MS2-Affinity Purification Coupled With RNA Sequencing Approach in the Human Pathogen Staphylococcus aureus

Lalaouna, David; Desgranges, Emma; Caldelari, Isabelle; Marzi, Stefano

doi:10.1016/bs.mie.2018.08.022

Cited by 15 publications

(18 citation statements)

References 59 publications

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“…S. epidermidis is the normal microbiota of the nasal surfaces and the newborn umbilicus [9]. S. aureus is an opportunistic bacteria, responsible for nosocomial and community infections [10]. In addition, S. aureus can invade tissues and cause infections such as cutaneous abscesses, endocarditis, and septic shock [10].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial sensitivity patterns of Staphylococcus species isolated from mobile phones and implications in the health sector

et al. 2021

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Objectives The aim of this research was to determine drug sensitivity profiles of Staphylococcus species isolated from mobile phones of students in Microbiology and Biomedical Laboratory Sciences from UZIMA University, Kisumu (Kenya) and the University Colleges Leuven-Limburg, Leuven (Belgium), respectively. Results All mobile phones (16/16, 100%) had gram-positive bacteria. 3/8 (37.5%) mobile devices had Staphylococcus aureus. 2/3 (67%) Staphylococcus aureus strains were resistant to ampicillin, oxacillin, ceftazidime, vancomycin and amoxicillin. Guidelines for disinfection of mobile phones need to be developed urgently to stop transmission of resistant bacteria.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial sensitivity patterns of Staphylococcus species isolated from mobile phones and implications in the health sector

et al. 2021

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“…Crude extracts from Δ rsaC strain expressing MS2-RsaC 544 or from WT strain expressing MS2 tag alone (control) were harvested after 6 h of growth at 37°C in BHI medium. MS2-affinity purifications were performed as previously described (27,28). All steps were performed at 4°C.…”

Section: Methodsmentioning

confidence: 99%

RsaC sRNA modulates the oxidative stress response of Staphylococcus aureus during manganese starvation

Lalaouna

Baude

et al. 2019

Nucleic Acids Research

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The human opportunistic pathogen Staphylococcus aureus produces numerous small regulatory RNAs (sRNAs) for which functions are still poorly understood. Here, we focused on an atypical and large sRNA called RsaC. Its length varies between different isolates due to the presence of repeated sequences at the 5′ end while its 3′ part is structurally independent and highly conserved. Using MS2-affinity purification coupled with RNA sequencing (MAPS) and quantitative differential proteomics, sodA mRNA was identified as a primary target of RsaC sRNA. SodA is a Mn-dependent superoxide dismutase involved in oxidative stress response. Remarkably, rsaC gene is co-transcribed with the major manganese ABC transporter MntABC and, consequently, RsaC is mainly produced in response to Mn starvation. This 3′UTR-derived sRNA is released from mntABC-RsaC precursor after cleavage by RNase III. The mature and stable form of RsaC inhibits the synthesis of the Mn-containing enzyme SodA synthesis and favors the oxidative stress response mediated by SodM, an alternative SOD enzyme using either Mn or Fe as co-factor. In addition, other putative targets of RsaC are involved in oxidative stress (ROS and NOS) and metal homeostasis (Fe and Zn). Consequently, RsaC may balance two interconnected defensive responses, i.e. oxidative stress and metal-dependent nutritional immunity.

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“…S. epidermidis is the normal microbiota of the nasal surfaces and the newborn umbilicus (9). S. aureus is an opportunistic bacteria, responsible for nosocomial and community infections (10). In addition, S. aureus can invade tissues and cause infections such as cutaneous abscesses, endocarditis, and septic shock (10).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Sensitivity Patterns of Staphylococcus Species Isolated from Mobile Phones and Implications in the Health Sector

Likhovole

Achok

Nyaswa

et al. 2020

Preprint

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Objectives: The aim of this research was to determine drug sensitivity profiles of Staphylococcus species isolated from mobile phones of students of Microbiology and Biomedical Laboratory Sciences from UZIMA University of Health and Social Sciences, Kisumu (Kenya) and the University Colleges Leuven-Limburg, Leuven (Belgium), respectively. Results: All mobile phones (16/16,100%) had Gram-positive bacteria. 3/8 (37.5%) mobile devices had Staphylococcus aureus. 2/3 (67%) Staphylococcus aureus strains were resistant to ampicillin, oxacillin, ceftazidime, vancomycin and amoxicillin. Guidelines for disinfection of mobile phones need to be developed urgently to stop transmission of resistant bacteria.

show abstract

MS2-Affinity Purification Coupled With RNA Sequencing Approach in the Human Pathogen Staphylococcus aureus

Cited by 15 publications

References 59 publications

Antimicrobial sensitivity patterns of Staphylococcus species isolated from mobile phones and implications in the health sector

Antimicrobial sensitivity patterns of Staphylococcus species isolated from mobile phones and implications in the health sector

RsaC sRNA modulates the oxidative stress response of Staphylococcus aureus during manganese starvation

Antimicrobial Sensitivity Patterns of Staphylococcus Species Isolated from Mobile Phones and Implications in the Health Sector

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