MT2 Melatonin Receptor Immunoreactivity in Neurons is Very High in the Aged Hippocampal Formation in Gerbils

Lee, Choong Hyun; Choi, Jung Hoon; Yoo, Ki‐Yeon; Park, Ok Kyu; Hwang, In Koo; You, Sung Hyun; Lee, Boo-Yong; Kang, Il‐Jun; Won, Moo‐Ho

doi:10.1007/s10571-009-9447-6

Cited by 7 publications

(7 citation statements)

References 38 publications

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“…Although the melatonin receptor has been observed in the hippocampus [23,24,27], NAS has also been detected in this region [5]. There are reports on the connections between antidepressants, such as fluoxetine, and NAS in synaptic plasticity and neurogenesis.…”

Section: Discussionmentioning

confidence: 99%

N-Acetylserotonin Increases Cell Proliferation and Differentiating Neuroblasts with Tertiary Dendrites through Upregulation of Brain-Derived Neurotrophic Factor in the Mouse Dentate Gyrus

et al. 2011

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ABSTRACT. In this study, we investigated the effects of N-acetylserotonin (NAS) on cell proliferation and neuroblast differentiation in the mouse dentate gyrus using anti-Ki67 and anti-doublecortin (DCX) antibodies. Ki67 is expressed in the nucleus or on the surface of chromosomes during all of the active phases of the cell cycle, and DCX is expressed in neuronal precursor cells as well as in immature neurons. At 17 weeks of age, 20 mg/kg of NAS or the same volume of vehicle was intraperitoneally administered once a day for 3 weeks. The animals were sacrificed 2 hr after the last vehicle or NAS treatment. NAS treatment significantly increased the number of Ki67-positive nuclei and DCX-immunoreactive neuroblasts with well-developed dendrites (tertiary dendrites) compared to the vehicle-treated group. However, the number of DCX-immunoreactive neuroblasts without tertiary dendrites was not changed. The administration of NAS also significantly increased the protein levels of brain-derived neurotrophic factor (BDNF) in the dentate gyrus. This result suggests that NAS significantly promotes cell proliferation and the number of differentiating neuroblasts with tertiary dendrites through an increase in BDNF levels in the mouse dentate gyrus.KEY WORDS: brain-derived neurotrophic factor, cell proliferation, dentate gyrus, N-acetylserotonin, neuroblast differentiation.

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Section: Discussionmentioning

confidence: 99%

N-Acetylserotonin Increases Cell Proliferation and Differentiating Neuroblasts with Tertiary Dendrites through Upregulation of Brain-Derived Neurotrophic Factor in the Mouse Dentate Gyrus

et al. 2011

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“…The brains were removed and postfixed with the same solution for 6 h. The brain tissues were sectioned by the method of our previous study (Lee et al 2010).…”

Section: Methodsmentioning

confidence: 99%

“…To examine age-related changes in GLP-1R immunoreactivity in the gerbil hippocampus, immunohistochemical staining was performed according to our method (Lee et al 2010). Rabbit anti-GLP-1R (1:50, Santa Cruz Biotechnology, Santa Cruz, CA) was used as primary antibody.…”

Section: Methodsmentioning

confidence: 99%

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Decreased Glucagon-Like Peptide-1 Receptor Immunoreactivity in the Dentate Granule Cell Layer from Adult in the Gerbil Hippocampus

et al. 2010

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In this study, we investigated age-related changes in glucagon-like peptide-1 receptor (GLP-1R) immunoreactivity and its protein levels in the gerbil hippocampus during normal aging. In the postnatal month 3 (PM 3) group, GLP-1R immunoreaction was well observed in neurons, especially pyramidal and non-pyramidal cells in the hippocampus proper, and granule and polymorphic cells in the dentate gyrus. In the hippocampus proper, GLP-1R immunoreactivity in neurons was maintained until PM 24. In the dentate gyrus, however, GLP-1R immunoreactivity in granule cells, not polymorphic cells, was hardly detected from PM 6. Western blot analysis also showed that age-dependent change patterns in GLP-1R protein levels in the gerbil hippocampus were similar to the immunohistochemical changes. These results indicate that GLP-1R immunoreactivity was markedly decreased in dentate granule cells from PM 6, showing that GLP-1R immunoreactivity and its protein levels were decreased in the adult and aged gerbil hippocampus.

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“…In the hippocampus, melatonin regulates both excitatory and inhibitory functions of neurons through MT2 receptors [20,21]. The altered levels of melatonin receptors have been reported in aging and neurological disorders [22][23][24][25][26]. Specific role of MT1/MT2 has been suggested in Alzheimer's disease, Parkinson's disease, Huntington's disease, and amyotrophic lateral sclerosis [23,[27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Promising Role of Melatonin as Neuroprotectant in Neurodegenerative Pathology

et al. 2014

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Melatonin treatment showed a potent neuroprotective action in experimental models and in clinical studies. However, the entire disease prevention is not observed with melatonin treatment. Therefore, findings have suggested its future use in combination therapies for neurological diseases. Several studies have showed its free radical scavenging, antioxidant property, antiapoptotic activity, and its action towards enhanced mitochondrial function. It has direct and indirect effects on mitochondrial functions. Neurodegenerative disease pathology includes the impaired mitochondrial functions and apoptotic death of neurons due to energy crisis which could be prevented with antiapoptotic activity of melatonin. However, for the therapeutic use of melatonin, researchers also need to pay attention towards the various intermediary events taking place in apoptotic death of neurons during disease pathology. Age-related neurological diseases include the decreased level of melatonin in neuronal death. Therefore, it is worthwhile to discuss about the different functions of melatonin in aspect of its antioxidative property, its role in the enhancement of mitochondrial function, and its antiapoptotic attributes. This review summarizes the reports to date showing the potent role of melatonin in experimental models and clinical trials and discussing the employment of melatonin as future potent neuroprotective agent.

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MT2 Melatonin Receptor Immunoreactivity in Neurons is Very High in the Aged Hippocampal Formation in Gerbils

Cited by 7 publications

References 38 publications

N-Acetylserotonin Increases Cell Proliferation and Differentiating Neuroblasts with Tertiary Dendrites through Upregulation of Brain-Derived Neurotrophic Factor in the Mouse Dentate Gyrus

N-Acetylserotonin Increases Cell Proliferation and Differentiating Neuroblasts with Tertiary Dendrites through Upregulation of Brain-Derived Neurotrophic Factor in the Mouse Dentate Gyrus

Decreased Glucagon-Like Peptide-1 Receptor Immunoreactivity in the Dentate Granule Cell Layer from Adult in the Gerbil Hippocampus

Promising Role of Melatonin as Neuroprotectant in Neurodegenerative Pathology

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