mRNA localization in the<i>Drosophila</i>germline

Weil, Timothy T

doi:10.4161/rna.36097

Cited by 29 publications

(23 citation statements)

References 119 publications

(137 reference statements)

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“…Although numerous mRNAs are specifically localized within the Drosophila egg chamber (Jambor et al 2015), the localization pathways of gurken (grk), bicoid (bcd), and oskar (osk) mRNAs have been most extensively characterized (Gonsalvez and Long 2012;Weil 2014). Lysates expressing GFP or Egl-GFP were subjected to immunoprecipitation using GFP antibody beads.…”

Section: Resultsmentioning

confidence: 99%

Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber

et al. 2016

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The Drosophila egg chamber provides a useful model for examining mechanisms by which cell fates are specified and maintained in the context of a complex tissue. The egg chamber is also an excellent model for understanding the mechanism by which cytoskeletal filaments are organized and the critical interplay between cytoskeletal organization, polarity establishment, and cell fate specification. Previous work has shown that Egalitarian (Egl) is required for specification and maintenance of oocyte fate. Mutants in egl either completely fail to specify an oocyte, or if specified, the oocyte eventually reverts back to nurse cell fate. Due to this very early role for Egl in egg chamber maturation, it is unclear whether later stages of egg chamber development also require Egl function. In this report, we have depleted Egl at specific stages of egg chamber development. We demonstrate that in early-stage egg chambers, Egl has an additional role in organization of oocyte microtubules. In the absence of Egl function, oocyte microtubules completely fail to reorganize. As such, the localization of microtubule motors and their cargo is disrupted. In addition, Egl also appears to function in regulating the translation of critical polarity determining messenger RNAs (mRNAs). Finally, we demonstrate that in midstage egg chambers, Egl does not appear to be required for microtubule organization, but rather for the correct spatial localization of oskar, bicoid, and gurken mRNAs.

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Section: Resultsmentioning

confidence: 99%

Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber

et al. 2016

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“…Other recent studies are also beginning to imply similar levels of localization in other organisms (Taliaferro et al 2014;Weil 2014;Jambor et al 2015;Liao et al 2015). However, functional studies that validate the relative importance and underlying mechanisms behind the majority of these localization events are lagging behind.…”

Section: Subcellular Localization In Other Organismsmentioning

confidence: 99%

Diverse and pervasive subcellular distributions for both coding and long noncoding RNAs

et al. 2016

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In a previous analysis of 2300 mRNAs via whole-mount fluorescent in situ hybridization in cellularizing Drosophila embryos, we found that 70% of the transcripts exhibited some form of subcellular localization. To see whether this prevalence is unique to early Drosophila embryos, we examined ∼8000 transcripts over the full course of embryogenesis and ∼800 transcripts in late third instar larval tissues. The numbers and varieties of new subcellular localization patterns are both striking and revealing. In the much larger cells of the third instar larva, virtually all transcripts observed showed subcellular localization in at least one tissue. We also examined the prevalence and variety of localization mechanisms for >100 long noncoding RNAs. All of these were also found to be expressed and subcellularly localized. Thus, subcellular RNA localization appears to be the norm rather than the exception for both coding and noncoding RNAs. These results, which have been annotated and made available on a recompiled database, provide a rich and unique resource for functional gene analyses, some examples of which are provided.

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“…Posttranscriptional mechanisms play pivotal roles in controlling these events by regulating mRNA localization and translation during oogenesis (4)(5)(6). For example, Drosophila hnRNP A1 homolog Hrb98DE/Hrp38 controls E-cadherin translation by binding to the 5= untranslated region (UTR) of E-cadherin mRNA for germ line stem cell self-renewal (7).…”

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confidence: 99%

Poly(ADP-Ribosyl)ation of hnRNP A1 Protein Controls Translational Repression in Drosophila

Tulin

2016

Molecular and Cellular Biology

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Poly(ADP-ribosyl)ation of heterogeneous nuclear ribonucleoproteins (hnRNPs) regulates the posttranscriptional fate of RNA during development. Drosophila hnRNP A1, Hrp38, is required for germ line stem cell maintenance and oocyte localization. The mRNA targets regulated by Hrp38 are mostly unknown. We identified 428 Hrp38-associated gene transcripts in the fly ovary, including mRNA of the translational repressor Nanos. We found that Hrp38 binds to the 3= untranslated region (UTR) of Nanos mRNA, which contains a translation control element. We have demonstrated that translation of the luciferase reporter bearing the Nanos 3= UTR is enhanced by dsRNA-mediated Hrp38 knockdown as well as by mutating potential Hrp38-binding sites. Our data show that poly(ADP-ribosyl)ation inhibits Hrp38 binding to the Nanos 3= UTR, increasing the translation in vivo and in vitro. hrp38 and Parg null mutants showed an increased ectopic Nanos translation early in the embryo. We conclude that Hrp38 represses Nanos translation, whereas its poly(ADP-ribosyl)ation relieves the repression effect, allowing restricted Nanos expression in the posterior germ plasm during oogenesis and early embryogenesis.

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mRNA localization in theDrosophilagermline

Cited by 29 publications

References 119 publications

Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber

Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber

Diverse and pervasive subcellular distributions for both coding and long noncoding RNAs

Poly(ADP-Ribosyl)ation of hnRNP A1 Protein Controls Translational Repression in Drosophila

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