MRSA and multidrug-resistant Staphylococcus aureus in U.S. retail meats, 2010–2011

Ge, Beilei; Mukherjee, Sampa; Hsu, Chih-Hao; Davis, Johnnie A.; Tran, Thu Thuy T.; Yang, Qianru; Abbott, Jason; Ayers, Sherry; Young, Shenia; Crarey, Emily; Womack, Niketta; Zhao, Shaohua; McDermott, Patrick F.

doi:10.1016/j.fm.2016.10.029

Cited by 85 publications

(84 citation statements)

References 37 publications

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“…Given that the food chain is indispensable to human society, the assumption that contaminated food products may serve as vehicles for the spread of pathogens has been widely recognized (Ogata et al, 2012;Schoder et al, 2015;Oniciuc et al, 2017). However, most of the previous studies regarding MRSA in the food chain have only characterized the relationship between multiple bacterial isolates using multi-locus sequence typing (MLST) (Ge et al, 2017;Rodriguez-Lazaro et al, 2017;Rong et al, 2017;Tang et al, 2017;Wu et al, 2019), a method that is dependent on housekeeping genes. Thus, the genetic similarity of housekeeping genes cannot exclude the possibility that these isolates are derived from ancestors separated by earlier divergence events rather than by direct food chain-transmission.…”

Section: Discussionmentioning

confidence: 99%

The Genomic Context for the Evolution and Transmission of Community-Associated Staphylococcus aureus ST59 Through the Food Chain

Pang

Zhang

et al. 2020

Front. Microbiol.

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Sequence type 59 (ST59) is a predominant clonal lineage of community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) in Asia. Despite its increasing clinical relevance in China, the evolution and geographic expansion of ST59 has been relatively uncared for. Previous study has shown that ST59 was the predominant clone in food-related MRSA in China. This study compared the genomes of 87 clonal complex (CC) 59 S. aureus isolates sourced from food chain and infection cases to reconstruct the molecular evolution and geographical spread of ST59. Accordingly, three major sub-clades of ST59 were identified and these did not correlate with isolation source or location. Phylogenetic analysis estimated that ST59 in mainland China diverged from a most common recent ancestor around 1974, and most of the cases of cross-country transmission occurred between 1987 and 2000. Notably, two recent events of crosscountry transmission through the food chain were observed, the isolates from these events diverged within relatively short time intervals. These isolates also showed high similarity in terms of their core genome, accessory genes, and antibiotic resistance patterns. These findings provide a valuable insight into the potential route of ST59 expansion in China and indicate a need for robust food chain surveillance to prevent the spread of this pathogen.

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Section: Discussionmentioning

confidence: 99%

The Genomic Context for the Evolution and Transmission of Community-Associated Staphylococcus aureus ST59 Through the Food Chain

Pang

Zhang

et al. 2020

Front. Microbiol.

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“…It is a common causal pathogen of skin abscesses, pharyngitis, sinusitis, meningitis, pneumonia, osteomyelitis, endocarditis, toxic shock syndrome, sepsis, and wound infections following surgery [ 3 ]. It is also responsible for food poisoning illness as it is capable of producing several virulence factors such as enterotoxins, adhesins, hemolysins, invasins, superantigens, and surface factors that inhibit its phagocytic engulfment [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Staphylococcus aureus LC 554891 by Moringa oleifera Seed Extract either Singly or in Combination with Antibiotics

Enan

Al-Mohammadi²,

Mahgoub

et al. 2020

Molecules

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Bacterial outbreaks caused by Staphylococcus aureus (S. aureus) are interesting due to the existence of multidrug resistant (MDR) isolates. Therefore, there is a need to develop novel ways to control such MDR S. aureus. In this study, some natural agents such as honey bee (HB), extracts of either Moringa oleifera seeds (MSE), or leaves (MLE) and essential oils of garlic, clove, and moringa were studied for their inhibitory activity against this S. aureus pathogen. About 100 food samples including beef luncheon (n = 25), potato chips (n = 50), and corn flakes (n = 25) were investigated for possible pollution with the S. aureus bacteria. The isolated bacteria suspected to belong S. aureus that grew well onto Baird–Parker agar (Oxoid) and shiny halo zones and positive coagulase reaction were selected and identified by API-Kits; all of them that were approved belong to S. aureus (18 strains). The sensitivity of the obtained 18 S. aureus bacterial strains to 12 antibiotics were evaluated; all of them were resistant to ofloxacin; however, other antibiotics tested showed variable results. Interestingly, the S. aureus No. B3 isolated from beef luncheon was resistant to10 antibiotics out of 12 ones tested. Multiple antibiotic resistance index (MAR) of this S. aureus strain was about 83.3%. Therefore, its identification was confirmed by sequencing of a 16S rRNA gene which approved a successful biochemical identification carried out by API Kits and such strain was designated S. aureus LC 554891. The genome of such strain appeared to contain mecA gene encoding methicillin resistance; it was found to contain hla, hlb, tsst-1, and finbA that encode α-blood hemolysis, β-blood hemolysis, toxic shock syndrome gene, and fibrinogen-binding protein gene, respectively. In addition, the virulence factors viz. sea; seb; sec encoding enterotoxins were detected in the DNA extracted from S. aureus B3 strain. Aqueous extract of Moringa oleifera seeds (MSE) showed inhibitory activity against S. aureus LC 554891 better than that obtained by tetracycline, essential oils or HB. Minimum inhibitory concentration (MIC) of MSE was 20µg/mL. Instrumental analysis of MSE showed 14 bioactive chemical compounds. Combinations of both MSE and tetracycline showed distinctive inhibitory activity against S. aureus LC 554891 than that obtained by either tetracycline or MSE singly.

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“…The role of meat as a reservoir of MRSA strains was also determined in Australia, 28 the United Kingdom 29 and the United States. 30 Our findings revealed that chicken and, in some cases, turkey may be the sources of MRSA strains. On the contrary, quail and especially ostrich were not permanent sources of MRSA strains.…”

Section: Discussionmentioning

confidence: 57%

Prevalence of Staphylococcal Cassette Chromosome mec and Panton-Valentine Leukocidin Gene Amongst the Methicillin-resistant Staphylococcus aureus Strains Isolated From Fowl Meat

Saadati

Mashak

Yarmand

2019

Int J Enteric Pathog

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) is considered to be one of the most important causes of foodborne diseases. Objective: The current examination was performed to examine the distribution of staphylococcal cassette chromosome mec (SCCmec) and Panton-Valentine leukocidin (PVL) gene amongst the MRSA strains isolated from raw fowl meat samples. Materials and Methods: A total of 240 fowl meat samples were collected and cultured. MRSA strains were identified using cefoxitin and oxacillin susceptibility tests. DNA samples extracted from the MRSA strains were subjected to polymerase chain reaction (PCR) for detection of SCCmec and PVL gene. Results: Twenty-two out of 240 (9.16%) raw fowl meat samples were positive for S. aureus strains. Twelve out of 22 S. aureus strains (54.54%) were determined as MRSA strains. The incidence of MRSA strains in raw chicken, turkey, quail, and ostrich meat samples was 66.66%, 50%, 50%, and 33.33%, respectively. The incidence of SCCmec IVa, SCCmec IVd, and SCCmec V was 50%, 8.33% and 41.66%, respectively. The applied method failed to detect SCCmec types I, II, III, IVb, and IVc. The incidence of the PVL gene amongst the MRSA strains was 75%. Conclusion: The presence of SCCmec IV and SCCmec V and PVL gene revealed occurrence of community-associated MRSA (CA-MRSA) in fowl meat samples. Further studies are required to find additional epidemiological aspects of the MRSA strains in fowl meat samples.

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MRSA and multidrug-resistant Staphylococcus aureus in U.S. retail meats, 2010–2011

Cited by 85 publications

References 37 publications

The Genomic Context for the Evolution and Transmission of Community-Associated Staphylococcus aureus ST59 Through the Food Chain

The Genomic Context for the Evolution and Transmission of Community-Associated Staphylococcus aureus ST59 Through the Food Chain

Inhibition of Staphylococcus aureus LC 554891 by Moringa oleifera Seed Extract either Singly or in Combination with Antibiotics

Prevalence of Staphylococcal Cassette Chromosome mec and Panton-Valentine Leukocidin Gene Amongst the Methicillin-resistant Staphylococcus aureus Strains Isolated From Fowl Meat

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