MSN‐on‐a‐Chip: Cell‐Based Screenings Made Possible on a Small‐Molecule Microarray of Native Natural Products

Peng, Bo; Du, Shubo; Liew, Si Si; Yuan, Peiyan; Na, Zhenkun; Yao, Shao Q.

doi:10.1002/cbic.201800101

Cited by 11 publications

(5 citation statements)

References 59 publications

(33 reference statements)

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“…Our observation that the aptamer‐based nanovalve system is fully functional in surface‐bound monolayers opens up numerous new fields of application for mesoporous nanoparticle delivery systems. For example, arrays of such monolayers could be used for cell‐based screening of small molecules, as recently demonstrated by printed MSN arrays . Furthermore, since the cargo‐loaded MSN are tethered with the solid surface through stable DNA linkers, the spontaneous ingestion of the container is suppressed, while its cargo can be released on demand to enable diffusion into the cells.…”

Section: Discussionmentioning

confidence: 99%

“…In the case of MSN, DNA‐modification is much better developed due to the fact that capping of MSN with DNA motifs is being utilized to install sophisticated gatekeeper systems that enable stimuli responsiveness and triggered release of encapsulated cargo . Very recently, Peng et al have reported on the top‐down fabrication of spotted microarrays of MSN equipped with photocleavable lids for the light‐triggered release of small molecule cargos . This approach impressively illustrates that surfaces decorated with cargo‐loaded MSN can be used for screening small molecules.…”

Section: Introductionmentioning

confidence: 99%

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Biopebble Containers: DNA‐Directed Surface Assembly of Mesoporous Silica Nanoparticles for Cell Studies

Sun

Leidner

Weigel

et al. 2019

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The development of methods for colloidal self‐assembly on solid surfaces is important for many applications in biomedical sciences. Toward this goal, described is a versatile class of mesoporous silica nanoparticles (MSN) that contain on their surface various types of DNA molecules to enable their self‐assembly into micropatterned surface architectures useful for cell studies. Monodisperse dye‐doped MSN are synthesized by biphase stratification and functionalized with an aptamer oligonucleotide that serves as gatekeeper for the triggered release of encapsulated molecular cargo, such as fluorescent dye rhodamine B or the anticancer drug doxorubicin. One or two additional types of oligonucleotides are installed on the MSN surface to enable DNA‐directed immobilization on solid substrates bearing patterns of complementary capture oligonucleotides. It is demonstrated that this strategy can be used for efficient self‐assembly of microstructured surface architectures, which not only promote the adhesion and guidance of cells but also are capable of affecting the fate of adhered cells through triggered release of their cargo. It is believed that this approach is useful for diverse applications in tissue engineering and nanobio sciences.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biopebble Containers: DNA‐Directed Surface Assembly of Mesoporous Silica Nanoparticles for Cell Studies

Sun

Leidner

Weigel

et al. 2019

Small

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“…1. The sequence starts with the preparation of vanillin derivative 1 following literature-known procedures 53,54 . The compound was then alkylated to introduce the alkyne and gave 2 in good yield (65%).…”

Section: Methodsmentioning

confidence: 99%

NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals

Mougios,

Cotroneo,

Imse

et al. 2024

Preprint

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Fluorescence microscopy has long been a transformative technique in biological sciences. Nevertheless, most implementations are limited to a few targets, revealed using primary antibodies (1.Abs) and fluorescently conjugated secondary antibodies. Super-resolution techniques such as Exchange-PAINT and, more recently, SUM-PAINT have increased multiplexing capabilities, but they require specialized equipment, software, and knowledge. To enable multiplexing for any imaging technique in any laboratory, we developed NanoPlex, a streamlined method based on conventional 1.Abs revealed by engineered secondary nanobodies (2.Nbs) that allow to selectively erase the fluorescence signals. We developed three complementary signal removal strategies: OptoPlex (light-induced), EnzyPlex (enzymatic), and ChemiPlex (chemical). We showcase NanoPlex reaching 21 targets for 3D confocal analyses and 5-8 targets for dSTORM and STED super-resolution imaging. NanoPlex has the potential to revolutionize multi-target fluorescent imaging methods, potentially redefining the multiplexing capabilities of antibody-based assays.

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“…Efforts toward rationale design drug discovery are ongoing, such as natural products inspired fragment based approach [171] and SARS studies [172]. And nontargeted mass spectrometry analyses [173] and other metabolomic based methods (biochemometric) [42] are among several platforms for high-through-put bioassays are being proposed [174,175,176,161,177].…”

Section: Techniques Methods and Approachesmentioning

confidence: 99%

Perspectives on the enhancement of commercially available antibiotics by natural products

Nitsch-Velasquez

Segura²,

Barrios

et al. 2022

Preprint

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Background: Opportunistic resistant bacteria are health and economically relevant in the health care systems and in industries worldwide, especially in the so-called resistant bacteria era (RBE). Enhancing the activity of commercially available antibiotics (CAAs) with di erent types of natural products (NPs) is a successful antimicrobial strategy, for instance the amoxicillin and clavulanate mixture. Objective: To nd research trends in this eld during 2015-2020 and to detect potential drug hits with potential to diversify formulations and materials design that can be useful to manage the RBE. Systematic review results: It yielded 190 reports of synergistic e ects of CAAs and NPs. The analyzed variables were: a) natural products origin: plant family, genera, secondary metabolite type; b) strains: +/- Gram, genera, most frequent species, application eld; and c) CAAs: family, most frequent CAAs. The families with potential to have more bioactive species were Apocynaceae, Rubiaceae, Euphorbiaceae (Isbio factor). Lonicera had the highest reports amount. Polyphenols and avonoids were the majority of pure NPs tested. Several potential drug hits for antibiotic activity enhancement at synergistic level were identi ed together with potential mechanisms of action: berberine (drug e ux inhibitor{DEI, bio lm inhibitor{BI), curcumin (BI), essential oils (BI), 3-o-metyl-butylgallato (inhibition of fatty acid saturation), among others. About the half of the tested strains were gram positive, being Methicillin Resistant Staphylococcus aureus (MRSA) the most frequently tested. Escherichia coli was the gram negative strain most frequently reported, including enterotoxigenic and extended spectrum beta-lactamases producers. The growth of other foodborne genera strains, such as Listeria and Salmonella, were also inhibited. Aminoglycosides were the family most reported, with gentamicin as the most commonly studied. Conclusions: NPs as either as plant extracts from a variety of families, or as puri ed compounds specially avonoids and polyphenols, have shown e ective results to enhance the antibiotic activity of CAAs against gram positive and negative strains relevant to HC and FI. Their mechanisms of action are starting to be determined, as the case EPIs and BIs. Further research is needed to achieve co-formulations and materials design useful for those elds, that can certainly be positively impacted by pursuing this strategy.

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MSN‐on‐a‐Chip: Cell‐Based Screenings Made Possible on a Small‐Molecule Microarray of Native Natural Products

Cited by 11 publications

References 59 publications

Biopebble Containers: DNA‐Directed Surface Assembly of Mesoporous Silica Nanoparticles for Cell Studies

Biopebble Containers: DNA‐Directed Surface Assembly of Mesoporous Silica Nanoparticles for Cell Studies

NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals

Perspectives on the enhancement of commercially available antibiotics by natural products

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