Mucin Family of Glycoproteins

Brown, Roger B.; Hollingsworth, Michael A.

doi:10.1016/b978-0-12-378630-2.00670-8

Cited by 9 publications

(11 citation statements)

References 9 publications

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“…The membrane-bound mucins characterized by the presence of the Muc domain (Pro/Thr/Ser) belong to an ever-increasing group of type I membrane-anchored proteins. Based on their structure and localization at the cell surface, they are thought to act in cell-cell and cell-matrix interactions, and in cell signaling pathways (Brown and Hollingsworth, 2013). Muc domains can be found in many bacteria genera and fungi (Valle Arevalo and Nobile, 2020) but are almost exclusively found in Firmicutes and specifically LAB isolated from the gastrointestinal tract.…”

Section: Adhesion Properties Of Kefir Microbiotamentioning

confidence: 99%

Invited review: Milk kefir microbiota—Direct and indirect antimicrobial effects

González‐Orozco

García‐Cano

Jiménez-Flores

et al. 2022

Journal of Dairy Science

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Kefir is a fermented dairy product with well recognized probiotic properties. Recently, consumer interest in fermented products with probiotic microorganisms has increased due to the accumulating evidence of the effects of kefir microorganisms on the modulation of gut microbiota and their antimicrobial activity. Although the health properties of kefir have been reviewed in other works, the present review addresses the antimicrobial effects of kefir microbiota and associated compounds. The antimicrobial activity of kefir microorganisms could derive from different mechanisms. The microorganisms' capacity to adhere to the intestinal epithelium, preventing the adhesion of pathogens, and their immunomodulation properties are among the mechanisms suggested. Bacteria and yeast isolated from kefir have been shown to have in vivo and in vitro antimicrobial activity against enteropathogenic bacteria and spoilage fungi. However, most reports have focused their approach on single-strain antimicrobial properties; evaluation of antimicrobial activity of cocultures of kefir microbiota and their potential mechanisms of action has been neglected. Kefir microbiota and associated compounds have shown promising antimicrobial effects; however, more research needs to be done to discern the mechanisms of action.

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Section: Adhesion Properties Of Kefir Microbiotamentioning

confidence: 99%

Invited review: Milk kefir microbiota—Direct and indirect antimicrobial effects

González‐Orozco

García‐Cano

Jiménez-Flores

et al. 2022

Journal of Dairy Science

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“…Structural analysis of the released oligosaccharides identified seven O -glycans from five different compositions (Table S3, Supporting Information). The small number of O -linked glycan structural types identified in this study is not surprising, as large O -glycan structural diversity tends to be present on secreted glycoproteins such as mucins , or mucin-like cell surface glycoproteins found on secretory epithelial cells …”

Section: Resultsmentioning

confidence: 99%

“…The small number of O-linked glycan structural types identified in this study is not surprising, as large O-glycan structural diversity tends to be present on secreted glycoproteins such as mucins 43,44 or mucin-like cell surface glycoproteins found on secretory epithelial cells. 45 As with the N-glycome, the average mass profiles of O-glycans over passaging were first confirmed to be qualitatively unchanged over cell culture passaging (Supporting Information Figure S5b). Qualitatively, the O-glycome did not differ between ADSCs, and adipocytes, with the exception of one isomeric structure with m/z [675.3] 1− (structure O1), which was not identified in ADSCs.…”

Section: Identification Of Structures Containing a Terminal Gal−gal E...mentioning

confidence: 99%

Bisecting GlcNAc Protein N-Glycosylation Is Characteristic of Human Adipogenesis

2020

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Human adipose tissue contains a major source of adipose-derived stem cells (ADSCs) that have the ability to differentiate into various cell types: in vitro, ADSCs can differentiate into mesenchymal lineages including adipocytes, while in vivo, ADSCs become mature adipocytes. Protein glycosylation has been shown to change in stem cell differentiation, and while ADSCs have been acknowledged for their therapeutic potential, little is known about protein glycosylation during human ADSC adipogenic differentiation. In the present study, the global membrane protein glycosylation of native adipocytes was compared to ADSCs from the same individuals as a model of in vivo adipogenesis. For in vitro adipogenesis, ADSCs were adipogenically differentiated in cell culture using an optimized, large-scale differentiation procedure. The membrane glycome of the differentiated ADSCs (dADSCs) was compared with mature adipocytes and the progenitor ADSCs. A total of 137 glycan structures were characterized across the three cell types using PGC-LC coupled with negative-ion electrospray ionization mass spectrometry (ESI-MS)/MS. Significantly higher levels of bisecting GlcNAc-type N-glycans were detected in mature adipocytes (32.1% of total glycans) and in in vitro dADSC progeny (1.9% of total glycans) compared to ADSCs. This was further correlated by the mRNA expression of the MGAT3 gene responsible for the enzymatic synthesis of this structural type. The bisecting GlcNAc structures were found on the majority of human native adipocyte membrane proteins, suggesting an important role in human adipocyte biology. Core fucosylation was also significantly increased during in vivo adipogenesis but did not correlate with an increase in Fut8 gene transcript. Unexpectedly, low abundance structures carrying rare β-linked Gal−Gal termini were also detected. Overall, the N-glycan profiles of the in vitro differentiated progeny did not reflect native adipocytes, and the results show that bisecting GlcNAc structures are a characteristic feature of human adipocyte membrane protein N-glycosylation. Raw MS files are available on GlycoPOST (ID: GPST000153 https://glycopost.glycosmos.org/).

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“…The pirarucu rectum has a high concentration of goblet cells that have the function of protecting the mucosa in the rectum and lubricating the faeces (Rodrigues & Cargnin‐Ferreira, 2017). The intestinal mucus in fish is composed of glycoproteins, mainly mucins (Brown & Hollingsworth, 2013), which play an important role in protecting the intestinal epithelium against pathogens (Salinas & Parra, 2015). The high concentration of goblet cells probably reflects a high production of mucus in this region and contribute to the formation of the transparent layer with bubbles that surrounds the pirarucu stool and makes it float.…”

Section: Discussionmentioning

confidence: 99%

Optimizing methodological aspects of stool collection by sedimentation in digestibility studies with pirarucu ( Arapaima gigas )

Ramos

Fracalossi

Freitas

et al. 2021

Aquaculture Research

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This study aimed at optimizing the sedimentation method for stool collection in digestibility tests in pirarucu. In the first trial, juvenile fish (435.26 ± 10.80 g) received the reference diet with chromium oxide to determine the gastrointestinal transit time (GITT). In the second, the fish were fed the reference and two test diets (corn and soybean meal) for 15 days. The apparent dry matter, protein and phosphorus digestibility coefficients (ADC) were determined to verify the time of acclimatization to the experimental diets. The stool pattern was visually analysed on day 5 and 10 of acclimatization. The third trial evaluated the maximum stool collection time for reduced nutrient leaching 30 min, 1, 6 and 12 h after evacuation as well as the stool collected directly from the rectum. GITT was 5.5 h. ADC varied during the initial acclimatization and stabilized between days 8 and 10, corroborating a consistent visual pattern on day 10 than on day 5. Nutrient ADC increased with increased stool collection time, except with a 30‐min interval, which presented ADC similar to rectum samples. Pirarucu stool must be collected from day 10 for a 30‐min interval about 30 min after the second daily meal.

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Mucin Family of Glycoproteins

Cited by 9 publications

References 9 publications

Invited review: Milk kefir microbiota—Direct and indirect antimicrobial effects

Invited review: Milk kefir microbiota—Direct and indirect antimicrobial effects

Bisecting GlcNAc Protein N-Glycosylation Is Characteristic of Human Adipogenesis

Optimizing methodological aspects of stool collection by sedimentation in digestibility studies with pirarucu ( Arapaima gigas )

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