mRNA and protein expression of the angiogenesis-related genes<i>EDIL3, AMOT</i>and<i>ECM1</i>in mesenchymal stem cells in psoriatic dermis

Niu, Xuping; Chang, Wenjuan; Liu, R; Hou, Ruixia; Li, J; Wang, C; Li, X; Zhang, K

doi:10.1111/ced.12783

Cited by 27 publications

(33 citation statements)

References 25 publications

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“…An eQTL at the same SNP is reported across many tissues in GTEx, and affects a number of genes in the region, including ECM1, RP11-54A4.2, MRPS21, HORMAD1, GOLPH3L, and TARS2, and in many cases rs6693567 is the lead SNP for those associations. It is not clear which of these genes is most relevant, although ECM1 is reported to have roles in angiogenesis (Han et al 2001) and has been associated with psoriasis (Niu et al 2016) and inflammatory bowel disease (Fisher et al 2008). The most highly expressed of these genes in IPSDSNs is the mitochondrial ribosomal subunit MRPS21 consistent with reports of a link between mitochondrial dysfunction and migraine (Yorns and Hardison 2013).…”

Section: Sensory Neuron Eqtls and Sqtls Overlap With Complex Trait Locimentioning

confidence: 61%

Molecular and functional variation in iPSC-derived sensory neurons

Schwartzentruber

Foskolou

Kilpinen

et al. 2017

Preprint

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Induced pluripotent stem cells (iPSCs), and cells derived from them, have become key tools to model biological processes and disease mechanisms, particularly in cell types such as neurons that are difficult to access from living donors. Here, we present the first map of regulatory variants in an iPSC-derived cell type. To investigate genetic contributions to human sensory function, we performed 123 differentiations of iPSCs from 103 unique donors to a sensory neuronal fate, and measured gene expression, chromatin accessibility, and neuronal excitability. Compared with primary dorsal root ganglion, where sensory nerves collect near the spinal cord, gene expression was more variable across iPSC-derived neuronal cultures, particularly in genes related to differentiation and nervous system development. Single cell RNA-sequencing revealed that although the majority of cells are neuronal and express the expected marker genes, a substantial fraction have a fibroblast-like expression profile. By applying an allele-specific method we identify 3,778 quantitative trait loci influencing gene expression, 6,318 for chromatin accessibility, and 2,097 for RNA splicing at FDR 10%. A number of these overlap with common disease associations, and suggest candidate causal variants and target genes. These include known causal variants at SNCA for Parkinson's disease and TNFRSF1A for multiple sclerosis, as well as new candidates for migraine, Parkinson's disease, and schizophrenia.

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Section: Sensory Neuron Eqtls and Sqtls Overlap With Complex Trait Locimentioning

confidence: 61%

Molecular and functional variation in iPSC-derived sensory neurons

Schwartzentruber

Foskolou

Kilpinen

et al. 2017

Preprint

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show abstract

“…In the same year, Niu et al 97 studied the molecular regulation of angiogenesis by DMSCs investigating the mRNA and protein expression of AMOT, EDIL3, and ECM1 in DMSCs isolated from psoriatic skin, with the aim of a better identification of the molecular pathway of angiogenesis in psoriatic skin.…”

Section: Discussionmentioning

confidence: 99%

“…The authors of that study 97 cultured DMSCs, identified by morphology, immunophenotype, and multipotential differentiation, derived from 12 patients with psoriasis and 14 healthy controls. They also evaluated the mRNA and protein expressions of ECM1, EDIL3, and AMOT in the DMSCs using real-time reverse transcription–PCR and Western blotting.…”

Section: Discussionmentioning

confidence: 99%

“…Protein analysis showed significantly higher concentrations of ECM1, EDIL3, and AMOT in the psoriasis group than in the HC group. 97 …”

Section: Discussionmentioning

confidence: 99%

“…From this review, it emerges that MSCs are involved in the immunoregulation and angiogenesis of psoriasis and that they are probably related to some modification occurring in psoriasis (capillary proliferation, angiectasias, or inflammatory infiltration). 93 , 94 , 97 …”

Section: Discussionmentioning

confidence: 99%

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Role of mesenchymal stem cells in the pathogenesis of psoriasis: current perspectives

Campanati

Consales

Orciani

et al. 2017

PTT

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Dermal mesenchymal stem cells promoted adhesion and migration of endothelial cells by integrin in psoriasis

Han

Niu

Hou

et al. 2020

Cell Biology International

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The unusual dilatation of dermal capillaries and angiogenesis played important roles in psoriasis. Some genes and proteins of dermal mesenchymal stem cells (DMSCs) from psoriasis are abnormal and related to the function of endothelial cells (ECs). The present study was aimed to evaluate whether psoriatic DMSCs could affect adhesion and migration of ECs through neovascularization‐related integrins in psoriasis. Human DMSCs, collected from psoriasis lesions and healthy skin, respectively, were cocultured with human umbilical vein endothelial cells (HUVECs). The expression levels of three integrins, that is, αvβ3, αvβ5, and α5β1 in HUVECs were tested by quantitative real‐time polymerase chain reaction and Western blot analysis. The adhesion and migration of HUVECs were detected by adhesion assay and migration assay. The results showed that in psoriasis group, the expression of αVβ3 and α5β1 of HUVECs markedly increased 2.50‐ and 3.71‐fold in messenger RNA levels, and significantly increased 1.63‐ and 1.92‐fold in protein levels, comparing to healthy control group (all p < .05). But β5 was not significantly different between the two groups (p > .05). In addition, compared with control, psoriatic DMSCs promoted HUVECs adhesion by 1.62‐fold and migration by 2.91‐fold (all p < .05). In conclusion, psoriatic DMSCs impact HUVECs adhesion and migration by upregulating the expression of integrins αVβ3 and α5β1.

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mRNA and protein expression of the angiogenesis-related genesEDIL3, AMOTandECM1in mesenchymal stem cells in psoriatic dermis

Abstract: Our data demonstrate for the first time that expression of EDIL3, AMOT and ECM1 is altered in DMSCs in psoriasis, suggesting that EDIL3, AMOT and ECM1 are involved in the excessive angiogenesis and vasodilation observed in psoriasis.

Cited by 27 publications

References 25 publications

Molecular and functional variation in iPSC-derived sensory neurons

Molecular and functional variation in iPSC-derived sensory neurons

Role of mesenchymal stem cells in the pathogenesis of psoriasis: current perspectives

Dermal mesenchymal stem cells promoted adhesion and migration of endothelial cells by integrin in psoriasis

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