MRSA in swine, farmers and abattoir workers in Southern Italy

Parisi, Antonio; Caruso, Marta; Normanno, G.; Latorre, Laura; Miccolupo, Angela; Fraccalvieri, Rosa; Intini, Francesco; Manginelli, Teresa; Santagada, Gianfranco

doi:10.1016/j.fm.2019.03.003

Cited by 32 publications

(36 citation statements)

References 58 publications

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“…Regarding environmental surfaces, the studies performed in veterinary hospitals [11] and households [10] showed rates of 0% and 46% in MRSA isolates, respectively. The airborne dust and unsterilized medical device also constituted the risk of spreading S. aureus [36,37]. The present study indicated the presence of S. aureus in medical device surfaces, environmental surfaces, and airborne dust from veterinary hospitals, and S. aureus in airborne dust was the most prevalent (19.1%).…”

Section: Discussionmentioning

confidence: 54%

Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in Staphylococcus aureus from Various Veterinary Hospitals

et al. 2020

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Staphylococcus aureus (S. aureus) is one of the most clinically important zoonotic pathogens, but an understanding of the prevalence, biofilm formulation ability, virulence, and antimicrobial resistance genes of S. aureus from veterinary hospitals is lacking. By characterizing S. aureus in different origins of veterinary hospitals in Guangzhou, China, in 2019, we identified with the presence of S. aureus in pets (17.1%), veterinarians (31.7%), airborne dust (19.1%), environmental surfaces (4.3%), and medical device surfaces (10.8%). Multilocus sequence typing (MLST) and Staphylococcus protein A (spa) typing analyses demonstrated methicillin-sensitive S. aureus (MSSA) ST398-t571, MSSA ST188-t189, and methicillin-resistant S. aureus (MRSA) ST59-t437 were the most prevalent lineage. S. aureus with similar pulsed-field gel electrophoresis (PFGE) types distributed widely in different kinds of samples. The crystal violet straining assays revealed 100% (3/3) of MRSA ST59 and 81.8% (9/11) of MSSA ST188 showed strong biofilm formulation ability, whereas other STs (ST1, ST5, ST7, ST15, ST88, ST398, ST3154 and ST5353) showed weak biofilm production ability. Polymerase chain reaction (PCR) confirmed the most prevalent leucocidin, staphylococcal enterotoxins, ica operon, and adhesion genes were lukD-lukE (49.0%), sec-sel (15.7%), icaA-icaB-icaC-icaR (100.0%), and fnbB-cidA-fib-ebps-eno (100.0%), respectively. Our study showed that the isolates with strong biofilm production ability had a higher prevalence in clfA, clfB, fnbA and sdrC genes compared to the isolates with weak biofilm production ability. Furthermore, 2 ST1-MRSA isolates with tst gene and 1 ST88-MSSA isolate with lukS/F-PV gene were detected. In conclusion, the clonal dissemination of S. aureus of different origins in veterinary hospitals may have occurred; the biofilm production capacity of S. aureus is strongly correlated with ST types; some adhesion genes such as clfA, clfB, fnbA, and sdrC may pose an influence on biofilm production ability and the emergence of lukS/F-PV and tst genes in S. aureus from veterinary hospitals should raise our vigilance.

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Section: Discussionmentioning

confidence: 54%

Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in Staphylococcus aureus from Various Veterinary Hospitals

et al. 2020

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“…Similar findings were noted in Iranian hospital food workers who reported that all the Staphylococcus strains resist to penicillins (100%) and showed high resistance rates to ofloxacin (88%), tobramycin (73·90%), ciprofloxacin (70%) and cefoxitin (50%) (Safarpoor Dehkordi et al ), while in China O'Donoghue et al noted a very low percentage of resistance against Penicillin GG among food workers (1·1%) (Ho et al ). In general, the highest rates of resistance associated to nasal carriage worldwide are reported among the food handlers exposed to livestock or raw meats (Hoet et al ; Osman et al ; Fetsch ; Parisi et al ; Wu et al ).…”

Section: Resultsmentioning

confidence: 99%

Prevalence and antibiotic resistance profiles ofStaphylococcussp. isolated from food, food contact surfaces and food handlers in a Moroccan hospital kitchen

Touimi

Bennani

Berrada

et al. 2020

Lett Appl Microbiol

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Food poisoning risk related to the consumption of contaminated food with known foodborne pathogens or antibiotic‐resistant bacteria is currently a serious threat for public health. Thus, pathogenic methicillin‐resistant Staphylococcus strains are considered as one of the major cause of foodborne diseases in hospitals. The present study aims to determine the prevalence and the antibiotic resistance patterns of Staphylococcus in various types of hospital food samples, work surfaces and its carriage by food handlers. A total of 608 collected samples including 300 food samples, 238 food contact surfaces and 70 nasal and hand samples were tested. The identified Staphylococcus and their antibiotic resistance patterns were analysed using the agar disk‐diffusion and PCR method was used for mecA resistance gene amplification. The prevalence of S. aureus and the coagulase‐negative staphylococci were 17·33 and 23·33%, respectively. The antibiotic resistance reached 100% towards oxacillin and Penicillin G for both S. aureus and CoNs. The mecA gene was detected in 5·71% (4/70) and 7·69% (4/52) of S. aureus and CoNs strains, respectively. The outcome of this study enlightens isolation of MRSA strains and resistant CoNs from food, food contact surfaces and food handlers. The presence of this resistant species in this critical setting, where products were intended to vulnerable and immunocompromised patients, represents a serious threat to this community. It can be a source of nosocomial infection and more precautions must be taken to prevent staphylococci food contamination mainly in hospitals. Significance and Impact of the Study This is the first study describing the antibiotic resistance patterns of Staphylococcus aureus and coagulase‐negative Staphylococcus isolated from hospital food, food contact surfaces and food handlers samples in a Moroccan hospital kitchen. High levels of multi‐resistance were reported. The alarming outcome of this study emphasizes the crucial need of implementing an approach to fight multidrug‐resistant staphylococci mainly in healthcare settings, where the community have already compromised health issues.

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“…Pig herds are an important reservoir for MRSA, with ST398 representing the most frequently reported subtype at European level in swine production chain (EFSA, 2009a; Battisti et al , 2010 ; Parisi et al , 2019 ). Although pig colonization with MRSA ST398 is frequent, infection in swine is rare.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of methicillin-resistant <em>Staphylococcus aureus</em> isolated from the pig production chain in Northern Italy

et al. 2020

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This study aimed to evaluate the molecular characteristics of methicillin resistant Staphylococcus aureus (MRSA) isolated in the swine chain in Northern Italy. A sample of 50 fattening units located in Lombardy was selected. Five cutaneous samples at slaughtering and three environmental samples at farm were collected from each unit giving a total of 250 and 150 samples, respectively. A total of 25 MRSA isolates were isolated from 400 samples, in 17 different fattening units. At farm, 12 out of 250 samples were positive for MRSA (4,8 %), and 13 out of 150 samples at slaughter were identified as MRSA (8,7 %), giving an overall incidence among samples of 6,25 % (n = 25). Molecular characterization was carried out using multi-locus sequence typing (MLST) and spa-typing. Outcomes showed that most of the isolates belonged to ST398, carrying spa-types t899, t011, t18498, t1939, t1200, and t304. Nonetheless, three isolates were identified as ST97 (t1730 and t4795), and one as ST30, showing spa-type t318. Furthermore, a novel ST was identified, namely 5422, showing spa-type t1730. Heterogeneity in genotypes within the same farm was also found in different fattening units, with concern for the possibility of the exchange of genetic determinants among different lineages. Genetic diversity among MRSA isolates in pig fattening units has been observed, highlighting the possibility that some isolates could be able to infect different hosts, including human.

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MRSA in swine, farmers and abattoir workers in Southern Italy

Cited by 32 publications

References 58 publications

Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in Staphylococcus aureus from Various Veterinary Hospitals

Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in Staphylococcus aureus from Various Veterinary Hospitals

Prevalence and antibiotic resistance profiles ofStaphylococcussp. isolated from food, food contact surfaces and food handlers in a Moroccan hospital kitchen

Molecular characterization of methicillin-resistant <em>Staphylococcus aureus</em> isolated from the pig production chain in Northern Italy

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