2018
DOI: 10.1002/cbic.201800101
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MSN‐on‐a‐Chip: Cell‐Based Screenings Made Possible on a Small‐Molecule Microarray of Native Natural Products

Abstract: Standard small-molecule microarrays (SMMs) are not well-suited for cell-based screening assays. Of the few attempts made thus far to render SMMs cell-compatible, all encountered major limitations. Here we report the first mesoporous silica nanoparticle (MSN)-on-a-chip platform capable of allowing high-throughput cell-based screening to be conducted on SMMs. By making use of a glass surface on which hundreds of MSNs, each encapsulated with a different native natural product, were immobilized in spatially define… Show more

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Cited by 11 publications
(5 citation statements)
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“…Our observation that the aptamer‐based nanovalve system is fully functional in surface‐bound monolayers opens up numerous new fields of application for mesoporous nanoparticle delivery systems. For example, arrays of such monolayers could be used for cell‐based screening of small molecules, as recently demonstrated by printed MSN arrays . Furthermore, since the cargo‐loaded MSN are tethered with the solid surface through stable DNA linkers, the spontaneous ingestion of the container is suppressed, while its cargo can be released on demand to enable diffusion into the cells.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Our observation that the aptamer‐based nanovalve system is fully functional in surface‐bound monolayers opens up numerous new fields of application for mesoporous nanoparticle delivery systems. For example, arrays of such monolayers could be used for cell‐based screening of small molecules, as recently demonstrated by printed MSN arrays . Furthermore, since the cargo‐loaded MSN are tethered with the solid surface through stable DNA linkers, the spontaneous ingestion of the container is suppressed, while its cargo can be released on demand to enable diffusion into the cells.…”
Section: Discussionmentioning
confidence: 99%
“…In the case of MSN, DNA‐modification is much better developed due to the fact that capping of MSN with DNA motifs is being utilized to install sophisticated gatekeeper systems that enable stimuli responsiveness and triggered release of encapsulated cargo . Very recently, Peng et al have reported on the top‐down fabrication of spotted microarrays of MSN equipped with photocleavable lids for the light‐triggered release of small molecule cargos . This approach impressively illustrates that surfaces decorated with cargo‐loaded MSN can be used for screening small molecules.…”
Section: Introductionmentioning
confidence: 99%
“…1. The sequence starts with the preparation of vanillin derivative 1 following literature-known procedures 53,54 . The compound was then alkylated to introduce the alkyne and gave 2 in good yield (65%).…”
Section: Methodsmentioning
confidence: 99%
“…Efforts toward rationale design drug discovery are ongoing, such as natural products inspired fragment based approach [171] and SARS studies [172]. And nontargeted mass spectrometry analyses [173] and other metabolomic based methods (biochemometric) [42] are among several platforms for high-through-put bioassays are being proposed [174,175,176,161,177].…”
Section: Techniques Methods and Approachesmentioning
confidence: 99%