MTA1 promotes the invasion and migration of oral squamous carcinoma by inducing epithelial–mesenchymal transition via the hedgehog signaling pathway

Song, Qingcui; Wang, Bao‐Zhong; Liu, Meirong; Ren, Zhongxi; Fu, Ying; Zhang, Pu; Yang, Mengxiang

doi:10.1016/j.yexcr.2019.05.031

Cited by 15 publications

(13 citation statements)

References 34 publications

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“…Metalloproteinase have been reported to stimulate tumor cell invasion and migration [ 11 ]. Furthermore, MTA1 was found to promote invasion by depressing the transcriptional expression of E-cadherin [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

“…MTA1 has been reported as a key component of the nuclear remodeling and deacetylation complex, which regulates metastasis-associated gene expression, including cell migration and invasion [ 10 ]. Moreover, MTA1 could mediate the migration and invasion of cancers by regulating the phosphorylation of various intracellular proteins involved in signaling pathways, such as AKT, hypoxia, and hedgehog signaling [ 7 , 8 , 11 ]. MTA1 has been reported to promote cancer cell invasion by depressing the E-cadherin expression [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

Lv¹,

Huang²,

Lei³

et al. 2020

BMC Urol

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Background The metastasis-associated gene 1 (MTA1) has been extensively reported as a crucial oncogene, and its abnormal expression has been associated with the progression of numerous cancers. However, the role of MTA1 in renal cell carcinoma (RCC) progression and metastasis remains unclear. Herein, we investigated the expression of MTA1 and its role in RCC. Methods 109 matched clear cell RCCs (ccRCCs) and corresponding normal tissue samples were analyzed via immunohistochemistry to test the expression of MTA1. Human A498 cell lines were transfected with pcDNA3.1-Flag (control) or Flag-MTA1 to overexpress MTA1 or with specific interfering RNA (si-MTA1) or specific interfering negative control to knockdown MTA1 expression. Transfected cells were used in wound healing and transwell invasion assay. Quantitative real time polymerase chain reaction was used to assess the effect of MTA1 on MMP2/MMP9 and E-cadherin gene expression. Western blot was used to qualify the phosphorylation of p65. Results Herein, we found a significantly increased expression of MTA1 in 109 ccRCCs, compared to the corresponding normal tissue. In addition, the overexpression of MTA1 in A498 cells facilitated cell migration and invasion, while the down-regulation of MTA1 expression using specific interfering RNA sequences could decrease cell migration and invasion. Furthermore, we showed that MTA1 is up-regulated in ccRCCs, which contributes to the migration and invasion of human kidney cancer cells by mediating the expression of MMP2 and MMP9 through the NF-κB signaling pathway. Similarly, we found that MTA1 could regulate E-cadherin expression in RCCs. Conclusions MTA1 is overexpressed in RCC and is involved in the progression of RCC through NF-κB.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

Lv¹,

Huang²,

Lei³

et al. 2020

BMC Urol

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“…MTA1 acts as an oncogene in various cancers, 6 and promotes the process of cancer progression and metastasis by inducing EMT in epithelial cancers, 35 hepatocellular cancer, 36 non-small-cell lung cancer, 37 and other tumors. 38 , 39 , 40 Our previous studies have demonstrated that MTA1 is overexpressed in endometrial cancer and facilitates endometrial cancer cell proliferation, migration, and invasion. 8 , 9 Since endometriosis has similar characteristics to cancers, we investigated the role of MTA1 in endometriosis in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have revealed that MTA1 contributes to metastasis by promoting EMT in various cancers. 35 , 36 , 37 , 38 , 39 , 40 However, whether MTA1 promotes EMT in endometriosis remains unclear.…”

Section: Introductionmentioning

confidence: 99%

MTA1, a Target of Resveratrol, Promotes Epithelial-Mesenchymal Transition of Endometriosis via ZEB2

Kong

Zhou

et al. 2020

Molecular Therapy - Methods & Clinical Development

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Endometriosis is a benign disease that shares some malignant features. Epithelial-mesenchymal transition (EMT) is involved in the pathogenesis of endometriosis. Metastasis-associated protein 1 (MTA1) plays an important role in various cancers by promoting EMT, yet there are no studies on its function in endometriosis. In the present study, we found that MTA1 was highly expressed in the ectopic endometrium of endometriosis patients and that the expression of MTA1 was related to the revised American Fertility Society stage. MTA1 facilitated endometrial stroma cell proliferation, migration, and invasion by inducing EMT, and the promotion function and MTA1 expression were suppressed by resveratrol, a natural polyphenol. Moreover, we revealed that MTA1 induced EMT through interaction with ZEB2. The findings in a mouse endometriosis model further showed that MTA1 and ZEB2 were upregulated in ectopic tissues and that resveratrol inhibited the growth of ectopic lesions and expression of MTA1 and ZEB2. Taken together, we demonstrate that MTA1 is a protein that promotes EMT via interacting with ZEB2 in the pathogenesis of endometriosis, and may be a target of resveratrol.

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“…CSCs are also involved with the chemo‐ and radioresistance of tumors, and with their metastasis 6 . OSCC cells usually spread by a lymphatic route after invasion into the surrounding tissues which is typically associated with an epithelial‐mesenchymal transition program (EMT) 7 . However, to generate tumor metastasis, EMT cells need to return to the epithelial phenotype 8 .…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo characterization of cancer stem cell subpopulations in oral squamous cell carcinoma

Amôr

Buzo

Ortiz

et al. 2020

J Oral Pathology Medicine

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Background Despite advances in cancer diagnosis and therapeutics, the overall 5‐year survival rate of oral squamous cell carcinoma (OSCC) remains low. Tumor formation, progression, recurrence, and chemo‐resistance are associated with the presence of cancer stem cells (CSC) that show phenotypic heterogeneity, but how they influence tumor behavior remains poorly understood. We aimed to describe how two CSC phenotypes from an OSCC cell line, CD44HighESAHigh (Epi‐CSC) and CD44HighESALow (EMT‐CSC), behave in vitro and in vivo. Methods In vitro behavior of FACS‐sorted Epi‐CSC and EMT‐CSC from OSCC cells was characterized by their ability to form colonies, migrate, proliferate, and to invade a solid matrix. In vivo experiments were conducted in immunodeficient (NOD/SCID) mice by orthotopic xenografting of FACS‐sorted OSCC subpopulations. Results In vitro, the Epi‐CSC phenotype was more proliferative and generated more holoclones than the EMT phenotype. On the other hand, EMT‐CSC migrate and invaded more than Epi‐CSC cells in 3D culture, suggesting the CSC phenotype affects tumor cell behavior. When inoculated orthotopically into the tongues of immunodeficient mice, both subpopulations generated OSCC, but EMT‐CSC formed fewer and smaller tumors. Conclusions Our results suggest that while cells in the Epi‐CSC form the subpopulation that enables tumor growth, the EMT‐CSC are related to migration and invasion. Clinically, this may reflect the importance of Epi‐CSC for tumorigenesis and of the EMT‐CSC for metastasis and highlights that variation in the proportion of CSC phenotypes from patient to patient may be relevant to the design of individual treatment protocols.

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MTA1 promotes the invasion and migration of oral squamous carcinoma by inducing epithelial–mesenchymal transition via the hedgehog signaling pathway

Cited by 15 publications

References 34 publications

Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

MTA1, a Target of Resveratrol, Promotes Epithelial-Mesenchymal Transition of Endometriosis via ZEB2

In vitro and in vivo characterization of cancer stem cell subpopulations in oral squamous cell carcinoma

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