MRSA detection in raw milk, some dairy products and hands of dairy workers in Egypt, a mini-survey

Kamal, Rania M.; Bayoumi, Mohamed A.; Aal, S. F. A. Abd El

doi:10.1016/j.foodcont.2013.02.017

Cited by 61 publications

(55 citation statements)

References 69 publications

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“…There was no statistical difference among the food groups in terms of enterotoxigenic S. aureus presence. There is a lot of research reporting various rates of S. aureus and/or enterotoxigenic S. aureus isolation in food (Aydin et al 2011b;Can and Çelik 2012;Carfora et al 2015;Kamal et al 2013;Vazquez-Sanchez et al 2012). There are many reasons behind these varying rates expressed in the study results, such as the sensitivity of analysis methods, the food process being analyzed, selling conditions and the form of presentation to consumption.…”

Section: Discussionmentioning

confidence: 99%

“…in food handlers and the studies performed indicate that different amounts of Staphylococcus spp. can be found on mouths, noses and hands of food handlers (Asghar et al 2006;El-Shenawy et al 2013;Ho et al 2014;Kamal et al 2013;Uzunoviç et al 2013). Differences in results could be based on various factors such as health conditions of food handlers, their immune systems, cleaning habits, level of education, the state of raw-cooked food processing, the hygiene of utensils-equipment and the environment, the state of development of the country the food handler is working in and the regulations on inquest (El-Shenawy et al 2013;Ho et al 2014;Sivaraman et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In the studies conducted on toxin genes of enterotoxigenic S. aureus, it was determined that a strain usually contained one or more toxin genes. In particular sea-see-genes were investigated by various different methods like PCR or ELISA, and other toxin genes and their genotypes were detected (Carfora et al 2015;El-Shenawy et al 2013;Kamal et al 2013). The results from other studies (Ho et al 2014;Omoe et al 2002;Schlegelova et al 2010;Vazquez-Sanchez et al 2012) confirm our results showing that enterotoxigenic S. aureus can be commonly found on hands, noses and mouths of food handlers, on surfaces that are in contact with food, utensils and equipment, and that this may exhibit a serious public health concern.…”

Section: Discussionmentioning

confidence: 99%

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Food handlers: a bridge in the journey of enterotoxigenic MRSA in food

et al. 2015

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We investigated the presence of enterotoxigenic and methicillin-resistant Staphylococcus aureus (MRSA) in a kitchen of a catering firm by collecting 100 food samples and 100 utensil-equipment samples after sanitation. Additional 186 samples in total were collected by swabbing the mouths, noses and hands of 62 kitchen staff members during working hours. The isolation of S. aureus was performed using a culture method and verified by using a genetic method (PCR). The presence of toxin genes and the toxic shock syndrome gene (tst) was analyzed by PCR. ELISA was used to investigate whether the isolates bearing a toxin gene that produces enterotoxin or not. In this research we obtained 1930 isolates from 386 samples. Using molecular typing by PCR, 842 Staphylococcus spp. were found of which 307 (36.4 %) were identified as S. aureus. 198 (64.4 %) of the S. aureus strains were identified as CoA positive and 89 (28.9 %) as MRSA. Our data demonstrate the presence of MRSA and enterotoxigenic S. aureus in a catering kitchen providing daycare and hospital refectories. Given the facts that the rate of kitchen staff members contaminated with enterotoxigenic strains was rather high and that the kitchen contained S. aureus even after sanitation and that there was uneducated staff and insufficient hygiene practices aroused suspicions of the presence of biofilms and/or multi-resistant strains which could manifest a serious public health concern.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Food handlers: a bridge in the journey of enterotoxigenic MRSA in food

et al. 2015

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“…As condições de crescimento são praticamente as mesmas nesses meios de cultura, incubação na faixa de 35-37°C por 24/48 horas, sob aerobiose. Antes do plaqueamento pode ser realizado um pré-enriquecimento em meios líquidos como caldo Muller Hinton contendo 6,5% de NaCl e/ou caldo triptona de soja contendo cefoxitina (3,5mg/L) (Kamal et al 2013, Lim et al 2013, Chandrasekaran et al 2014b, Vishnupriya et al 2014. Após o crescimento das colônias podem ser realizadas provas bioquímicas como teste da catalase, coagulase e fermentação da glicose para diferenciação das espécies de estafilococos (Silva et al1997).…”

Section: Staphylococcus Spp Resistente à Meticilina Em Casos De Masunclassified

Mastite bovina causada por Staphylococcus spp. resistentes à meticilina: revisão de literatura

2018

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RESUMO.-Staphylococcus spp. são os micro-organismos mais relacionados a casos de mastite bovina. Algumas cepas destes micro-organismos têm apresentado fatores de virulência como genes de resistência a antimicrobianos com destaque para a resistência à meticilina que é um problema de saúde pública. Esta revisão de literatura tem o objetivo de compilar dados sobre a mastite bovina causada por Staphylococcus spp. resistente à meticilina (MRS). Apesar desse antimicrobiano não ser comumente utilizado no tratamento das mastites, a frequência de casos de infecção da glândula mamária causada por MRS tem variado entre 1,34 a 47,6%. Acredita-se que o contato dos humanos com animais positivos para MRS e vice-versa favoreça a transmissão deste patógeno entre as espécies, contribuindo para a variação nas taxas de infecção. A detecção de MRS pode ser realizada por meio de provas fenotípicas, moleculares ou sorológicas e as medidas de controle devem contemplar a identificação dos casos, segregação dos animais, estudo epidemiológico da fonte de infecção do rebanho, além da constante limpeza e higienização do ambiente de confinamento, equipamentos e utensílios de ordenha. Casos de mastite ocasionados por esse patógeno assumem relevância para a saúde pública, pois a ingestão de leite e/ou derivados contaminados podem desencadear a transferência de MRS para seres humanos. Com isso, é necessário um alerta constante quanto à vigilância epidemiológica em fazendas leiteiras. The most related microorganism in cases of bovine mastitis are Staphylococcus spp. Some strains of these microorganisms have shown virulence factors like antibiotic resistance genes, such as the resistance to methicillin, which represents a public health problem. This literature review aims to compile data related to bovine mastitis caused by Staphylococcus spp. Methicillin-resistant (MRS). Despite this antimicrobial not be commonly used in the treatment of mastitis, the frequency of cases of infection of the mammary gland caused by MRS has ranged from 1.34 to 47.6%. It is believed that the contact of humans with animals positive for MRS and vice versa favors the transmission of this pathogen among species, contributing to the variation in infection rates. MRS detection can be performed by phenotypic tests, molecular tests or serological tests and control measures must be taken such as the identification of cases, animal segregation, epidemiological study of the infection source of herd and the constant cleanliness and hygiene of the confined environment, equipment and milking utensils. Mastitis cases caused by this pathogen are of great relevance to public health because the ingestion of contaminated and/or derived from milk may trigger the transfer of MRS for human. Thus, a constant warning is required on the epidemiological surveillance in dairy farms.

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“…Other potential sources of ABR are the manure and grey waters from treated cattle and other animals. Among the antimicrobial-resistant pathogens, methicillin-resistant Staphylococcus aureus (MRSA) [48,49] and vancomycin-resistant Enterococcus spp. (VRE) [47,50] are some of the most investigated.…”

Section: Antibiotic-resistant Bacteriamentioning

confidence: 99%

Conventional and New Processes for Urban Wastewater Disinfection: Effect on Emerging and Resistant Microorganisms

Ferro

Polo-López

Fernández‐Ibáñez

2015

The Handbook of Environmental Chemistry

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The continuous release of chemical and microbiological pollutants into the environment and the increasing demand for safe water call for effective water and wastewater treatment processes. In particular, the detection of resistant microorganisms (e.g. antibiotic-resistant bacteria) in the effluents of urban wastewater treatment plants disposed into surface water or reused (e.g. in crop irrigation) shows that conventional treatments and disinfection processes do not effectively control the spread of pathogens into the environment. There is a need for new and more effective disinfection processes and technologies. The aim of this chapter is to briefly describe some of the emerging and antimicrobial-resistant microorganisms detected in wastewater, as well as the conventional and new advanced available technologies for wastewater disinfection, and to evaluate and discuss their effect on these microorganisms. Moreover, regulations and policies on wastewater reuse are also critically discussed and compared.

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MRSA detection in raw milk, some dairy products and hands of dairy workers in Egypt, a mini-survey

Cited by 61 publications

References 69 publications

Food handlers: a bridge in the journey of enterotoxigenic MRSA in food

Food handlers: a bridge in the journey of enterotoxigenic MRSA in food

Mastite bovina causada por Staphylococcus spp. resistentes à meticilina: revisão de literatura

Conventional and New Processes for Urban Wastewater Disinfection: Effect on Emerging and Resistant Microorganisms

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