Mucin adsorption on vaterite CaCO3 microcrystals for the prediction of mucoadhesive properties

Encapsulation of Low-Molecular-Weight Drugs into Polymer Multilayer Capsules Templated on Vaterite CaCO3 Crystals

“…Hence, it is assumed that the drug release rate is controlled with respect of the mucin content in the hybrids, of which the release profiles demonstrate, also presented in Figure 8B. This study holds great implications for the controlled release of low molecular weight substances via simple tailoring of the matrix content within the CaCO3 crystals prior to coating, and perhaps may be incorporated into mucin-based multilayer capsules, as previously reported [105].…”

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

“…Particularly, LbL technology presented several advantages for biomedicine: (i) deposition of homogeneous films with controlled thickness, (ii) high loading capacities and controlled release of biomolecules/drugs of various nature, and (iii) coating stability under physiological conditions. This made the LbL method one of the most rapidly growing strategies for generating thin film coatings of biomedical scaffolds [ 3 , 4 , 5 , 6 ], patterned surfaces [ 7 , 8 ], medical devices [ 9 , 10 ], implants [ 11 , 12 ], and a range of alternate bioapplications ( Figure 1 ); while multilayer capsules became promising nano- and micro-carriers for drug delivery applications [ 1 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ].…”

Inter-protein interactions govern protein loading into porous vaterite CaCO3 crystals

“…Interestingly, recently it has been shown that in contrast to these proteins, the adsorption of mucin is controlled by its retarded diffusion inside the pores of vaterite crystals. 42 This comparison indicates that the spreading dynamics of compact globular proteins is less affected by their interaction with the walls of the CaCO 3 pores when compared to the large and nonglobular mucin, which is known for its strong adhesiveness. Although accurate evaluation of the impact of the protein-wall interactions on the actual law of diffusion of these proteins is challengeable and has not been performed thus far (as has been shown for the diffusion of other molecules in crowded and non-inert matrices 72,73 ), it can be assumed that these specific interactions are not strong enough to be determinative for the overall adsorption kinetics.…”

“…27,28 In addition, CaCO 3 crystals can be used as sacrificial templates for the formulation of porous protein 29 and polymer beads, 30,31 and layer-by-layer assemblies to produce multilayer capsules. [32][33][34][35] All these findings indicate that CaCO 3 crystals are promising carriers for the encapsulation of protein-based therapeutics for oral, [36][37][38] intravenous, 17 intratumoral, 39 intranasal, 40 and mucosal drug delivery; 41,42 tissue engineering 43,44 and regeneration; 45,46 and diagnostics. 47,48 This diversity of administration routes enabled by CaCO 3 for protein delivery is especially important because in current medical practice, protein drugs are predominantly available in injectable formulations, but the demand for non-invasive drug delivery systems for therapeutic proteins is high.…”