MSPD Sample Preparation Approach for Reversed-Phase Liquid Chromatographic Analysis of Pesticide Residues in Stem of Coconut Palm

Abstract

A method was developed using matrix solid-phase dispersion, together with liquid chromatography with ultraviolet diode array detector for determination of carbofuran, difenoconazole, β-cyfluthrin, spirodiclofen and thiophanate-methyl in stem of coconut palm. The best results were obtained using 2.0 g of stem, 1.6 g of Florisil as sorbent and cyclohexane:acetone mixture (4:1). The method was validated using stem samples spiked with pesticides at four concentration levels (0.05-2.0 μg/g). Average recoveries ranged from 70 % to 114.3 %, with relative standard deviations between 1.2 % and 19.2 %. Detection and quantification limits were in the ranges 0.02-0.03 and 0.05-0.1 μg/g, respectively.

Multiclass method for the determination of pesticides in stem of coconut palm using matrix solid-phase dispersion prior to liquid chromatography analysis

“…Coconut palm (Cocos nucifera L.) is an important perennial crop palm with high commercial value (Ferreira et al 2013). In Brazil, this palm is used for culinary and industrial purposes; its fruit is consumed raw and also processed into manufactured products and by-products (Brito et al 2002).…”

“…The few methods available for the determination of pesticide residues in wood matrices are mainly based on solid-liquid extraction and final analysis using gas chromatography with mass selective detection (Rasmussen et al 2011;Momohara and Ohmura 2014). Matrix solid-phase dispersion (MSPD) is an extraction method that presents a useful alternative to the traditional extraction techniques employed in the chromatographic analysis of wood extracts (Ferreira et al 2013). MSPD can provide simultaneous sample homogenization, extraction, and cleanup, and requires only small amounts of sample and solvent (Barker 2000(Barker , 2007Santos et al 2012).…”

Evaluation of a novel metal–organic framework as an adsorbent for the extraction of multiclass pesticides from coconut palm (Cocos nucifera L.): An analytical approach using matrix solid‐phase dispersion and liquid chromatography

“…Coconut palm ( Cocos nucifera L.) is one of the major perennial crops with high commercial value . In tropical countries, such as Brazil, this palm has an important role in culinary and industrial purposes .…”

Determination of pesticide residues in coconut tree trunks by modified QuEChERS method and ultra-high-performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry

“…The coconut tree (Cocos nucifera Linn.) 2,3,[6][7][8][9] In the northeastern part of Brazil, different pesticides such as 3-hydroxy-carbofuran, carbendazim, carbofuran, carbosulfan, cyproconazole, difenoconazole, imidacloprid, thiabendazole, thiamethoxam, thiophanate-methyl and spirodiclofen are mixed and used in order to control the pests and diseases in the coconut crop. The application of pesticides is still one of the most used practices to control and prevent pests and diseases.…”

“…However, research involving radiolabeled pesticides involves bureaucratic issues and it is more difficult to perform, because it is necessary to isolate the work area (environmental problem), the number of available radiolabeled pesticides is limited, trained professionals are required, and it is an expensive work due to the controlled acquisition and discarding of the radiolabeled pesticides. This method has many advantages over the method reported by Ferreira et al, 8 which developed the rst methodology for determining ve pesticides applied in treatment of coconut palm trunks using matrix solidphase dispersion (MSPD) with determination by liquid chromatography with an diode array detection (HPLC-DAD). For this a new method of sample preparation for the coconut tree trunk was developed using the "Quick, Easy, Cheap, Effective, Rugged and Safe" (QuEChERS) method for extraction, rst introduced by Anastassiades et al in 2003.…”