Abstract:Background: Mitochondrial genome sequences have become critical to the study of biodiversity, though the cost of doing so with genome skimming or other methods has been difficult to reduce beyond ~$15–20 per sample making it costly to scale up. Here, we report on an approach to sequence hundreds to thousands of complete mitochondrial genomes in parallel using long-amplicon sequencing. We amplified the mitochondrial genome of 677 specimens in two partially overlapping amplicons and implemented an asymmetric PCR… Show more
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