MUC18, a member of the immunoglobulin superfamily, is expressed on bone marrow fibroblasts and a subset of hematological malignancies

Abstract

Despite the importance of bone marrow stromal cells in hemopoiesis, the profile of surface molecule expression is relatively poorly understood. Mice were immunized with cultured human bone marrow stromal cells in order to raise monoclonal antibodies to novel cell surface molecules, which might be involved in interactions with hemopoietic cells. Three antibodies, WM85, CC9 and EB4 were produced, and were found to identify a 100-110 kDa antigen on bone marrow fibroblasts.

Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells

“…[2][3][4][5] In addition, there has been only scattered data on the phenotype of the native BM MSCs because different markers have been used for MSC isolation in independent studies. [6][7][8][9][10][11][12][13][14][15][16][17] In this work we have studied the plasma membrane protein phenotype of cultured cells, screening for molecules not detected in hematopoietic cells. Hierarchical and principal components analyses of the transcripts coding for membrane proteins and distribution of the membrane antigens indicate lineagehomogeneity.…”

Differential CD146 Expression on Circulating Versus Tissue Endothelial Cells in Rectal Cancer Patients: Implications for Circulating Endothelial and Progenitor Cells As Biomarkers for Antiangiogenic Therapy

“…12,14,15 But other in vitro studies and more recent in vivo data questioned the specificity of CD146 expression for CECs. 14,[16][17][18] Yet, CD146 is extensively used to identify circulating cells of endothelial lineage and to distinguish them from leukocytes in preclinical and clinical studies. 3,12,13,19,20 Therefore, the identification of CECs and progenitor cell populations by CD146 must be rigorously validated in the blood of cancer patients.…”

Influence of MUC18/MCAM/CD146 expression on human melanoma growth and metastasis in SCID mice

“…MCAM belongs to the immunoglobulin superfamily and shows both structural and sequence similarity to gicerin/HEMCAM and BEN/SC1/DM-GRASP/ALCAM, cell adhesion molecules involved in the development of the nervous and hematopoietic systems (Vainio et al, 1996, Pourquie et al, 1992. In normal adult tissues, MCAM is primarily expressed by smooth muscle and vascular endothelium (Shih et al, 1994;Sers et al, 1993;Bardin et al, 1996) but has also been detected on a subpopulation of activated T lymphocytes (Pickl et al, 1997) and in the bone marrow (Filshie et al, 1998).…”