2016
DOI: 10.1093/chemse/bjv082
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MSG-Evoked c-Fos Activity in the Nucleus of the Solitary Tract Is Dependent upon Fluid Delivery and Stimulation Parameters

Abstract: The marker of neuronal activation, c-Fos, can be used to visualize spatial patterns of neural activity in response to taste stimulation. Because animals will not voluntarily consume aversive tastes, these stimuli are infused directly into the oral cavity via intraoral cannulae, whereas appetitive stimuli are given in drinking bottles. Differences in these 2 methods make comparison of taste-evoked brain activity between results that utilize these methods problematic. Surprisingly, the intraoral cannulae experim… Show more

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Cited by 11 publications
(13 citation statements)
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References 54 publications
(80 reference statements)
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“…This finding suggests that if 5-HT 3 -expressing fibers preferentially convey sour-related information, then these subregions of the nTS should preferentially show responses to sour. and sucrose stimulates neurons more diffusely in the nucleus (Chan et al, 2004;Harrer & Travers, 1996;King, Travers, Rowland, Garcea, & Spector, 1999;Stratford & Thompson, 2016;Travers, 2002;Travers, Herman, Yoo, & Travers, 2007;Travers, Urbanek, & Grill, 1999;. Consistent with our previous findings (Stratford et al, 2017), The presence of acid-induced c-fos activity in the lateral nTS coupled with an abundance of 5-HT 3 -driven GFP is noteworthy in that this portion of the nTS receives little gustatory input as delineated by P2X2 labeling (Bartel, 2012;Ganchrow et al, 2014).…”
Section: Discussionsupporting
confidence: 91%
See 1 more Smart Citation
“…This finding suggests that if 5-HT 3 -expressing fibers preferentially convey sour-related information, then these subregions of the nTS should preferentially show responses to sour. and sucrose stimulates neurons more diffusely in the nucleus (Chan et al, 2004;Harrer & Travers, 1996;King, Travers, Rowland, Garcea, & Spector, 1999;Stratford & Thompson, 2016;Travers, 2002;Travers, Herman, Yoo, & Travers, 2007;Travers, Urbanek, & Grill, 1999;. Consistent with our previous findings (Stratford et al, 2017), The presence of acid-induced c-fos activity in the lateral nTS coupled with an abundance of 5-HT 3 -driven GFP is noteworthy in that this portion of the nTS receives little gustatory input as delineated by P2X2 labeling (Bartel, 2012;Ganchrow et al, 2014).…”
Section: Discussionsupporting
confidence: 91%
“…Regional mapping of taste quality within the nTS in rodents has been demonstrated in previous studies relying on expression of c‐Fos and other immediate‐early genes. In particular, bitter tastants activate medial nTS neurons; MSG and NaCl activate intermediate nTS neurons; and sucrose stimulates neurons more diffusely in the nucleus (Chan et al, ; Harrer & Travers, ; King, Travers, Rowland, Garcea, & Spector, ; Stratford & Thompson, ; Travers, ; Travers, Herman, Yoo, & Travers, ; Travers, Urbanek, & Grill, ; Travers & Travers, ). Consistent with our previous findings (Stratford et al, ), citric acid infusion into the oral cavity of mice activates neurons in lateral and mid sectors of the nTS.…”
Section: Discussionmentioning
confidence: 99%
“…Acid‐evoked neuronal activation was assessed in both WT and P2X‐dbl KO mice. Bilateral intraoral cannulae were implanted into experimental animals as described previously (Stratford and Thompson, ). Briefly, mice were anesthetized with an intramuscular (IM) injection of a combination of medetomidine hydrochloride (Domitor ® ; 0.4 mg/kg; Pfizer, Helsinki, Finland) and ketamine hydrocholoride (40 mg/kg; Bioniche Pharma, Lake Forest, IL).…”
Section: Methodsmentioning
confidence: 99%
“…The NTS receives input from the gustatory neurons and is the first synaptic relay from the peripheral taste system. This approach is commonly used to identify recently activated neurons, including neurons in the taste pathway (King, Garcea et al, 2000, King, Travers et al, 1999, Stratford & Finger, 2011, Stratford & Thompson, 2016, Stratford, Thompson et al, 2017). We reasoned that if BR cells are contributing to the transduction of taste information to the brain, then the loss of PLCβ3 would have a significant impact on the level of c-Fos labeling in the NTS.…”
Section: Resultsmentioning
confidence: 99%