MT1 receptor expression and AA-NAT activity in lymphatic tissue following melatonin administration in male golden hamster

Vishwas, Dipanshu Kumar; Haldar, Chandana

doi:10.1016/j.intimp.2014.06.038

Cited by 13 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The release of proinflammatory cytokines, such as IL‐1β (in earlier studies, IL‐1), IL‐2, IL‐6, IL‐12, TNFα, and IFNγ in response to melatonin has been repeatedly observed in monocytes, monocyte‐derived cells and T‐helper cells type 1, of IL‐17 in non‐preactivated Th17 cells, and of IL‐1β, TNFα and IFNγ in splenocytes, too . Although most studies clearly point into this direction, deviating findings have also been published.…”

Section: Proinflammatory Actions As Revealed By Studies On Immune Cellsmentioning

confidence: 96%

Melatonin and inflammation—Story of a double‐edged blade

Hardeland

2018

Journal of Pineal Research

341

350

View full text Add to dashboard Cite

Melatonin is an immune modulator that displays both pro‐ and anti‐inflammatory properties. Proinflammatory actions, which are well documented by many studies in isolated cells or leukocyte‐derived cell lines, can be assumed to enhance the resistance against pathogens. However, they can be detrimental in autoimmune diseases. Anti‐inflammatory actions are of particular medicinal interest, because they are observed in high‐grade inflammation such as sepsis, ischemia/reperfusion, and brain injury, and also in low‐grade inflammation during aging and in neurodegenerative diseases. The mechanisms contributing to anti‐inflammatory effects are manifold and comprise various pathways of secondary signaling. These include numerous antioxidant effects, downregulation of inducible and inhibition of neuronal NO synthases, downregulation of cyclooxygenase‐2, inhibition of high‐mobility group box‐1 signaling and toll‐like receptor‐4 activation, prevention of inflammasome NLRP3 activation, inhibition of NF‐κB activation and upregulation of nuclear factor erythroid 2‐related factor 2 (Nrf2). These effects are also reflected by downregulation of proinflammatory and upregulation of anti‐inflammatory cytokines. Proinflammatory actions of amyloid‐β peptides are reduced by enhancing α‐secretase and inhibition of β‐ and γ‐secretases. A particular role in melatonin's actions seems to be associated with the upregulation of sirtuin‐1 (SIRT1), which shares various effects known from melatonin and additionally interferes with the signaling by the mechanistic target of rapamycin (mTOR) and Notch, and reduces the expression of the proinflammatory lncRNA‐CCL2. The conclusion on a partial mediation by SIRT1 is supported by repeatedly observed inhibitions of melatonin effects by sirtuin inhibitors or knockdown.

show abstract

Section: Proinflammatory Actions As Revealed By Studies On Immune Cellsmentioning

confidence: 96%

Melatonin and inflammation—Story of a double‐edged blade

Hardeland

2018

Journal of Pineal Research

341

350

View full text Add to dashboard Cite

show abstract

“…The majority of immune cells and organs express melatonin receptors. Melatonin increases MT1 activity in the thymus and the spleen and enhances DTH response in Syrian hamsters 21 . We therefore evaluated MT1 as a potential mediator of decreased adaptive immune response in offspring.…”

Section: Resultsmentioning

confidence: 93%

“…Exposure to light at night alters endocrine function 13 , and elimination of nightly melatonin secretion is the most pronounced endocrine effect of light at night. Melatonin is a potent immunomodulator and enhances immune responses by stimulating bone marrow proliferation, antigen presentation, and release of certain cytokines, namely IL-2 and IFN-γ 18 19 20 . The majority of immune cells and organs express melatonin receptors.…”

Section: Resultsmentioning

confidence: 99%

Parental Exposure to Dim Light at Night Prior to Mating Alters Offspring Adaptive Immunity

Cissé

Russart

Nelson

2017

Sci Rep

View full text Add to dashboard Cite

Exposure to dim light at night (dLAN) disrupts natural light/dark cycles and impairs endogenous circadian rhythms necessary to maintain optimal biological function, including the endocrine and immune systems. We have previously demonstrated that white dLAN compromises innate and cell mediated immune responses in adult Siberian hamsters (Phodopus sungorus). We hypothesized that dLAN has transgenerational influences on immune function. Adult male and female Siberian hamsters were exposed to either dark nights (DARK) or dLAN (~5 lux) for 9 weeks, then paired in full factorial design, mated, and thereafter housed under dark nights. Offspring were gestated and reared in dark nights, then tested as adults for cell-mediated and humoral immunity. Maternal exposure to dLAN dampened delayed type hypersensitivity (DTH) responses in male offspring. Maternal and paternal exposure to dLAN reduced DTH responses in female offspring. IgG antibodies to a novel antigen were elevated in offspring of dams exposed to dLAN. Paternal exposure to dLAN decreased splenic endocrine receptor expression and global methylation in a parental sex-specific manner. Together, these data suggest that exposure to dLAN has transgenerational effects on endocrine-immune function that may be mediated by global alterations in the epigenetic landscape of immune tissues.

show abstract

“…A logical but not less interesting finding from our study was the highly statistically significant association between the expression of the melatonin membrane receptors and AANAT; at lower enzymes levels, there were also lower receptors levels. These receptors are largely responsible for mediating the downstream effects of melatonin [38], and AANAT is the major enzyme in melatonin synthesis [39]. Thus, an increase in local synthesis of melatonin via AANAT during the early age coincides with an increase in the action of melatonin via MT 1 and MT 2 receptors.…”

Section: Discussionmentioning

confidence: 99%

Temporal expression patterns of the melatoninergic system in the human thymus of children

Cruz‐Chamorro

Álvarez-Sánchez

Escalante-Andicoechea

et al. 2019

Molecular Metabolism

View full text Add to dashboard Cite

ObjectivesTo obtain greater knowledge of the extra-pineal sources of melatonin during development, the amount of indolamine and the expression levels of the last two enzymes involved in its biosynthesis, Arylalkylamine N-acetyltransferase (AANAT) and acetylserotonin O-methyltransferase (ASMT), were analyzed in the human thymus from children from three different age groups (from days to years). The melatonin membrane and nuclear receptor expression levels also were studied.MethodsQuantitative reverse transcriptase PCR and western blot were performed to investigate the receptor and enzyme expression levels. The results were examined and correlated with the ages of the thymuses.ResultsWe found high levels of indolamine in the thymuses of newborns (younger than 1 month), which decreased during development; thymuses from the months (from 2 to 11 months) and years (from 1 to 12 years) groups showed lower levels. A similar decline was also observed in the mRNA of the AANAT enzyme and the expression levels of melatonin receptors. However, ASMT expression was exactly the opposite, with low levels in the newborn group and higher levels in the years group. Our results show that the thymic synthesis of melatonin occurs very early in childhood. Additionally, this is the first report that is focused on melatonin receptors expression in the human thymus.ConclusionConsidering the limited melatonin synthesis performed by the newborn pineal gland, we suggest that the high levels of melatonin found in human thymus in this experimental group arise from synthesis in the tissue itself, which could be contributing to the immune efficiency at the thymic level.

show abstract

MT1 receptor expression and AA-NAT activity in lymphatic tissue following melatonin administration in male golden hamster

Cited by 13 publications

References 36 publications

Melatonin and inflammation—Story of a double‐edged blade

Melatonin and inflammation—Story of a double‐edged blade

Parental Exposure to Dim Light at Night Prior to Mating Alters Offspring Adaptive Immunity

Temporal expression patterns of the melatoninergic system in the human thymus of children

Contact Info

Product

Resources

About