated chemodenervation of the TRPV1 expressing sensory neurons severely impairs gut mucus production and promotes gut dysbiosis [ 18 , 37 ] Moreover, Kistner et al demonstrated that CPZ rectal administration desensitizes TRPA1/TRPV1 expressing sensory neurons [ 12 ]. With support of available literature, we argue that CPZ-induced alterations in colonic mucosal health will be mediated through TRPV1 and TRPV1 channels.…”

Section: Discussionmentioning

confidence: 99%

Intrarectal Capsazepine Administration Modulates Colonic Mucosal Health in Mice

Kumar

Devi

et al. 2022

IJMS

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Antagonism of transient receptor potential vanniloid-1 (TRPV1) and desensitization of transient receptor potential ankyrin-1 (TRPA1) nociceptors alleviate inflammatory bowel diseases (IBD)-associated chronic pain. However, there is limited literature available about their role in regulating the mucosal layer, its interaction with host physiology, and luminal microbial community. The present study focuses on the effects’ intra rectal administration of capsazepine (modulator of TRPA1/TRPV1 expressing peptidergic sensory neurons) on colonic mucus production and gut health. We performed histological analysis, gut permeability alteration, gene expression changes, metabolite profiling, and gut microbial abundance in the ileum, colon, and cecum content of these animals. Intra rectal administration of capsazepine modulates TRPA1/TRPV1-positive nociceptors (behavioral pain assays) and resulted in damaged mucosal lining, increased gut permeability, and altered transcriptional profile of genes for goblet cell markers, mucus regulation, immune response, and tight junction proteins. The damage to mucosal lining prevented its role in enterosyne (short chain fatty acids) actions. These results suggest that caution must be exercised before employing TRPA1/TRPV1 modulation as a therapeutic option to alleviate pain caused due to IBD.

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Section: Discussionmentioning

confidence: 99%

Intrarectal Capsazepine Administration Modulates Colonic Mucosal Health in Mice

Kumar

Devi

et al. 2022

IJMS

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“…In a 12-week study with mice kept on normal chow or HFD (32% animal lard, corresponding to 60% energy from fat), capsaicin (2 mg/kg/day, per os ) reduced weight gain by approximately 50% in the HDF group ( Figure 1 A,B), but had no effect on the body weight in animals that consumed normal chow ( Figure 1 A) [ 12 ]. Furthermore in rats, dietary capsaicin (0.014–0.028%) suppressed visceral (for example, perirenal) fat accumulation in a dose-dependent manner [ 13 ].…”

Section: Dietary Capsaicin In Animal Experimentsmentioning

confidence: 99%

“…The GI tract is densely innervated by capsaicin-sensitive (TRPV1-expressing) nerves that sense visceral pain (afferent function) and regulate intestinal motility (efferent function) [ 67 , 68 ]. In rats, dietary capsaicin stimulates mucus production in the colon that, in turn, may reduce fat absorption [ 12 ]. In men, capsaicin hastens the intestinal transit of the meal [ 69 , 70 ], although it has no effect on gastric emptying [ 71 ].…”

Section: Dietary Capsaicin: Mechanisms Of Actionmentioning

confidence: 99%

“…In the C57BL/6J mouse, capsaicin (2 mg per os ) was reported to increase the contribution of Akkermansia to the gut microbiota, presumably by stimulating mucin production in the colon [ 12 ]. In this context, it may be relevant that mucin-producing columnar epithelium in the colon expresses TRPV1 [ 109 ].…”

Section: Capsaicin and Gut Microbiotamentioning

confidence: 99%

“… In C57BL/6 mice, capsaicin (2 mg/kg/day, per os ) reduced weight gain by approximately 50% in animals that were kept on HFD ( A , B ), but had no effect on body weight in the normal chow group ( B ). Figure courtesy of Dr. Mahendra Bishnoi (based on [ 12 ]). ** p < 0.01 versus control animals, ## p < 0.01 versus HFD animals.…”

Section: Figurementioning

confidence: 99%

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Capsaicin for Weight Control: “Exercise in a Pill” (or Just Another Fad)?

Szállaśi

2022

Pharmaceuticals

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Medical management of obesity represents a large unmet clinical need. Animal experiments suggest a therapeutic potential for dietary capsaicin, the pungent ingredient in hot chili peppers, to lose weight. This is an attractive theory since capsaicin has been a culinary staple for thousands of years and is generally deemed safe when consumed in hedonically acceptable, restaurant-like doses. This review critically evaluates the available experimental and clinical evidence for and against capsaicin as a weight control agent and comes to the conclusion that capsaicin is not a magic “exercise in a pill”, although there is emerging evidence that it may help restore a healthy gut microbiota.

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Oleuropein Prevents OVA‐Induced Food Allergy in Mice by Enhancing the Intestinal Epithelial Barrier and Remodeling the Intestinal Flora

Guo

et al. 2022

Molecular Nutrition Food Res

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Scope This study assesses whether oleuropein prevents ovalbumin (OVA)‐induced food allergy (FA) and investigates the underlying mechanisms. Methods and results A Balb/c FA mouse model is established and maintained for 7 weeks. The subjects are administered OVA by oral gavage to induce FA and supplemented with different oleuropein doses (1.00–20.00 mg kg−1 per day) to evaluate its preventative efficacy. The results indicate that oleuropein effectively alleviates OVA‐induced allergy symptoms and promotes temperature elevation in sensitized mice. The secretion of serology‐specific OVA‐immunoglobulin (Ig)E, OVA‐IgG, and histamine is inhibited in the sensitized mice. Oleuropein dramatically upregulates the expression of intestinal tight junction (TJ) proteins, regenerating gene (Reg) IIIγ, and interleukin (IL)‐22, enhancing the physical and biochemical barrier function of the intestinal epithelium. Additionally, oleuropein improves the immune homeostasis of the intestinal epithelium by affecting the function of mucosal mast cells and regulatory T (Treg) cells. The disordered intestinal flora of the sensitized mice also improves after oleuropein administration. Conclusions These findings suggest that oleuropein prevents FA by enhancing intestinal epithelial barrier function and improving immune homeostasis and intestinal flora in sensitized mice. Therefore, diets rich in oleuropein should be recommended for people with FA.

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Mucin secretory action of capsaicin prevents high fat diet-induced gut barrier dysfunction in C57BL/6 mice colon

Cited by 22 publications

References 66 publications

Intrarectal Capsazepine Administration Modulates Colonic Mucosal Health in Mice

Intrarectal Capsazepine Administration Modulates Colonic Mucosal Health in Mice

Capsaicin for Weight Control: “Exercise in a Pill” (or Just Another Fad)?

Oleuropein Prevents OVA‐Induced Food Allergy in Mice by Enhancing the Intestinal Epithelial Barrier and Remodeling the Intestinal Flora

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