MUC13 promotes the development of colitis-associated colorectal tumors via β-catenin activity

Sheng, Yonghua; Wong, Kuan Yau; Seim, Inge; Wang, Ran; He, Yaowu; Wu, Alex; Patrick, Maya; Lourie, Rohan; Schreiber, Veronika; Giri, Rabina; Ng, Choa Ping; Popat, Amirali; Hooper, John D.; Kijanka, Gregor; Florin, Timothy H.; Begun, Jakob; Radford, Kristen J.; Hasnain, Sumaira Z.; McGuckin, Michael A.

doi:10.1038/s41388-019-0951-y

Cited by 30 publications

(28 citation statements)

References 52 publications

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“…For example, microbiota can feed on mucin glycans and convert them into SCFAs that supply colonocytes and other gut epithelial cells with energy ( Ouwerkerk et al, 2013 ). Interestingly, MUC13 is abundant in many adenocarcinomas ( Sheng et al, 2019 ), where it serves as a potential prognostic factor ( Filippou et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis

et al. 2021

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The use of metaproteomics for studying the human gut microbiota can shed light on the taxonomic profile and the functional role of the microbial community. Nevertheless, methods for extracting proteins from stool samples continue to evolve, in the pursuit of optimal protocols for moistening and dispersing the stool sample and for disrupting microbial cells, which are two critical steps for ensuring good protein recovery. Here, we evaluated different stool sample processing (SSP) and microbial cell disruption methods (CDMs). The combination of a longer disintegration period of the stool sample in a tube rotator with sonication increased the overall number of identified peptides and proteins. Proteobacteria, Bacteroidetes, Planctomycetes, and Euryarchaeota identification was favored by mechanical cell disruption with glass beads. In contrast, the relative abundance of Firmicutes, Actinobacteria, and Fusobacteria was improved when sonication was performed before bead beating. Tenericutes and Apicomplexa identification was enhanced by moistening the stool samples during processing and by disrupting cells with medium-sized glass beads combined with or without sonication. Human protein identifications were affected by sonication. To test the reproducibility of these gut metaproteomic analyses, we examined samples from six healthy individuals using a protocol that had shown a good taxonomic diversity and identification of proteins from Proteobacteria and humans. We also detected proteins involved in microbial functions relevant to the host and related mostly to specific taxa, such as B12 biosynthesis and short chain fatty acid (SCFA) production carried out mainly by members in the Prevotella genus and the Firmicutes phylum, respectively. The taxonomic and functional profiles obtained with the different protocols described in this work provides the researcher with valuable information when choosing the most adequate protocol for the study of certain pathologies under suspicion of being related to a specific taxon from the gut microbiota.

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Section: Resultsmentioning

confidence: 99%

Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis

et al. 2021

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“…Based on our findings, we conclude that the deletion of Muc4 may contribute to goblet cell dysfunction, resulting in alteration of mucin expression (Muc2), leading to dysbiosis. Along with MUC2, MUC13, MUC16, and MUC5AC have been implied in CRC progression, metastasis, and chemoresistance [ 42 – 44 ]. In the present study, we observed that Muc13 was upregulated in the absence of Muc4, suggesting its tumor-promoting role in CRC.…”

Section: Discussionmentioning

confidence: 99%

Depletion of transmembrane mucin 4 (Muc4) alters intestinal homeostasis in a genetically engineered mouse model of colorectal cancer

Pothuraju

Pai

Chaudhary

et al. 2022

Aging

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INTRODUCTIONColorectal cancer (CRC) occurs through a multi-step process (polyp-adenoma-carcinoma) in which several mutations are associated with disease progression [1,2].The most frequent mutations (40-70%) occur in the adenomatous polyposis coli (APC) gene, a component of Wnt/β-catenin signaling [2,3]. CRC is the third leading cause of cancer-related deaths in the United States of America and will account for approximately www.aging-us.com

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“…Previous studies revealed that altered apoptosis and proliferation in the early phases of tumor promotion in CAC models may affect the incidence and load of tumors. 26 , 27 Thus, attention was paid to these time points to investigate the molecular mechanism of promoting colorectal tumorigenesis by autophagy. To probe into whether autophagy deficiency renders IECs more susceptible to apoptosis in early tumor promotion, Atg7 f/f and Atg7 ΔIEC mice were treated with DSS for inducing acute colitis ( Figure 3 A).…”

Section: Resultsmentioning

confidence: 99%

Intestinal epithelial cell autophagy deficiency suppresses inflammation-associated colon tumorigenesis

Líu

Lou

Liu

et al. 2022

Molecular Therapy - Nucleic Acids

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MUC13 promotes the development of colitis-associated colorectal tumors via β-catenin activity

Cited by 30 publications

References 52 publications

Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis

Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis

Depletion of transmembrane mucin 4 (Muc4) alters intestinal homeostasis in a genetically engineered mouse model of colorectal cancer

Intestinal epithelial cell autophagy deficiency suppresses inflammation-associated colon tumorigenesis

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