MRSA USA300 Clone and VREF — A U.S.–Colombian Connection?

Arias, César A.; Rincón, Sandra; Chowdhury, Shahreen; Martínez, Ernesto; Coronell, Wilfrido; Reyes, Jinnethe; Nallapareddy, Sreedhar R.; Murray, Barbara E.

doi:10.1056/nejmc0804021

Cited by 60 publications

(73 citation statements)

References 5 publications

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“…PVL-positive CC8-MSSA was abundant in Trinidad and Tobago, although this strain was rare elsewhere. This lends credence to the hypothesis that the USA300 strain emerged in the Caribbean/Latin American region (32).…”

Section: Discussionsupporting

confidence: 71%

Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies

Monecke

Müller

Buechler³

et al. 2013

J Clin Microbiol

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Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spotted on protein microarrays and screened using different lukF-PV preparations and detection antibodies. This led to the identification of the optimal antibody combination that was then used to establish a lateral flow assay. This test was used to detect PVL in S. aureus cultures. The detection limit of the assay with purified native and recombinant antigens was determined to be around 1 ng/ml. Overnight cultures from various solid and liquid media proved suitable for PVL detection. Six hundred strains and clinical isolates from patients from America, Europe, Australia, Africa, and the Middle East were tested. Isolates were genotyped in parallel by DNA microarray hybridization for confirmation of PVL status and assignment to clonal complexes. The sensitivity, specificity, and positive and negative predictive values of the assay in this trial were 99.7, 98.3, 98.4, and 99.7%, respectively. A total of 302 clinical isolates and reference strains were PVL positive and were assigned to 21 different clonal complexes. In summary, the lateral flow test allows rapid and economical detection of PVL in a routine bacteriology laboratory. As the test utilizes cultures from standard media and does not require sophisticated equipment, it can be easily integrated into a laboratory's workflow and might contribute to timely therapy of PVLassociated infections.

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Section: Discussionsupporting

confidence: 71%

Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies

Monecke

Müller

Buechler³

et al. 2013

J Clin Microbiol

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“…While analyzing infections caused by PVL ϩ S. aureus in Spain, Blanco and colleagues found two isolates with spa type t1635: one methicillinsusceptible S. aureus (MSSA) strain and one MRSA strain, the latter having molecular characteristics similar to those of the new clone detected (ST8-MRSA-IVa; PVL positive and ACME negative) and being genetically unrelated to USA300 (11). In Latin American countries, such as Colombia and Venezuela, some related isolates have been found; however, they were not identified as having a spa type, an agr group, or the insertion in the sausa300_0808 gene found in SaPI5, and those isolates show some differences in PFGE pulsotype (2,12).…”

mentioning

confidence: 73%

Detection of a New Community Genotype Methicillin-Resistant Staphylococcus aureus Clone That Is Unrelated to the USA300 Clone and That Causes Pediatric Infections in Colombia

et al. 2013

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dThe dissemination of a clone of community genotype methicillin-resistant Staphylococcus aureus (CG-MRSA) that is related to USA300 has been reported in Latin America. We recently detected isolates of a new clone of CG-MRSA (spa type t1635 and ACME-negative) that was genetically unrelated to the USA300 clone and that causes infections in children in Colombia. This finding indicates the appearance of a new clone of CG-MRSA in our region.

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“…This result can be found online in an earlier investigation by Tacconelli, et al, which identified the association of this family of antibiotics with the emergence of resistant microorganisms in hospitals, indicating patterns of consuming broad spectrum antimicrobials which should be reviewed locally (24). The indiscriminate use of antibiotics has been described as a factor that exerts selection pressure on S. aureus resistant clones in the health-care setting (8).…”

Section: Discussionmentioning

confidence: 77%

“…We did not perform the molecular definition of the type of isolations and their relationships with community-acquired infection in the present investigation. Some reports in Colombia have described the presence of USA 300 clones related with SCC mec type IV genes in patients with multisensitive MRSA infection (8,23), which changed the predominant clone towards the end of the 2000-2010 decade (22,26).…”

Section: Discussionmentioning

confidence: 99%

Risk factors for methicillin-resistant Staphylococcus aureus bacteremia: A multicenter matched case-control study

et al. 2016

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Introduction: Methicillin-resistant Staphylococcus aureus is a frequent pathogen at critical care services. Its presence leads to increased hospital stays and mortality risk in patients with bacteremia. However, the etiology of this resistance marker has not been fully studied. Objective: To identify risk factors associated with the emergence of methicillin-resistant S. aureus bacteremia in critically ill patients treated at intensive care units in Bogotá, Colombia. Materials and methods:We conducted a retrospective paired case-control study, nested in a cohort of patients diagnosed with S. aureus bacteremia and treated at intensive care units between 2006 and 2008 in Bogotá. Cases were patients with positive blood culture to methicillin resistance, matched in a 1:1 ratio with methicillin-sensitive controls isolated from the same institution and hospitalization year. We used conditional logistic regression to analyze the risk factors associated with the presence of resistance, with emphasis on prior antibiotic therapy. Results: We included 372 patients with S. aureus bacteremia. Factors such as the use of pre-hospital devices: vascular (OR=1.986, 95% CI 1.038 to 3.801) and urinary (OR=2.559, 95% CI: 1.170 to 5.596), along with the number of previously used antibiotics, were associated with the emergence of resistance. The number of antibiotics used previously was determined to have a gradient effect, particularly carbapenems. Conclusions:The rational use of antibiotics and surveillance of exposure to surgical procedures or use of invasive devices are interventions that could diminish the emergence of methicillin-resistant S. aureus bacteremia causes. Introducción. Staphylococcus aureus resistente a la meticilina es uno de los agentes patógenos más frecuentes en las unidades de cuidados intensivos. Su presencia prolonga las hospitalizaciones y aumenta el riesgo de mortalidad en los pacientes con bacteriemia. Sin embargo, la etiología de este marcador de resistencia no ha sido completamente estudiada. Objetivo. Determinar los factores asociados con la aparición de S. aureus resistente a la meticilina causante de bacteriemia en pacientes atendidos en unidades de cuidados intensivos en Bogotá. Materiales y métodos. Se hizo un estudio retrospectivo de casos y controles emparejados, anidado en una cohorte de pacientes con diagnóstico de bacteriemia por S. aureus atendidos en unidades de cuidados intensivos de Bogotá entre 2006 y 2008. Los casos fueron pacientes con hemocultivo positivo para resistencia a la meticilina, emparejados 1 a 1 con controles con hemocultivos sensibles 613 Conclusiones. El uso racional de antibióticos y la vigilancia de la exposición a procedimientos quirúrgicos o al uso de dispositivos invasivos, son intervenciones que podrían disminuir la aparición de S. aureus resistente a meticilina causante de bacteriemia.Palabras clave: Staphylococcus aureus resistente a meticilina; factores de riesgo; farmacorresistencia microbiana; unidades de cuidados intensivos; estudios de casos y controles.

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MRSA USA300 Clone and VREF — A U.S.–Colombian Connection?

Cited by 60 publications

References 5 publications

Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies

Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies

Detection of a New Community Genotype Methicillin-Resistant Staphylococcus aureus Clone That Is Unrelated to the USA300 Clone and That Causes Pediatric Infections in Colombia

Risk factors for methicillin-resistant Staphylococcus aureus bacteremia: A multicenter matched case-control study

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