MtlR negatively regulates mannitol utilization by Vibrio cholerae

Byer, Tanner; Wang, Jessica; Zhang, Mark; Vather, Naomi; Blachman, Anna; Visser, Bryan J.; Liu, Jane M.

doi:10.1099/mic.0.000559

Cited by 12 publications

(11 citation statements)

References 37 publications

(36 reference statements)

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“…Mannitol is a polyol produced by marine algae and is the most abundant hexitol found in fungi, algae, and plants [ 25 , 26 ]. Mannitol metabolism has been intensively studied in marine bacteria, such as Vibrio cholerae and Zobellia galactanivorans [ 26 , 27 ]. Furthermore, mannitol is widely used in food, pharmaceutical, medical and chemical industries [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Whole-Genome-Based Survey for Polyphyletic Serovars of Salmonella enterica subsp. enterica Provides New Insights into Public Health Surveillance

Yin

Liu

et al. 2020

IJMS

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Serotyping has traditionally been considered the basis for surveillance of Salmonella, but it cannot distinguish distinct lineages sharing the same serovar that vary in host range, pathogenicity and epidemiology. However, polyphyletic serovars have not been extensively investigated. Public health microbiology is currently being transformed by whole-genome sequencing (WGS) data, which promote the lineage determination using a more powerful and accurate technique than serotyping. The focus in this study is to survey and analyze putative polyphyletic serovars. The multi-locus sequence typing (MLST) phylogenetic analysis identified four putative polyphyletic serovars, namely, Montevideo, Bareilly, Saintpaul, and Muenchen. Whole-genome-based phylogeny and population structure highlighted the polyphyletic nature of Bareilly and Saintpaul and the multi-lineage nature of Montevideo and Muenchen. The population of these serovars was defined by extensive genetic diversity, the open pan genome and the small core genome. Source niche metadata revealed putative existence of lineage-specific niche adaptation (host-preference and environmental-preference), exhibited by lineage-specific genomic contents associated with metabolism and transport. Meanwhile, differences in genetic profiles relating to virulence and antimicrobial resistance within each lineage may contribute to pathogenicity and epidemiology. The results also showed that recombination events occurring at the H1-antigen loci may be an important reason for polyphyly. The results presented here provide the genomic basis of simple, rapid, and accurate identification of phylogenetic lineages of these serovars, which could have important implications for public health.

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Section: Resultsmentioning

confidence: 99%

Whole-Genome-Based Survey for Polyphyletic Serovars of Salmonella enterica subsp. enterica Provides New Insights into Public Health Surveillance

Yin

Liu

et al. 2020

IJMS

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“…The MtlR repressor protein, which is encoded in the mtlADR operon itself, and MtlS appear to operate independently from each other (59,61). MtlA was shown to activate biofilm formation suggesting that mannitol serves as extracellular signal for V. cholerae to colonize beneficial habitats (62).…”

Section: Regulation Of Mannitol Uptake By a Cis-encoded Srna In Vibrimentioning

confidence: 99%

Carbohydrate Utilization in Bacteria: Making the Most Out of Sugars with the Help of Small Regulatory RNAs

Ðurica-Mitić

Göpel

Görke

2018

Regulating With RNA in Bacteria and Archaea

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Survival of bacteria in ever-changing habitats with fluctuating nutrient supplies requires rapid adaptation of their metabolic capabilities. To this end, carbohydrate metabolism is governed by complex regulatory networks including post-transcriptional mechanisms that involve small regulatory RNAs (sRNAs) and RNA-binding proteins. sRNAs limit the response to substrate availability, set the threshold or time required for induction and repression of carbohydrate utilization systems. Carbon catabolite repression (CCR) also involves sRNAs. In Enterobacteriaceae, sRNA Spot 42 cooperates with the transcriptional regulator cAMP-CRP to repress secondary carbohydrate utilization genes when a preferred sugar is consumed. In Pseudomonads, CCR operates entirely at the post-transcriptional level involving RNA-binding protein Hfq and decoy sRNA CrcZ. Moreover, sRNAs coordinate fluxes through central carbohydrate metabolic pathways with carbohydrate availability. In Gram-negative bacteria, the interplay between RNA-binding protein CsrA and its cognate sRNAs regulates glycolysis and gluconeogenesis in response to signals derived from metabolism. Spot 42 and cAMP-CRP jointly down-regulate tricarboxylic acid cycle activity when glycolytic carbon sources are ample. In addition, bacteria use sRNAs to reprogram carbohydrate metabolism in response to anaerobiosis and iron limitation. Finally, sRNAs also provide homeostasis of essential anabolic pathways as exemplified by the hexosamine pathway providing cell envelope precursors. In this review, we discuss the manifold roles of bacterial small RNAs for regulation of carbon source uptake and utilization, substrate prioritization and metabolism.

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“…cholerae which encodes three genes related to the transport and metabolism of mannitol: mtlA (encoding the mannitol-specific enzyme IIABC component of the phosphotransferase system [PTS]), mtlD (a mannitol-1-phosphate dehydrogenase), and mtlR (a transcriptional repressor of mtlA ) (Fig. 1A) (22–24). Mannitol is one of the most abundant and widely distributed natural sugar alcohols and the primary photosynthetic product of brown algae (25, 26).…”

Section: Introductionmentioning

confidence: 99%

“…The global regulator cAMP receptor protein (CRP) is a transcriptional activator of mtlA (29). Opposing the activity of CRP, MtlR acts as a transcriptional repressor of mtlA (23). Studies concerning the regulation of the mtl locus provide a model for maximal mtlA transcription that relies on two conditions: high cAMP-CRP activity and low MtlR activity (23, 29).…”

Section: Introductionmentioning

confidence: 99%

“…Opposing the activity of CRP, MtlR acts as a transcriptional repressor of mtlA (23). Studies concerning the regulation of the mtl locus provide a model for maximal mtlA transcription that relies on two conditions: high cAMP-CRP activity and low MtlR activity (23, 29). In glucose-containing medium, low cAMP levels preclude mtlA from being transcribed.…”

Section: Introductionmentioning

confidence: 99%

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Transcription of cis Antisense Small RNA MtlS in Vibrio cholerae Is Regulated by Transcription of Its Target Gene, mtlA

Zhang

Liu

2019

J Bacteriol

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Vibrio cholerae, the facultative pathogen responsible for cholera disease, continues to pose a global health burden. Its persistence can be attributed to a flexible genetic tool kit that allows for adaptation to different environments with distinct carbon sources, including the six-carbon sugar alcohol mannitol. V. cholerae takes up mannitol through the transporter protein MtlA, whose production is downregulated at the posttranscriptional level by MtlS, a cis antisense small RNA (sRNA) whose promoter lies within the mtlA open reading frame. Though it is known that mtlS expression is robust under growth conditions lacking mannitol, it has remained elusive as to what factors govern the steady-state levels of MtlS. Here, we show that manipulating mtlA transcription is sufficient to drive inverse changes in MtlS levels, likely through transcriptional interference. This work has uncovered a cis-acting sRNA whose expression pattern is predominantly controlled by transcription of the sRNA’s target gene. IMPORTANCE Vibrio cholerae is a bacterial pathogen that relies on genetic tools, such as regulatory RNAs, to adapt to changing extracellular conditions. While many studies have focused on how these regulatory RNAs function, fewer have focused on how they are themselves modulated. V. cholerae expresses the noncoding RNA MtlS, which can regulate mannitol transport and use, and here we demonstrate that MtlS levels are controlled by the level of transcription occurring in the antisense direction. Our findings provide a model of regulation describing how bacteria like V. cholerae can modulate the levels of an important regulatory RNA. Our work contributes to knowledge of how bacteria deploy regulatory RNAs as an adaptive mechanism to buffer against environmental flux.

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MtlR negatively regulates mannitol utilization by Vibrio cholerae

Cited by 12 publications

References 37 publications

Whole-Genome-Based Survey for Polyphyletic Serovars of Salmonella enterica subsp. enterica Provides New Insights into Public Health Surveillance

Whole-Genome-Based Survey for Polyphyletic Serovars of Salmonella enterica subsp. enterica Provides New Insights into Public Health Surveillance

Carbohydrate Utilization in Bacteria: Making the Most Out of Sugars with the Help of Small Regulatory RNAs

Transcription of cis Antisense Small RNA MtlS in Vibrio cholerae Is Regulated by Transcription of Its Target Gene, mtlA

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