MsPG1 alleviated aluminum-induced inhibition of root growth by decreasing aluminum accumulation and increasing porosity and extensibility of cell walls in alfalfa (Medicago sativa)

Li, Jiaojiao; Su, Liantai; Lv, Aimin; Li, Yanbang; Zhou, Peng; An, Yuan

doi:10.1016/j.envexpbot.2020.104045

Cited by 20 publications

(7 citation statements)

References 54 publications

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“…Then the plasmids MsDHN1 pro ::GUS, MsABF2 pro ::GUS and 35S::GUS were introduced into tobacco leaves or hairy roots of alfalfa, as described above. The injected leaves or hairy roots were stained with GUS staining solution (Li et al., 2020). Transverse and vertical longitude resin chips of hairy roots expressing MsDHN1 pro ::GUS were performed according to Wang’s description (Wang et al., 2016).…”

Section: Methodsmentioning

confidence: 99%

Dehydrin MsDHN1 improves aluminum tolerance of alfalfa (Medicago sativaL.) by affecting oxalate exudation from root tips

Wen

Fan

et al. 2021

The Plant Journal

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SUMMARY A SK3‐type dehydrin MsDHN1 was cloned from alfalfa (Medicago sativa L.). Its function and gene regulatory pathways were studied via overexpression and suppression of MsDHN1 in alfalfa seedlings or hairy roots. The results showed that MsDHN1 is a typical intrinsically disordered protein that exists in the form of monomers and homodimers in alfalfa. The plant growth rates increased as a result of MsDHN1 overexpression (MsDHN1‐OE) and decreased upon MsDHN1 suppression (MsDHN1‐RNAi) in seedlings or hairy roots of alfalfa compared with the wild‐type or the vector line under Al stress. MsDHN1 interacting with aquaporin (AQP) MsPIP2;1 and MsTIP1;1 positively affected oxalate secretion from root tips and Al accumulation in root tips. MsABF2 was proven to be an upstream transcription factor of MsDHN1 and activated MsDHN1 expression by binding to the ABRE element of the MsDHN1 promoter. The transcriptional regulation of MsABF2 on MsDHN1 was dependent on the abscisic acid signaling pathway. These results indicate that MsDHN1 can increase alfalfa tolerance to Al stress via increasing oxalate secretion from root tips, which may involve in the interaction of MsDHN1 with two AQP.

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Section: Methodsmentioning

confidence: 99%

Dehydrin MsDHN1 improves aluminum tolerance of alfalfa (Medicago sativaL.) by affecting oxalate exudation from root tips

Wen

Fan

et al. 2021

The Plant Journal

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“…Class III peroxidase uses H 2 O 2 as a substrate to produce OH − radicals that enhance cell wall plastic extensibility and elongation by nonenzymatic cleavage of cell wall polysaccharides [31][32][33]. Because root development is directly related to cell wall loosening and elongation [34], the upregulated expression of this gene by LN in the A-NIL (Table S14) may participate in LN-promoted root growth by affecting these processes. Since the most enriched process of the DEGs between the pairwise NILs is phenylpropanoid biosynthesis, the relation of phenylpropanoid and root traits is worthy of attention.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Reveals the Contribution of QMrl-7B to Wheat Root Growth and Development

Zhang

et al. 2020

Preprint

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Backgound: Roots are the major organs for water and nutrient acquisition and substantially affect plant growth, development and reproduction. Improvements to root system architecture are highly important for increasing yield potential of bread wheat. QMrl-7B, a major stable quantitative trait locus (QTL) that controls maximum root length (MRL), strongly contributes to an improved root system in wheat. Results: To further analyse the biological functions of QMrl-7B in root development, two types of Triticum aestivum near isogenic lines (NILs), one with superior QMrl-7B alleles from cultivar Kenong 9204 (KN9204) and another with inferior QMrl-7B alleles from cultivar Jing 411 (J411), were subjected to transcriptomic analysis. Among all the mapped genes analysed, 4871 genes were identified as being differentially expressed between the pairwise NILs under different nitrogen (N) conditions, with 3543 genes expressed under normal-nitrogen (NN) condition and 2689 genes expressed under low-nitrogen (LN) condition. These genes encode proteins that include mainly NO3- transporters, phytohormone signalling components and transcription factors (TFs), indicating the presence of a complex regulatory network involved in root determination. In addition, among the 13524 LN-induced differentially expressed genes (DEGs) detected in this assay, 4308 were specifically expressed in the A-NIL which brings superior alleles, and 2463 were expressed specifically in the B-NIL which brings inferior alleles. These DEGs reflect different responses of the two types of NILs to varying N supplies, which likely involve LN-induced root growth. Conclusions: These results explain the better-developed root system and increased root vitality provided by the superior alleles of QMrl-7B and provide a deeper understanding of the genetic underpinnings of root traits, pointing to a valuable locus suitable for future breeding efforts for sustainable agriculture.

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“…Chandran et al (2008) stained the root tips of Medicago truncatula seedlings grown at 10-mm Al concentration with hematoxylin and showed that the root tips treated with Al for the longest time stained the darkest, indicating that they were the most severely damaged. Li et al (2020) found that Al stress significantly enhanced the expression of MsPG1 in the plasma membrane of Medicago sativa root apical epidermal cells, reduced the accumulation of Al in the cell wall, and improved the Al tolerance of M. sativa. However, some plants grown for a long time in acidic soils can chelate Al 3+ in the vicinity of the root zone with organic acids such as malic, citric, and oxalic acids secreted by the root system, forming Al-organic acid complexes, and thus reducing the transport of Al to the interior of the root cell and enhancing Al tolerance (Chauhan et al, 2021).…”

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confidence: 88%

Genome-wide analysis and identification of microRNAs in Medicago truncatula under aluminum stress

Yang²,

Tian³

et al. 2023

Front. Plant Sci.

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Numerous studies have shown that plant microRNAs (miRNAs) play key roles in plant growth and development, as well as in response to biotic and abiotic stresses; however, the role of miRNA in legumes under aluminum (Al) stress have rarely been reported. Therefore, here, we aimed to investigate the role of miRNAs in and their mechanism of Al tolerance in legumes. To this end, we sequenced a 12-strand-specific library of Medicago truncatula under Al stress. A total of 195.80 M clean reads were obtained, and 876 miRNAs were identified, of which, 673 were known miRNAs and 203 were unknown. A total of 55 miRNAs and their corresponding 2,502 target genes were differentially expressed at various time points during Al stress. Further analysis revealed that mtr-miR156g-3p was the only miRNA that was significantly upregulated at all time points under Al stress and could directly regulate the expression of genes associated with root cell growth. Three miRNAs, novel_miR_135, novel_miR_182, and novel_miR_36, simultaneously regulated the expression of four Al-tolerant transcription factors, GRAS, MYB, WRKY, and bHLH, at an early stage of Al stress, indicating a response to Al stress. In addition, legume-specific miR2119 and miR5213 were involved in the tolerance mechanism to Al stress by regulating F-box proteins that have protective effects against stress. Our results contribute to an improved understanding of the role of miRNAs in Al stress in legumes and provide a basis for studying the molecular mechanisms of Al stress regulation.

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MsPG1 alleviated aluminum-induced inhibition of root growth by decreasing aluminum accumulation and increasing porosity and extensibility of cell walls in alfalfa (Medicago sativa)

Cited by 20 publications

References 54 publications

Dehydrin MsDHN1 improves aluminum tolerance of alfalfa (Medicago sativaL.) by affecting oxalate exudation from root tips

Dehydrin MsDHN1 improves aluminum tolerance of alfalfa (Medicago sativaL.) by affecting oxalate exudation from root tips

Transcriptomic Analysis Reveals the Contribution of QMrl-7B to Wheat Root Growth and Development

Genome-wide analysis and identification of microRNAs in Medicago truncatula under aluminum stress

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