MRI of lung parenchyma in rats and mice using a gradient‐echo sequence

Beckmann, Nicolau; Tigani, Bruno; Mazzoni, Lazzaro; Fozard, John R.

doi:10.1002/nbm.706

Cited by 60 publications

(65 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Histological examinations of rats (1,26) have shown an elevated presence of inflammatory cells and cytokines at four days and a further increment at six days after BLM-treatment, as well as protein-rich granular-textured edema in the alveolar spaces. Consistent with this, increased levels of protein in BAL fluid of BLM-treated rats one week after exposure have also been reported (31).…”

Section: Discussionmentioning

confidence: 99%

“…For detecting changes at the level of lung parenchyma, short TE gradient-echo images were acquired with the following parameters (26): rise time of the gradients ϭ 60 sec; matrix size ϭ 36 ϫ 128; field-ofview ϭ 6 ϫ 6 cm 2 for transverse slices or 6 ϫ 10 cm 2 for coronal ones; slice thickness ϭ 2 mm; TR ϭ 1.95 msec; TE ϭ 555 s; and averages ϭ 80. The acquisition of an individual image took 250 msec.…”

Section: Mri Acquisition Protocolsmentioning

confidence: 99%

“…A single slice image was obtained by computing the 2DFT of the averaged signal from 80 individual image acquisitions and interpolating the data set to 256 ϫ 256 pixels. The choice for averaging 80 individual images was based on previous experiments (19,26,27) in which cardiac and respiratory movement artifacts were successfully suppressed in this way. There was an interval of 500 msec between individual image acquisitions.…”

Section: Mri Acquisition Protocolsmentioning

confidence: 99%

See 2 more Smart Citations

Bleomycin‐induced lung injury assessed noninvasively and in spontaneously breathing rats by proton MRI

Karmouty‐Quintana

Cannet

Zurbruegg

et al. 2007

Magnetic Resonance Imaging

Self Cite

View full text Add to dashboard Cite

Purpose:To apply proton magnetic resonance imaging (MRI) techniques to assess noninvasively and in spontaneously breathing rats, structural changes following a single intratracheal administration of bleomycin (BLM). Materials and Methods:Rats were scanned by MRI prior to BLM or vehicle administration and at six hours, 24 hours, week 1, and at weeks 2, 3, 6, and 8 after treatment. Bronchoalveolar lavage (BAL) fluid and histological analyses were performed at 24 hours, and at weeks 1 and 8 (histology only).Results: Prominent MRI fluid signals were detected in the lungs of BLM-treated rats one week after challenge. These signals correlated with increased inflammatory parameters in BAL fluid and with marked perivascular and parenchymal infiltration with inflammatory cells in histological slices. At week 2 the MRI signals due to edema resolved, but nevertheless an increase in MRI signal intensity from the lung parenchyma was apparent. In some areas of the right lung the MRI signal intensity in the parenchyma decreased between weeks 2 and 8. These observations were in line with histology demonstrating collagen deposition and atelectasis (hallmarks of fibrosis) at week 1 and a partial recovery of the lung parenchyma at week 8. Conclusion:The data demonstrate the ability of proton MRI to detect BLM-induced lung fibrosis as well as the acute inflammatory response caused by the agent.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Mri Acquisition Protocolsmentioning

confidence: 99%

Section: Mri Acquisition Protocolsmentioning

confidence: 99%

See 1 more Smart Citation

Bleomycin‐induced lung injury assessed noninvasively and in spontaneously breathing rats by proton MRI

Karmouty‐Quintana

Cannet

Zurbruegg

et al. 2007

Magnetic Resonance Imaging

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sharp images from the mouse chest could be obtained by image averaging, despite the fact that neither respiratory nor cardiac gating was applied (242,245). Animals respired spontaneously during data collection.…”

Section: Respiratory Diseasesmentioning

confidence: 99%

In Vivo mouse imaging and spectroscopy in drug discovery

et al. 2007

Self Cite

View full text Add to dashboard Cite

Imaging modalities such as micro-computed tomography (micro-CT), micro-positron emission tomography (micro-PET), high-resolution MRI, optical imaging, and high-resolution ultrasound have become invaluable tools in preclinical pharmaceutical research. They can be used to non-invasively investigate, in vivo, rodent biology and metabolism, disease models, and pharmacokinetics and pharmacodynamics of drugs. The advantages and limitations of each approach usually determine its application, and therefore a small-rodent imaging laboratory in a pharmaceutical environment should ideally provide access to several techniques. In this paper we aim to illustrate how these techniques may be used to obtain meaningful information for the phenotyping of transgenic mice and for the analysis of compounds in murine models of disease.

show abstract

“…TOXICOLOGIC PATHOLOGY to study pulmonary edema resulting from allergen challenge in rats (Beckmann et al, 2001). …”

Section: Driehuys and Hedlundmentioning

confidence: 99%

Imaging Techniques for Small Animal Models of Pulmonary Disease: MR Microscopy

Driehuys

Hedlund

2007

Toxicol Pathol

View full text Add to dashboard Cite

In vivo magnetic resonance microscopy (MRM) of the small animal lung has become a valuable research tool, especially for preclinical studies. MRM offers a noninvasive and nondestructive tool for imaging small animals longitudinally and at high spatial resolution. We summarize some of the technical and biologic problems and solutions associated with imaging the small animal lung and describe several important pulmonary disease applications. A major advantage of MR is direct imaging of the gas spaces of the lung using breathable gases such as helium and xenon. When polarized, these gases become rich MR signal sources. In animals breathing hyperpolarized helium, the dynamics of gas distribution can be followed and airway constrictions and obstructions can be detected. Diffusion coefficients of helium can be calculated from diffusion-sensitive images, which can reveal micro-structural changes in the lungs associated with pathologies such as emphysema and fibrosis. Unlike helium, xenon in the lung is absorbed by blood and exhibits different frequencies in gas, tissue, or erythrocytes. Thus, with MR imaging, the movement of xenon gas can be tracked through pulmonary compartments to detect defects of gas transfer. MRM has become a valuable tool for studying morphologic and functional changes in small animal models of lung diseases.

show abstract

MRI of lung parenchyma in rats and mice using a gradient‐echo sequence

Cited by 60 publications

References 33 publications

Bleomycin‐induced lung injury assessed noninvasively and in spontaneously breathing rats by proton MRI

Bleomycin‐induced lung injury assessed noninvasively and in spontaneously breathing rats by proton MRI

In Vivo mouse imaging and spectroscopy in drug discovery

Imaging Techniques for Small Animal Models of Pulmonary Disease: MR Microscopy

Contact Info

Product

Resources

About