Texture analysis is a technique used for the quantification of image texture. It has been successfully used in many fields, and in the past years it has been applied in magnetic resonance imaging (MRI) as a computer-aided diagnostic tool. Quantification of the intrinsic heterogeneity of different tissues and lesions is necessary as they are usually imperceptible to the human eye. In the present chapter, we describe texture analysis as a process consisting of six steps: MRI acquisition, region of interest (ROI) definition, ROI preprocessing, feature extraction, feature selection, and classification. There is a great variety of methods and techniques to be chosen at each step and all of them can somehow affect the outcome of the texture analysis application. We reviewed the literature regarding texture analysis in clinical MRI focusing on the important considerations to be taken at each step of the process in order to obtain maximum benefits and to avoid misleading results.