MtCOPT2 is a Cu+ transporter specifically expressed in Medicago truncatula mycorrhizal roots

Senovilla, Marta; Abreu, Isidro; Escudero, Viviana; Cano, Custodia; Bago, Alberto; Imperial, Juan; González‐Guerrero, Manuel

doi:10.1007/s00572-020-00987-3

Cited by 17 publications

(7 citation statements)

References 63 publications

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“…Some studies have reported that mycorrhizal infection was sensitive to external P application, and thus, it affected the availability of other nutrients 15 , 16 . However, it was also reported that mycorrhizal fungi increased the uptake of Cu and contributed a 62% increase in Cu uptake irrespective of external P application 17 . Similarly, studies have highlighted the mycorrhizal fungi effect on Zn nutrition 7 , 18 .…”

Section: Introductionmentioning

confidence: 96%

Effect of arbuscular mycorrhizal fungi on the physiological functioning of maize under zinc-deficient soils

Saboor

Ali

Danish

et al. 2021

Sci Rep

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Zinc (Zn) deficiency can severely inhibit plant growth, yield, and enzymatic activities. Zn plays a vital role in various enzymatic activities in plants. Arbuscular mycorrhizal fungi (AMF) play a crucial role in improving the plant’s Zn nutrition and mitigating Zn stress effects on plants. The current study was conducted to compare the response of inoculated and non-inoculated maize (YH 1898) in the presence of different levels of zinc under greenhouse conditions under a Zn deficient condition. There were two mycorrhizal levels (i.e., M + with mycorrhizae, M- without mycorrhizae) and five Zn levels (i.e., 0, 1.5, 3, 6, and 12 mg kg-1), with three replicates following completely randomized design. At the vegetative stage (before tillering), biochemical, physiological, and agronomic attributes were measured. The results showed that maize plants previously inoculated with AMF had higher gaseous exchange traits, i.e., a higher stomatal conductance rate, favoring an increased photosynthetic rate. Improvement in antioxidant enzyme activity was also observed in inoculated compared to non-inoculated maize plants. Moreover, AMF inoculation also played a beneficial role in nutrients availability and its uptake by plants. Higher Zn12 (12 mg Zn kg-1 soil) treatment accumulated a higher Zn concentration in soil, root, and shoot in AMF-inoculated than in non-inoculated maize plants. These results are consistent with mycorrhizal symbiosis beneficial role for maize physiological functioning in Zn deficient soil conditions. Additionally, AMF inoculation mitigated the stress conditions and assisted nutrient uptake by maize.

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Section: Introductionmentioning

confidence: 96%

Effect of arbuscular mycorrhizal fungi on the physiological functioning of maize under zinc-deficient soils

Saboor

Ali

Danish

et al. 2021

Sci Rep

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“…Understanding metal homeostasis in nodules will also be relevant to our understanding of how metals are exchanged in other beneficial plant-microbe partnerships. For instance, the study of copper transport in M. truncatula nodules (Senovilla et al, 2018) led to the identification of a specific copper transporter of arbuscular mycorrhiza (Senovilla et al, 2020), highlighting the close evolutionary relationship between these two symbioses. More broadly, nodules have proven to be an excellent system to study plant metal homeostasis largely avoiding functional redundancy that might mask physiological roles.…”

Section: Discussionmentioning

confidence: 99%

Forging a symbiosis: transition metal delivery in symbiotic nitrogen fixation

et al. 2023

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SummarySymbiotic nitrogen fixation carried out by the interaction between legumes and rhizobia is the main source of nitrogen in natural ecosystems and in sustainable agriculture. For the symbiosis to be viable, nutrient exchange between the partners is essential. Transition metals are among the nutrients delivered to the nitrogen‐fixing bacteria within the legume root nodule cells. These elements are used as cofactors for many of the enzymes controlling nodule development and function, including nitrogenase, the only known enzyme able to convert N2 into NH3. In this review, we discuss the current knowledge on how iron, zinc, copper, and molybdenum reach the nodules, how they are delivered to nodule cells, and how they are transferred to nitrogen‐fixing bacteria within.

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“…Sand quartz was twice autoclaved (121 °C/2 h) over a 2-day interval. For the AM fungus inoculated treatment (AM), 50 ml (80 g) sterilized sand were mixed with 25 mg (a ratio of 0.5 g L −1 substrate) Osmocote Exact Mini 3–4 months (NPK 15:3.9:9.1 + 1.2 Mg + trace elements, ICL Specialty Fertilizers, UK) (Senovilla et al 2020 ), and 160 mg (a ratio of 3.2 g L −1 substrate) AM fungus inoculum (Mercy et al 2017 ) was added to each plastic pot (5 cm of height, 4 cm of width and with 3 holes in the bottom). For the negative control without inoculum (NM), the same amount of sand mixed with 25 mg Osmocote Exact Mini was filled into each plastic pot.…”

Section: Methodsmentioning

confidence: 99%

Rhizophagus irregularis improves Hg tolerance of Medicago truncatula by upregulating the Zn transporter genes ZIP2 and ZIP6

et al. 2023

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Mercury (Hg) pollution of soils is a critical environmental problem. To rehabilitate Hg contaminated soils, arbuscular mycorrhizal (AM) fungi-based phytoremediation may be supportive, yet the functional potential of AM fungi in response to Hg exposure is unclear. In a greenhouse experiment, we assessed the response of Medicago truncatula (Hg tolerance index (TI), Hg partitioning) to different Hg concentrations [0 (Hg0), 25 (Hg25), 50 (Hg50) µg g−1] in treatments with (AM) and without (NM) inoculation of Rhizophagus irregularis. Additionally, zinc (Zn) uptake and the expression of two Zn transporter genes (ZIP2, ZIP6) were examined because Zn is an essential element for plants and shares the same outer electronic configuration as Hg, implying potential competition for the same transporters. The results showed that AM plants had a higher TI than NM plants. Plant roots were identified as dominant Hg reservoirs. AM inoculation reduced the root Hg concentration under Hg50 compared to the NM treatment. There was an interaction between Hg treatment and AM inoculation on Hg stem concentration, i.e., at Hg25, AM inoculation decreased the Hg translocation from roots to stems, while Hg translocation was increased at Hg50 compared to the NM treatment. Zn acquisition was improved by R. irregularis. The negative relationship between Hg and Zn concentrations in the roots of AM and NM plants implied potential competition for the same transporters, although the expression of Zn transporters was upregulated by AM inoculation at all Hg levels. In conclusion, this baseline study demonstrated that R. irregularis may play an important role in Hg tolerance of M. truncatula, suggesting its potential for Hg-contaminated phytoremediation.

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MtCOPT2 is a Cu+ transporter specifically expressed in Medicago truncatula mycorrhizal roots

Cited by 17 publications

References 63 publications

Effect of arbuscular mycorrhizal fungi on the physiological functioning of maize under zinc-deficient soils

Effect of arbuscular mycorrhizal fungi on the physiological functioning of maize under zinc-deficient soils

Forging a symbiosis: transition metal delivery in symbiotic nitrogen fixation

Rhizophagus irregularis improves Hg tolerance of Medicago truncatula by upregulating the Zn transporter genes ZIP2 and ZIP6

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