mTORC1 signalling mediates PI3K-dependent large lipid droplet accumulation in<i>Drosophila</i>ovarian nurse cells

Mensah, Lawrence B.; Goberdhan, Deborah C. I.; Wilson, Clive

doi:10.1242/bio.022210

Cited by 8 publications

(6 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also related to lipid metabolism in many cancers such as gastric cancer [ 57 ], hepatocellular carcinoma [ 58 ] and breast cancer [ 59 ]. Recent reports have showed that the activation of the PI3K signaling pathway is linked to lipid synthesis [ 43 ] and lipid droplet accumulation [ 60 ] in OC. In our study, we proved that FOXC2, STC1 and ITGB6 could activate the PI3K signaling pathway in OC cells and that this process may be related to lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer

Lin

Wang

et al. 2022

J Exp Clin Cancer Res

View full text Add to dashboard Cite

Background Stanniocalcin 1 (STC1) plays an integral role in ovarian cancer (OC). However, the functional role of STC1 in metastasis, lipid metabolism and cisplatin (DDP) chemoresistance in OC is not fully understood. Methods Single-cell sequencing and IHC analysis were performed to reveal STC1 expression profiles in patient tissues. Metastasis, lipid metabolism and DDP chemoresistance were subsequently assessed. Cell-based in vitro and in vivo assays were subsequently conducted to gain insight into the underlying mechanism of STC1 in OC. Results Single-cell sequencing assays and IHC analysis verified that STC1 expression was significantly enhanced in OC tissues compared with para-carcinoma tissues, and it was further up-regulated in peritoneal metastasis tissues compared with OC tissues. In vitro and in vivo experiments demonstrated that STC1 promoted metastasis, lipid metabolism and DDP chemoresistance in OC. Simultaneously, STC1 promoted lipid metabolism by up-regulating lipid-related genes such as UCP1, TOM20 and perilipin1. Mechanistically, STC1 directly bound to integrin β6 (ITGB6) to activate the PI3K signaling pathway. Moreover, STC1 was directly regulated by Forkhead box C2 (FOXC2) in OC. Notably, targeting STC1 and the FOXC2/ITGB6 signaling axis was related to DDP chemoresistance in vitro. Conclusions Overall, these findings revealed that STC1 promoted metastasis, lipid metabolism and DDP chemoresistance via the FOXC2/ITGB6 signaling axis in OC. Thus, STC1 may be used as a prognostic indicator in patients with metastatic OC. Meanwhile, STC1 could be a therapeutic target in OC patients, especially those who have developed chemoresistance to DDP.

show abstract

Section: Discussionmentioning

confidence: 99%

Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer

Lin

Wang

et al. 2022

J Exp Clin Cancer Res

View full text Add to dashboard Cite

show abstract

“…In Crassostrea gigas , PTEN is involved in insulin pathway in gonads and plays a critical role in reproduction [ 65 ]. In Drosophila , loss of PTEN is related to IIS/mTORC1 signalling, which is important for oogenesis [ 66 ]. In the present study, F3nRR showed significantly low expression levels of PTEN , which may have caused F3nRR normal fertility through regulation of oogenesis.…”

Section: Discussionmentioning

confidence: 99%

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

et al. 2021

View full text Add to dashboard Cite

Background Formation of triploid organism is useful in genetics and breeding. In this study, autotriploid Carassius auratus (3nRR, 3n = 150) was generated from Carassius auratus red var. (RCC, 2n = 100) (♀) and autotetraploid Carassius auratus (4nRR, 4n = 200) (♂). The female 3nRR produced haploid, diploid and triploid eggs, whereas the male 3nRR was infertile. The aim of the present study was to explore fertility of potential candidate genes of 3nRR. Results Gonadal transcriptome profiling of four groups (3 females RCC (FRCC), 3 males 4nRR (M4nRR), 3 males 3nRR (M3nRR) and 3 females 3nRR (F3nRR)) was performed using RNA-SEq. A total of 78.90 Gb of clean short reads and 24,262 differentially expressed transcripts (DETs), including 20,155 in F3nRR vs. FRCC and 4,107 in M3nRR vs. M4nRR were identified. A total of 106 enriched pathways were identified through KEGG enrichment analysis. Out of the enriched pathways, 44 and 62 signalling pathways were identified in F3nRR vs. FRCC and M3nRR vs. M4nRR, respectively. A total of 80 and 25 potential candidate genes for fertility-related in F3nRR and M3nRR were identified, respectively, through GO, KEGG analyses and the published literature. Moreover, protein-protein interaction (PPI) network construction of these fertility-associated genes were performed. Analysis of the PPI networks showed that 6 hub genes (MYC, SOX2, BMP4, GATA4, PTEN and BMP2) were involved in female fertility of F3nRR, and 2 hub genes (TP53 and FGF2) were involved in male sterility of M3nRR. Conclusions Establishment of autotriploid fish offers an ideal model to study reproductive traits of triploid fish. RNA-Seq data revealed 6 genes, namely, MYC, SOX2, BMP4, GATA4, PTEN and BMP2, involved in the female fertility of the F3nRR. Moreover, 2 genes, namely, TP53 and FGF2, were related to the male sterility of the M3nRR. These findings provide information on reproduction and breeding in triploid fish.

show abstract

“…Fundamentally, though, the inception and “normal” progression of the mutant condition or the diseased state can be closely followed, for example, the relationship between sleep and metabolism alterations via insulin signaling [ 219 – 221 ]. Unfortunately, to this day few studies have consciously addressed these chronic aspects of diabetes, although some do compare flies at different stages, like during oogenesis [ 222 ], or pupariation [ 223 ], or neurite remodeling [ 224 ], or adult stages [ 225 ].…”

Section: Invertebrate Insulin Signalingmentioning

confidence: 99%

Drosophila melanogasteras a Model for Diabetes Type 2 Progression

Álvarez-Rendón

Salceda

Riesgo-Escovar

2018

BioMed Research International

View full text Add to dashboard Cite

Drosophila melanogaster has been used as a very versatile and potent model in the past few years for studies in metabolism and metabolic disorders, including diabetes types 1 and 2. Drosophila insulin signaling, despite having seven insulin-like peptides with partially redundant functions, is very similar to the human insulin pathway and has served to study many different aspects of diabetes and the diabetic state. Yet, very few studies have addressed the chronic nature of diabetes, key for understanding the full-blown disease, which most studies normally explore. One of the advantages of having Drosophila mutant viable combinations at different levels of the insulin pathway, with significantly reduced insulin pathway signaling, is that the abnormal metabolic state can be studied from the onset of the life cycle and followed throughout. In this review, we look at the chronic nature of impaired insulin signaling. We also compare these results to the results gleaned from vertebrate model studies.

show abstract

mTORC1 signalling mediates PI3K-dependent large lipid droplet accumulation inDrosophilaovarian nurse cells

Cited by 8 publications

References 64 publications

Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer

Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

Drosophila melanogasteras a Model for Diabetes Type 2 Progression

Contact Info

Product

Resources

About