MRI Virtual Biopsies: Analysis of an Explanted Endovascular Device and Perspectives for the Future

Guidoin, Robert; Zhang, Ze; Douville, Yvan; Bonny, Jean‐Marie; Renou, Jean-Pierre; Basle, Michel‐Félix; Zarins, C K; Legrand, A. P.; Guzman, Randolph

doi:10.1080/10731190600581825

Cited by 3 publications

(2 citation statements)

References 58 publications

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“…The aorta was removed en bloc with the stent graft in place for explant analysis. A more detailed clinical data is reported elsewhere [40].…”

Section: Clinical Reportmentioning

confidence: 99%

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Polymethylmethacrylate (PMMA) as an Embedding Medium Preserving Tissues and Foreign Materials Encroaching in Endovascular Devices

Guidoin¹,

Zhang²,

Douville³

et al. 2006

Artificial Cells, Blood Substitutes, and Biotechnology

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Problems of displacement, poor healing, degradation of the polymers and corrosion of the metallic frame in endovascular devices still require in-depth investigations. As the tissues and the foreign materials are in close contact, it is of paramount importance to efficiently investigate the interfaces between them. 349 Inclusion in polymethymethacrylate (PMMA) permits us to obtain thin slides and preserve the capacity to perform the appropriate stainings.An AneuRx prosthesis was harvested in bloc with the surrounding tissues at the autopsy of a patient 25 months post deployment in a 5.7 cm diameter AAA and sectioned in the direction of the blood flow in two halves. A cross-section of the encapsulated distal segment together with the surrounding aneuryshmal sac was embedded in polymethylmethacrylate (PMMA). Further to complete polymerization, slices of the specimen were cut on a precision banding saw under coolant. They were affixed onto methacrylate slides with a UV cured adhesive. Binding and polishing were done on a numeric grinder and slices 25 to 30 mm in thickness were stained with toluidine blue prior to observation in light microscopy. Additional slices were prepared for scanning electron microscopy and X-ray energy dispersive spectrometry for determination of the elemental composition of the Nitinol stent.The aortic wall did not demonstrate complete integrity along with its circumference. Some areas of rupture were noted. The content of the sac was heavily shrunk and was mostly acellular. The walls of the device were very well encapsulated. The PMMA embedding permitted the polyester wall, the Nitinol wire and the collagen to keep in close contact. Scanning electron microscopy involved backscattered electrons and confirmed the corrosion the Nitinol wire at the boundary with living tissues.Based upon the results obtained, we believe that PMMA embedding is the most appropriate method to process endovascular devices for histological and material investigation. Needless to say, that paraffin embedding would have not been feasible for such a big size specimen involving different materials.

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“…The aorta was removed en bloc with the stent graft in place for explant analysis. A more detailed clinical data is reported elsewhere [40].…”

Section: Clinical Reportmentioning

confidence: 99%

“…Follow-up of the devices to validate their 3Bs, i.e. biocompatibility, biofunctionality and bioresilience, will necessitate not only the current techniques but also the development of virtual biopsies [40].…”

mentioning

confidence: 99%

Polymethylmethacrylate (PMMA) as an Embedding Medium Preserving Tissues and Foreign Materials Encroaching in Endovascular Devices

Guidoin¹,

Zhang²,

Douville³

et al. 2006

Artificial Cells, Blood Substitutes, and Biotechnology

Self Cite

View full text Add to dashboard Cite

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Pathology of graft and stent-graft infections: Lessons learned from examination of explant materials

Lejay¹,

Monnot²,

Georg³

et al. 2017

Seminars in Vascular Surgery

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Newly Developed Hybrid Suture without Lubricant: Noninvasive In Vivo Assessment of Biocompatibility with Multiparametric MR Imaging

Traoré

Guidoin

Marois

et al. 2007

Journal of Investigative Surgery

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Magnetic resonance imaging (MRI) and magnetic resonance (MR) relaxometry were used to assess noninvasively the tissue response of a new uncoated hybrid braided suture made from a combination of ultra-high-molecular-weight polyethylene (UHMWPE) and polyester (polyethylene terephthalate) (PET) yarns in comparison to a silicone impregnated braided 100% polyester (PET) control suture (Ticron). Both biomaterials were monitored for a period of 30 days following implantation in both incised and nonincised paravertebral rabbit muscles. In all cases, MR images and relaxometry demonstrated that the hybrid suture elicited either a milder or a similar tissue and cellular response compared to the control suture. These findings were confirmed by conventional histological analysis of the surrounding tissues. They also demonstrated that the hybrid suture promoted faster healing in terms of collagen infiltration between the yarns and individual filaments. This milder inflammatory reaction and improved biocompatibility represent a real advantage in the healing performance of sutures for cardiac and vascular surgery, and support the need for continued research and development of hybrid structures. This study also demonstrated the ability of MRI techniques to noninvasively evaluate the biocompatibility of biomaterials. By extending the capacity of MR diagnostic tools from patients to experimental animals, it is now possible to validate the healing performance of foreign materials with statistical reliability and fewer animals.

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MRI Virtual Biopsies: Analysis of an Explanted Endovascular Device and Perspectives for the Future

Cited by 3 publications

References 58 publications

Polymethylmethacrylate (PMMA) as an Embedding Medium Preserving Tissues and Foreign Materials Encroaching in Endovascular Devices

Polymethylmethacrylate (PMMA) as an Embedding Medium Preserving Tissues and Foreign Materials Encroaching in Endovascular Devices

Pathology of graft and stent-graft infections: Lessons learned from examination of explant materials

Newly Developed Hybrid Suture without Lubricant: Noninvasive In Vivo Assessment of Biocompatibility with Multiparametric MR Imaging

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