MrTPS3 and MrTPS20 Are Responsible for β-Caryophyllene and α-Pinene Production, Respectively, in Red Bayberry (Morella rubra)

Wang, Yan; Yang, Qinsong; Zhu, Yifan; Zhao, Lan; Ju, Pengju; Wang, Guoyun; Zhou, Chao-chao; Zhu, Changqing; Jia, Huijuan; Jiao, Yun; Jia, Hao

doi:10.3389/fpls.2021.798086

Cited by 7 publications

(6 citation statements)

References 40 publications

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“…It is clear from the Arabidopsis Cell Electronic Fluorescent Pictograph subcellular localizations profiles that the SoFLDH gene was highly expressed and presented in plasma membrane then endoplasmic reticulum, vacuole, cytosol, mitochondrion, golgi pody and plastid ( Fig 3c ). These results are in line with Ali et al,Taniguchi et al, Chen et al, and Wang et al, [ 26 , 42 – 44 ] who reported that most of TPSs genes were targeted to the plastid or other cell organelles such as mitochondrion and nucleus.…”

Section: Resultssupporting

confidence: 90%

Molecular characterization of a Novel NAD+-dependent farnesol dehydrogenase SoFLDH gene involved in sesquiterpenoid synthases from Salvia officinalis

et al. 2022

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Salvia officinalis is one of the most important medicinal and aromatic plants in terms of nutritional and medicinal value because it contains a variety of vital active ingredients. Terpenoid compounds, particularly monoterpenes (C10) and sesquiterpenes, are the most important and abundant among these active substances (C15). Terpenes play a variety of roles and have beneficial biological properties in plants. With these considerations, the current study sought to clone theNAD+-dependent farnesol dehydrogenase (SoFLDH, EC: 1.1.1.354) gene from S. officinalis. Functional analysis revealed that, SoFLDH has an open reading frame of 2,580 base pairs that encodes 860 amino acids.SoFLDH has two conserved domains and four types of highly conserved motifs: YxxxK, RXR, RR (X8) W, TGxxGhaG. However, SoFLDH was cloned from Salvia officinalis leaves and functionally overexpressed in Arabidopsis thaliana to investigate its role in sesquiterpenoid synthases. In comparison to the transgenic plants, the wild-type plants showed a slight delay in growth and flowering formation. To this end, a gas chromatography-mass spectrometry analysis revealed that SoFLDH transgenic plants were responsible for numerous forms of terpene synthesis, particularly sesquiterpene. These results provide a base for further investigation on SoFLDH gene role and elucidating the regulatory mechanisms for sesquiterpene synthesis in S. offcinalis. And our study paves the way for the future metabolic engineering of the biosynthesis of useful terpene compounds in S. offcinalis.

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Section: Resultssupporting

confidence: 90%

Molecular characterization of a Novel NAD+-dependent farnesol dehydrogenase SoFLDH gene involved in sesquiterpenoid synthases from Salvia officinalis

et al. 2022

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“…Moreover, SgCINS was reported to be localized in the plastid (Supplementary Figure 2C). These results are in line with studies by Taniguchi et al (2014), Chen et al (2018), Ali et al (2021), Wang et al (2022) who reported that most of TPSs genes were targeted to the plastid or other cell organelles such as mitochondrion and nucleus. Additionally, an organ-specific expression pattern of SgCINS was determined using mRNA samples of 2-year-old S. guaranitica plants using the qRT-PCR.…”

Section: Discussionsupporting

confidence: 92%

Cloning and Characterization of 1,8-Cineole Synthase (SgCINS) Gene From the Leaves of Salvia guaranitica Plant

et al. 2022

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Monoterpenes are one of the most common groups belonging to the terpenoid family, with a C10 structure comprising of two isoprene units. Most of monoterpenes are volatile plant compounds, and they act as signaling molecules between plants and the environment, particularly as defensive compounds against herbivores and pathogens. In this study, 1,8-cineole synthase (SgCINS) gene was identified and cloned from the leaves of Salvia guaranitica plant. To examine the role of SgCINS in insect resistance, we transformed and expressed this gene into tobacco leaves. The metabolic analysis revealed that the production of various types and amount of terpenoid was increased and decreased in SgCINS overexpression and control lines, respectively, suggesting that overexpressing SgCINS in transgenic tobacco plants lead to an increase in the production of various types of terpenoids and other phytochemical compounds. These results indicated why transgenic tobacco was highly resistant against cotton worm than the highly susceptible control plants. Our results demonstrate that the SgCINS gene can play an important role in plants against cotton worm insect attack, and pave the way for using terpenoids genes for improving resistance to insect attack in higher plants.

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“…3c). These results are in line with Taniguchi et al (2014), Chen et al (2018), Ali et al (2021) and Wang et al (2022). They reported that most of TPSs genes were targeted to the cytosol or other cell organelles such as plastid, mitochondrion and nucleus.…”

Section: Soamys Genesupporting

confidence: 82%

MOLECULAR CLONING AND CHARACTERIZATION OF BETA-AMYRIN SYNTHASE (SoAMYS) GENE FROM SALVIA OFFICINALIS PLANT

El-ramah

Ali

Elsherbeny

et al. 2022

Egyptian Journal of Desert Research

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erpenes (terpenoids or isoprenoids) play a key role in primary and secondary metabolism in a variety of organisms. In plants, its biosynthesis is catalyzed by two key pathways mevalonate and non-mevalonate pathways. The genome of garden sage plant (Salvia officinalis) contains 65 terpene synthase (SoTPS) genes, and only a few genes which related to terpenoids were studied. Here, we demonstrate the functional characterization of beta-amyrin synthase (SoAMYS) gene, by introducing it into Arabidopsis thaliana and found that SoAMYS overexpression improved the flowers formation in transgenic Arabidopsis compared to the wild type plants. Metabolic analysis reported that the production of various types of terpenes, especially beta-amyrin triterpene which, were increased and decreased in SoAMYS overexpression and wild type lines, respectively. These finding suggesting that SoAMYS functions as a beta-amyrin synthase in plant. Our results were further supported using some bioinformatics tools to predict the putative subcellular localization and tissue-specific expression of SoAMYS. In context that, SoAMYS gene was reported to be localized in plastids with highly expression level in flowers stage 15, pedicels. This is the first report of a gene involved in the beta-amyrin as a triterpene from S. officinalis plant.

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MrTPS3 and MrTPS20 Are Responsible for β-Caryophyllene and α-Pinene Production, Respectively, in Red Bayberry (Morella rubra)

Cited by 7 publications

References 40 publications

Molecular characterization of a Novel NAD+-dependent farnesol dehydrogenase SoFLDH gene involved in sesquiterpenoid synthases from Salvia officinalis

Molecular characterization of a Novel NAD+-dependent farnesol dehydrogenase SoFLDH gene involved in sesquiterpenoid synthases from Salvia officinalis

Cloning and Characterization of 1,8-Cineole Synthase (SgCINS) Gene From the Leaves of Salvia guaranitica Plant

MOLECULAR CLONING AND CHARACTERIZATION OF BETA-AMYRIN SYNTHASE (SoAMYS) GENE FROM SALVIA OFFICINALIS PLANT

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