Mucin Granule Intraluminal Organization in Living Mucous/Goblet Cells

Pérez-Vilar, Juan; Mabolo, Raean; McVaugh, Cheryl T.; Bertozzi, Carolyn R.; Boucher, Richard C.

doi:10.1074/jbc.m510520200

Cited by 26 publications

(16 citation statements)

References 62 publications

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“…The extent to which this model of protein interaction helps drive mucin packaging in secretory granules will depend on many local factors including calcium concentration, pH, and also the effects of the large glycosylated regions, which were not assessed in this study. These new results may also provide a mechanism to interpret previous proposals that MUC5B in secretory granules was organized around “nodes” formed by interaction between N-terminal regions (21) and may also explain the network appearance in TEM images of native mucin preparations (39) and pH-dependent protein-mediated linkages that were postulated to be important for intragranular condensation of mucin (40). …”

Section: Discussionsupporting

confidence: 67%

Assembly of the Respiratory Mucin MUC5B

Ridley

Kouvatsos

Raynal

et al. 2014

Journal of Biological Chemistry

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Background: Mucin polymer formation is a complex intracellular process.Results: MUC5B N-terminal D3-domains form reversible pH-sensitive calcium mediated cross-links between linear MUC5B polymer chains.Conclusion: Intracellular assembly of MUC5B generates disulfide-bonded polymers which form calcium mediated condensed networks in secretory granules.Significance: This identifies a new model for mucin assembly that may be common to other polymeric mucins.

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Section: Discussionsupporting

confidence: 67%

Assembly of the Respiratory Mucin MUC5B

Ridley

Kouvatsos

Raynal

et al. 2014

Journal of Biological Chemistry

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“…Studies of WT goblet cells showed that granule volume also tended to increase initially, but the change was apparently countered by coincident onset of exocytosis and granule loss. Our 2D area measurements of the granule cluster as an index of volume may be confounded by granule reorganization or changes in epithelial cell volume (35,53). However, direct observation of individual enlarging mucin granules within the Cftr-KO goblet cell is consistent with partial granule decondensation as a contributor to increased granular area.…”

Section: Discussionmentioning

confidence: 57%

Defective goblet cell exocytosis contributes to murine cystic fibrosis–associated intestinal disease

Liu¹,

Walker²,

Ootani³

et al. 2015

J. Clin. Invest.

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“…The cysteine rich domains form disulfide bonds to allow dimerization and multimerization which causes the gel-like mucus. For a more comprehensive review of gel-forming mucin structure see Perez-Vilar et al (Perez-Vilar et al, 2006). Gel-forming mucins are stored in secretory vesicles that fuse with the plasma membrane upon appropriate stimuli releasing mucins onto the mucosal surface (Dartt, 2004a; Evans et al, 2009; Perez-Vilar et al, 2006).…”

Section: Gel-forming Mucinsmentioning

confidence: 99%

“…O -glycosylation is a dynamic process and can be altered in disease. These mucins also undergo sulfation, sialylation, and proteolysis (Perez-Vilar et al, 2006). Gel-forming mucins also form disulfide bonds with one another to generate multimers (Perez-Vilar et al, 2006).…”

Section: Gel-forming Mucinsmentioning

confidence: 99%

“…These mucins also undergo sulfation, sialylation, and proteolysis (Perez-Vilar et al, 2006). Gel-forming mucins also form disulfide bonds with one another to generate multimers (Perez-Vilar et al, 2006). Hansson has demonstrated that in the stomach for MUC2 that the C termini of two molecules form disulfide bonds and the N-termini link into trimers (Hansson, 2012).…”

Section: Gel-forming Mucinsmentioning

confidence: 99%

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Tear film mucins: Front line defenders of the ocular surface; comparison with airway and gastrointestinal tract mucins

Hodges

Dartt

2013

Experimental Eye Research

155

103

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The ocular surface including the cornea and conjunctiva and its overlying tear film are the first tissues of the eye to interact with the external environment. The tear film is complex containing multiple layers secreted by different glands and tissues. Each layer contains specific molecules and proteins that not only maintain the health of the cells on the ocular surface by providing nourishment and removal of waste products but also protect these cells from environment. A major protective mechanism that the corneal and conjunctival cells have developed is secretion of the innermost layer of the tear film, the mucous layer. Both the cornea and conjunctiva express membrane spanning mucins, whereas the conjunctiva also produces soluble mucins. The mucins present in the tear film serve to maintain the hydration of the ocular surface and to provide lubrication and anti-adhesive properties between the cells of the ocular surface and conjunctiva during the blink. A third function is to contribute to the epithelial barrier to prevent pathogens from binding to the ocular surface. This review will focus on the different types of mucins produced by the corneal and conjunctival epithelia. Also included in this review will be a presentation of the structure of mucins, regulation of mucin production, role of mucins in ocular surface diseases, and the differences in mucin production by the ocular surface, airways and gastrointestinal tract.

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Mucin Granule Intraluminal Organization in Living Mucous/Goblet Cells

Cited by 26 publications

References 62 publications

Assembly of the Respiratory Mucin MUC5B

Assembly of the Respiratory Mucin MUC5B

Defective goblet cell exocytosis contributes to murine cystic fibrosis–associated intestinal disease

Tear film mucins: Front line defenders of the ocular surface; comparison with airway and gastrointestinal tract mucins

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