MTRR gene variant rs1801394 found in Malaysian patients with neural tube defects

Abstract

<jats:p>Neural tube defects (NTDs) are congenital anomalies resulting from the failure of neural tube closure during embryogenesis. The precise molecular mechanisms underlying this multifactorial disease is poorly understood, although single nucleotide polymorphisms in genes involved in the one-carbon metabolism cycle are believed to contribute towards NTD development. Among them is 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR). Protein function prediction algorithms (PolyPhen-2, PROVEAN, SIFT, SMART-Ensembl) were employed to evaluate its pathogenicity potential caused by the replacement of isoleucine with methionine. Seven NTD patients and 12 of their parents were recruited for this study. DNA samples were collected through blood or saliva whereby the extracted DNAs were then sent for whole exome sequencing (WES). Zygosity of the variant was confirmed from WES data of each subject and further validated through polymerase chain reaction (PCR) and Sanger sequencing. The results revealed that 57% of patients and 83% of parents carried rs1801394 mutation in their MTRR gene, based on either homozygous (G/G) or heterozygous (A/G) genotypes. Bioinformatics analysis of this missense mutation predicted that this change is damaging to MTRR protein function by 2 of the 3 predictor algorithms and that the change from isoleucine to methionine amino acid affects flavodoxin domain of the protein. This impacts enzyme activity within the one-carbon metabolism pathway, which is linked to the aetiology of NTDs. From population databases, this variant was considered common with a MAF &gt;0.3, however, it was not found in the Singapore Genome Variation Project (SGVP), whose population is a closer representation of the Malaysian subjects investigated here. Hence, we explored the prevalence of this variant in other studies and found that its association with NTDs differed across populations worldwide. Finally, we conclude that rs1801394 may be an NTD risk factor in the Malaysian population and should be further investigated as a potential prenatal screening tool.</jats:p>

Eph and Ephrin Variants in Malaysian Neural Tube Defect Families

“…Whilst, in exome datasets of proband SB2A and father of proband SB2A (SB2C), with both heterozygous for EPHA2 variant (rs147977279), 2 different variants in MTRR (rs162036, and rs10380) were also annotated as heterozygous in SB2A but not in SB2C (wildtype). Our previous study on the same cohort revealed that 57% of patients and 83% of parents carried rs1801394 mutation in their MTRR gene, based on either homozygous (G/G) or heterozygous (A/G) genotypes (Tan et al, 2020). In that study, we concluded that MTRR rs1801394 variant may be an NTD risk factor in the Malaysian population based on the prevalence of this variant in other studies and found that its association with NTDs differed across populations worldwide (Tan et al, 2020).…”

“…Our previous study on the same cohort revealed that 57% of patients and 83% of parents carried rs1801394 mutation in their MTRR gene, based on either homozygous (G/G) or heterozygous (A/G) genotypes (Tan et al, 2020). In that study, we concluded that MTRR rs1801394 variant may be an NTD risk factor in the Malaysian population based on the prevalence of this variant in other studies and found that its association with NTDs differed across populations worldwide (Tan et al, 2020). Hereto, the polymorphisms in a Eph and ephrin genes, single one-carbon metabolism gene, or planar cell polarity genes could play a limited role in overall NTD risk determination (Greene et al, 2009;Relton et al, 2004;Kibar et al, 2011;Kibar et al, 2007).…”

Eph and Ephrin Variants in Malaysian Neural Tube Defect Families

“…Moreover, in exome datasets of proband SB2A and the father of proband SB2A (SB2C), who are both heterozygous for EPHA2 variant (rs147977279), two different variants in MTRR (rs162036, and rs10380) were also annotated as heterozygous in SB2A but not in SB2C (wildtype). Our previous study on the same cohort revealed that 57% of patients and 83% of parents carried the rs1801394 variant in their MTRR gene, based on either homozygous (G/G) or heterozygous (A/G) genotypes [ 66 ]. In that study, we concluded that the MTRR rs1801394 variant may be an NTD risk factor in the Malaysian population based on the prevalence of this variant in other studies and that its association with NTDs differed across populations worldwide [ 66 ].…”

“…Our previous study on the same cohort revealed that 57% of patients and 83% of parents carried the rs1801394 variant in their MTRR gene, based on either homozygous (G/G) or heterozygous (A/G) genotypes [ 66 ]. In that study, we concluded that the MTRR rs1801394 variant may be an NTD risk factor in the Malaysian population based on the prevalence of this variant in other studies and that its association with NTDs differed across populations worldwide [ 66 ]. Hereto, the polymorphisms in Eph and ephrin genes, single one-carbon metabolism genes or planar cell polarity genes could play a limited role in overall NTD risk determination [ 10 , 67 , 68 , 69 ].…”