MRI findings of obstructive azoospermia: lesions in and out of pelvic cavity

Wang, Huanjun; Yang, Peng; Fu, Wenhao; Hu, Xiaoxiao; Li, Chang; Guan, Jian

doi:10.1007/s00261-019-02375-8

Cited by 3 publications

(2 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the treatment, 37%-70% of male's partners still cannot get pregnant, which troubles many OA patients. 46 Multiple studies have shown that OA is characterized by normal testis volume, normal endocrine function, and normal spermatogenesis, 4,47 concordance with our histological examination. This opens the question of why the OA patients have normal spermatogenesis in the testis but a low fertility after treatment compared to normal adult males.…”

Section: Discussionsupporting

confidence: 84%

Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia

et al. 2023

View full text Add to dashboard Cite

Obstructive azoospermia (OA) accounts for approximately 40% of males who suffer from azoospermia of male infertility. Currently, available treatment for OA consists of reproductive tract surgical reconstruction and sperm retrieval from the testis. However, both treatments result in low fertility compared to normal pregnancy, and the main reason remains largely unknown. Previous studies have shown that the quality of sperm retrieved from OA patients is poor compared with normal adult males but without an in‐depth study. Herein, we generated a mouse OA model with vasectomy to evaluate sperm quality systematically. Our results showed that the testis had normal spermatogenesis but increased apoptotic activity in both OA patients and mice. More importantly, epididymal morphology was abnormal, with swollen epididymal tubules and vacuole‐like principal cells. Especially, sperm retrieved from the epididymis of OA mice showed poor motility and low fertilization ability in vitro. Using mass spectrometry in epididymal fluid, we found differences in the expression of key proteins for sperm maturation, such as Angiotensinogen (AGT), rhophilin‐associated tail protein 1 (ROPN1), NPC intracellular cholesterol transporter 2 (NPC2), and prominin 1 (PROM1). Furthermore, our results demonstrated that AGT, secreted by epididymal principal cells, could regulate sperm motility by managing PKCα expression to modify sperm phosphorylation. In conclusion, our data evaluate sperm quality systematically in OA mice and contribute to the understanding between the sperm and epididymis, which may provide novel insight into treating male infertility.

show abstract

Section: Discussionsupporting

confidence: 84%

Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Obstructive azoospermia was clinically diagnosed by semen tests (no sperm in ejaculates) and the existence of excurrent duct obstruction 93 . According to traditional opinion, OA patients were with rather conserved spermatogenesis 94 . Therefore, in traditional studies, due to the difficulty in obtaining completely healthy and fertile male testicular samples 95 , 96 , researchers would like to choose OA samples with confirmed normal histopathologic features as the controls 97 - 100 .…”

Section: Resultsmentioning

confidence: 99%

Application of single-cell RNA sequencing on human testicular samples: a comprehensive review

Fan¹,

Ping²,

Ma³

et al. 2023

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Differentiation between nonobstructive azoospermia and obstructive azoospermia: then and now

Hubbard,

Rambhatla,

Colpi

2024

Asian Journal of Andrology

View full text Add to dashboard Cite

Male infertility has seen an increase in prevalence with cases of azoospermia estimated to affect 10%–15% of infertile men. Confirmation of azoospermia subsequently necessitates an early causal differentiation between obstructive azoospermia (OA) and nonobstructive azoospermia (NOA). Although less common when compared to NOA, OA can represent upward 20%–40% of cases of azoospermia. While there are a multitude of etiologies responsible for causing NOA and OA, correctly distinguishing between the two types of azoospermia has profound implications in managing the infertile male. This review represents an amalgamation of the current guidelines and literature which will supply the reproductive physician with a diagnostic armamentarium to properly distinguish between NOA and OA, therefore providing the best possible care to the infertile couple.

show abstract

MRI findings of obstructive azoospermia: lesions in and out of pelvic cavity

Cited by 3 publications

References 43 publications

Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia

Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia

Application of single-cell RNA sequencing on human testicular samples: a comprehensive review

Differentiation between nonobstructive azoospermia and obstructive azoospermia: then and now

Contact Info

Product

Resources

About