MTT-assay and neutral red release (NRR)-assay: Relative role in the prediction of the irritancy potential of surfactants

Körting, Hans Christian; Schindler, Susanne; Hartinger, A.; Kerscher, Martina; Angerpointner, Thomas A.; Maibach, Howard I.

doi:10.1016/0024-3205(94)00746-2

Cited by 71 publications

(38 citation statements)

References 18 publications

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“…MTT assays were done as described by Korting et al (1994). n ϭ 3 independent cultures; 4 wells per condition per culture.…”

Section: Tunel and Mtt Assaysmentioning

confidence: 99%

Progranulin Deficiency Decreases Gross Neural Connectivity But Enhances Transmission at Individual Synapses

Tapia¹,

Milnerwood²,

Guo³

et al. 2011

J. Neurosci.

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Frontotemporal dementia (FTD) has been linked to mutations in the progranulin gene (GRN) that lead to progranulin (PGRN) haploinsufficiency. Thus far, our understanding of the effects of PGRN depletion in the brain has been derived from investigation of gross pathology, and more detailed analyses of cellular function have been lacking. We report that knocking down PGRN levels in rat primary hippocampal cultures reduces neural connectivity by decreasing neuronal arborization and length as well as synapse density. Despite this, the number of synaptic vesicles per synapse and the frequency of mEPSCs are increased in PGRN knockdown cells, suggesting an increase in the probability of release at remaining synapses. Interestingly, we demonstrate that the number of vesicles per synapse is also increased in postmortem brain sections from FTD patients with PGRN haploinsufficiency, relative to controls. Our observations show that PGRN knockdown severely alters neuronal connectivity in vitro and that the synaptic vesicle phenotype observed in culture is consistent with that observed in the hippocampus of FTD patients.

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“…MTT assays were done as described by Korting et al (1994). n ϭ 3 independent cultures; 4 wells per condition per culture.…”

Section: Tunel and Mtt Assaysmentioning

confidence: 99%

Progranulin Deficiency Decreases Gross Neural Connectivity But Enhances Transmission at Individual Synapses

Tapia¹,

Milnerwood²,

Guo³

et al. 2011

J. Neurosci.

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“…The effect of 2ME exposure on cell growth was spectrophotometrically determined by means of the MTT assay 22 .…”

Section: Determination Of Cell Growthmentioning

confidence: 99%

In vitro effects of 2‐methoxyestradiol on MCF‐12A and MCF‐7 cell growth, morphology and mitotic spindle formation

Zijl

Lottering

Steffens

et al. 2008

Cell Biochemistry & Function

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The influence of 2-methoxyestradiol (2ME) was investigated on cell growth, morphology and spindle formation in a tumorigenic (MCF-7) and non-tumorigenic (MCF-12A) epithelial breast cell line. Inhibition of cell growth was more pronounced in the MCF-7 cells compared to the MCF-12A cells following 2ME treatment. Dose-dependent studies (10 -5 -10 -9 M) revealed that 10 -6 M 2ME inhibited cell growth by 44% in MCF-12A cells and by 84% in MCF-7 cells (P-value < 0.05). 2ME-treated MCF-7 cells showed abnormal metaphase cells, membrane blebbing, apoptotic cells and disrupted spindle formation. These observations were either absent, or less prominent in MCF-12A cells. 2ME had no effect on the length of the cell cycle between S-phase and the time a mitotic peak was reached in either cell line but MCF-7 cells were blocked in mitosis with no statistically significant alterations in the phosphorylation status of Cdc25C.Nevertheless, Cdc2 activity was significantly increased in MCF-7 cells compared to MCF-12A cells (P-value < 0.05). The results indicate that 2ME disrupts mitotic spindle formation and enhances Cdc2 kinase activity, leading to persistence of the spindle checkpoint and thus prolonged metaphase arrest that may result in the induction of apoptosis. The tumorigenic MCF-7 cells were especially sensitive to 2ME treatment compared to the normal MCF-12A cells.Therefore, differential mechanism(s) of growth inhibition are evident between the normal and tumorigenic cells.

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“…The MTT colorimetric assay was used to assess viability based on mitochondrial enzyme function. In this assay, viable cells convert the MTT into a blue formazan product and the colorimetric reaction is measured by absorbance at 570 nm with a reference wavelength at 630 nm (22). MTT concentrate was diluted to 1 mg/ml in VEC-100 assay medium (both provided by MatTek).…”

Section: Test Agentsmentioning

confidence: 99%

Biocompatibility of Solid-Dosage Forms of Anti-Human Immunodeficiency Virus Type 1 Microbicides with the Human Cervicovaginal Mucosa Modeled Ex Vivo

Trifonova

Pasicznyk

Fichorova

2006

Antimicrob Agents Chemother

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MTT-assay and neutral red release (NRR)-assay: Relative role in the prediction of the irritancy potential of surfactants

Cited by 71 publications

References 18 publications

Progranulin Deficiency Decreases Gross Neural Connectivity But Enhances Transmission at Individual Synapses

Progranulin Deficiency Decreases Gross Neural Connectivity But Enhances Transmission at Individual Synapses

In vitro effects of 2‐methoxyestradiol on MCF‐12A and MCF‐7 cell growth, morphology and mitotic spindle formation

Biocompatibility of Solid-Dosage Forms of Anti-Human Immunodeficiency Virus Type 1 Microbicides with the Human Cervicovaginal Mucosa Modeled Ex Vivo

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