Mucin secretion in inflammatory bowel disease: Comparison of a macrophage-derived mucin secretagogue (MMS-68) to conventional secretagogues

Abstract

We have described a novel macrophage-derived tnucin secretagogue (MMS-68) that mediates mucin secretion in colon cancer cell lines and explants of normal and inflammatory bowel disease (IBD) mucosa. we compared MMS-68 induced mucin release with other known intestinal mucin secretagogues in normal colon explants and in the HT-29 colon cancer cell line, and to study the effects of MMS-68 on mucin release from inflamed and uninflamed ulcerative colitis (UC) and Crohn's disease (CD) mucosa. In normal colonic explants and HT-29 cells, each of the secretagogues including MMS-68-induced mucin release two-to fivefold more than culture medium alone. I n HT-29 cells, MMS-68 plus leukotriene C, (LTC,) induced ;I 50% increase in mucin release over either secretagoguc alone, and MMS-68 plus platelet-activating factor (PAF) markedly enhanced mucin release by eightfold over either secretagogue. In colonic explants from patients with UC

The Role of Arachidonic Acid Regulatory Enzymes in Colorectal Disease

“…[121][122][123][124] PAF has been shown to regulate mucin secretion in colonic epithelial cells. 125 The role of PAF in the malignant transformation of epithelial cells is unknown.…”