mRNA transcripts of several plant genes are polyadenylated at multiple sitesin vivo

Abstract

We have analyzed the polyadenylation sites for the small subunit of ribulose bisphosphate carboxylase and chlorophyll a/b binding protein genes of Petunia (Mitchell) and the bronze gene of Zea mays. Sequence analysis of multiple cDNA clones revealed that polyadenylation of the transcripts occurred at either 2 or 3 sites for all three groups of genes. In the examples where 3 polyadenylation sites were detected, the middle site was the one predominantly used. Putative polyadenylation signals preceding the poly A tails diverged significantly from the animal consensus sequence AATAAA. In all the genes examined the first A residue in the poly A tail of the cDNA clones corresponded to an A residue in the homologous genomic sequence.

Further analysis of cDNA clones for maize phosphoenolpyruvate carboxylase involved in C4 photosynthesis Nucleotide sequence of entire open reading frame and evidence for polyadenylation of mRNA at multiple sites in vivo

“…Such sequences can also be depicted for the present case as boxed, in fig.3. Thus our data present an additional example to the proposal by Dean et al [16] that the polyadenylation frequently occurs at multiple sites in plant mRNAs.…”

“…For each polyadenylation site, a putative polyadenylation signal can be depicted as in fig.3. The signal sequence for the first site may be AATAAT located 18 nt upstream, since this sequence coincides exactly with the consensus sequence proposed for plants [16]. For the second and third sites the same consensus sequence which is located 17 or 18 nt upstream seems to be used but the ambiguity of the processing of mRNA in vivo might have produced one nucleotide difference in the polyadenylation sites.…”

Nucleotide sequences of cDNA clones for C-hordein polypeptides

“…Analyses of B-hordein cDNA clones have shown that B-hordein messenger RNAs are polyadenylated at two sites (22) suggesting that both signals can be recognized by the poly(A)-adding enzymes. Multiple polyadenylation patterns have also been observed in the mRNAs coding for ribulose bisphosphate carboxylase and chlorophyll a/bbinding proteins of petunia (8).…”

Insect Resistance in Transgenic Chrysanthemum [Dendranthema * grandiflorum (Ramat.) Kitamura] by the Introduction of a Modified .DELTA.-endotoxin Gene of Bacillus thuringiensis

“…On the other hand, in the case of post-transcriptional silencing, mRNA does not accumulate even though transcription occurs, and it The five first instar larvae of H. armigera were allowed to feed on leaves of transformed and non-transformed plants. C: non-transformed plant 'Shuho-no-chikara' 1-2: wild type cry1Ab-transformed lines 3-8: mcbt-transformed lines Lines; indicate the name of the transformed lines, Cry1Ab ICP; indicates the Cry1Ab expression level (ng/50 µg total protein) TP; total protein has been suggested that this phenomenon is mainly due to the overproduction and specific degradation of transgene mRNA (Dean et al 1986, Shaw and Kamen 1986, Goodall and Filipowicz 1989. In the present experiment, some of the transgenic chrysanthemums which did not show a weak or an absence of insect resistance harbored 3 copies of the mcbt gene.…”

“…In this paper, we modified the coding sequence of the cry1Ab gene by removing ATTTA sequences (Dean et al 1986, Shaw and Kamen 1986, Goodall and Filipowicz 1989 and by using the preferred codons of Compositae family to increase the cry1Ab expression in chrysanthemum. The modified cry1Ab gene was highly expressed in the transgenic chrysanthemum, and the resultant transformants were significantly more resistant to the lepidopteran pest H. armigera.…”