MRP5b/SMRP mRNA is Highly Expressed in Metallothionein- Deficient Mouse Liver

Suzuki, Toshihiro; Agui, Mika; Togawa, Tadayasu; Naganuma, Akira; Nishio, Kazuto; Tanabe, Shinzo

doi:10.1248/jhs.49.524

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Rna Isolation and Rt-pcr Analysis1

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2007

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“…cDNA synthesis and the polymerase chain reaction were carried out using the ReverTra Dash RT-PCR kit according to the manufacturer's protocol ( TOYOBO, Tokyo, JAPAN) as described previously (Suzuki et al, 2003).…”

Section: Rna Isolation and Rt-pcr Analysismentioning

confidence: 99%

Arsenic Accumulation Decreased in Metallothionein Null Cisplatin-Resistant Cell Lines

et al. 2007

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-Metallothionein (MT) is known to play an important role in the resistance of tumor cells to cis-diamminedichloroplatinum (II) (cisplatin). To identify non-MT factors of cisplatin resistance, we characterized cisplatin-resistant cell lines derived from MT-null cells. All of the cisplatin-resistant MTnull cell lines, namely MKCr-1, -3, -4, -12, and -13, showed strong cisplatin resistance and decreased platinum accumulation. Some multidrug resistance proteins (MRPs) have been reported to contribute to cisplatin resistance. However, no significant difference of the MRPs was observed in any of these cell lines. The MKCrs showed cross-resistance to other metals such as arsenite, arsenate, cadmium and antimony. The arsenate and arsenite sensitivities were highly correlated with sensitivity to cisplatin. In addition, the degree of arsenite accumulation was correlated with the degree of cisplatin accumulation. These results suggest that the cisplatin resistance was strongly correlated with the arsenite transport mechanism in these cells.

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Section: Rna Isolation and Rt-pcr Analysismentioning

confidence: 99%