MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen.

Spickett, Gavin P; Brandon, Malcolm R.; Mason, Donald W.; Williams, Alan F.; Woollett, Gillian R.

doi:10.1084/jem.158.3.795

Cited by 215 publications

(109 citation statements)

References 33 publications

(42 reference statements)

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“…HIS25 (CD45RC; LCA isotype present on all Tcyt/suppr., part of Th and all B cells) [46] and HIS51 (Thy1.1, within the TCR + cell population specific for RTE) [47] were produced by the Department of Histology, University of Groningen, The Netherlands. W3/25 (CD4) [48], OX6 (RT1B; MHC class II) [49], OX22 (CD45RC) [50] and OX35 (CD4) [14] were obtained from the European Collection of Animal Cell Culture (Salisbury, UK). PEconjugated OX8 (CD8 , Tcyt/suppr.…”

Section: Antibodiesmentioning

confidence: 99%

Susceptibility to clinically manifest cyclosporine A (CsA)-induced autoimmune disease is associated with interferon-gamma (IFN-γ)-producing CD45RC+RT6− T helper cells

Beijleveld

Groen

Broeren

et al. 1996

Clinical and Experimental Immunology

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SUMMARYLethally irradiated Lewis (LEW) rats reconstituted with syngeneic bone marrow and given CsA for a 4-week period, develop, upon withdrawal of CsA, a graft-versus-host-like disease, so-called CsA-induced autoimmunity (CsA-AI). This T cell-mediated autoimmune disease is thymus-dependent; it is generally held that this disease is a consequence of aberrant T cell recovery brought about by CsA. In this study we determined mononuclear cell subsets phenotypically by tri-colour flow cytometry. A strong decrease in recent thymic emigrants (Thy1.1 + , TCR ® ¯ + ) was observed as a consequence of CsA treatment, eventually resulting in decreased absolute peripheral T cell numbers. In these rats no altered CD4:CD8 T cell ratio was observed before onset of CsA-AI; CD4 + and CD8 + cells consisted predominantly of monocytes (CD4 dim+ , TCR ® ¯ -) and natural killer cells (CD8 + , TCR ® ¯ -), respectively. LEW rats, xirradiated, syngeneic bone marrow-reconstituted and treated with CsA, showed a marked and persistent, relative expansion of mature CD45RC + , RT6 -Th cells. In contrast, Brown-Norway rats treated in a similar fashion, or LEW rats subjected to either CsA treatment or x-irradiation, did not show a comparable expansion of mature CD45RC + , RT6 -Th cells, nor did these animals develop CsA-AI. The CD45RC + , RT6 -Th cells produced IL-2, and moreover constituted the only Th subset producing IFN-°u pon stimulation, and therefore were considered as Th1-like effector cells. These results are consistent with the view that a persistent preponderance of Th1 cells and not the mere presence of autoreactive cells determines whether or not clinically manifest CsA-AI will occur.

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Section: Antibodiesmentioning

confidence: 99%

Susceptibility to clinically manifest cyclosporine A (CsA)-induced autoimmune disease is associated with interferon-gamma (IFN-γ)-producing CD45RC+RT6− T helper cells

Beijleveld

Groen

Broeren

et al. 1996

Clinical and Experimental Immunology

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“…The following monoclonal antibodies were obtained from Serotec and by kind donation from Dr J. Sedgwick: R73 (αβ T cell receptor) (Hünig et al, 1989), OX34 (CD2 on T cells and natural killer (NK) cells) , OX39 (IL-2R) , OX22 (CD45RC, the high molecular mass form of the leukocyte common antigen on B cells, macrophages and some T cells) (Spickett et al, 1983;Woollett et al, 1985), OX8 (CD8 on MHC class I restricted T cells and some NK cells) (Brideau et al, 1980), W3/25 (CD4) (White et al, 1978), OX33 (B cells) (Woollett et al, 1985), OX12 (kappa light chains) (Hunt and Fowler, 1981;Barclay, 1981a), OX6 (MHC class II) Barclay, 1981b); OX42 (CD11b) (Robinson et al, 1986) and OX1 (leukocyte common antigen on all cells of haemopoietic origin) (Sunderland et al, 1979).…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

Inflammatory cells, microglia and MHC class II antigen-positive cells in the spinal cord of Lewis rats with acute and chronic relapsing experimental autoimmune encephalomyelitis

McCombe

Jersey

Pender

1994

Journal of Neuroimmunology

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Chronic relapsing experimental autoimmune encephalomyelitis (CR-EAE) was induced in Lewis rats by inoculation with guinea pig spinal cord and adjuvants and treatment with low dose cyclosporin A (CsA). Acute EAE was induced by the same method without CsA treatment. Immunocytochemistry and flow cytometry were used to assess inflammatory cells and MHC class II (Ia) antigen expression in the central nervous system of these rats. The inflammatory infiltrate was composed mainly of CD4 + T cells and macrophages, and αβ T cells constituted about 65% of the CD2 + T cells. After recovery from acute EAE and during the first remission of CR-EAE, the number of T cells was significantly less than in the preceding episodes. The number of T cells was higher in the second episode of CR-EAE than in the first remission. Throughout the course of CR-EAE, the majority of the CD2 + T cells were CD45RC −. The ratio of IL-2R + cells to CD2 + cells ranged from 10.5 to 24.0%. The ratio of CD4 + T cells to B cells was lower in the later episodes of CR-EAE than in the first episode. Ia antigen was expressed on infiltrating round cells at all stages of CR-EAE and on microglial cells (identified by dendritic morphology) with increasing intensity throughout the course of CR-EAE. With flow cytometry, the number of Ia + cells obtained from the spinal cord rose throughout the course of CR-EAE. The number of FSC low OX1 low cells, which we consider represent microglia, also increased during the course of CR-EAE.

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“…LCA occurs in several molecular weight forms. Only the low molecular weight forms are expressed on CFU-S. B cells and a subset of T-cells that mediate graft versus host disease (20). The ability to label CFU-S with more than one fluorochrome-conjugated monoclonal antibody has allowed us to purify CFU-S from normal, rather than cortisonetreated, rats by histogram pattern recognition and dualparameter immunofluorescence sorting (16).…”

mentioning

confidence: 99%

Purification and analysis of rat hematopoietic stem cells by flow cytometry

McCarthy¹,

Hale²,

Fehnel³

1987

Cytometry

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The monoclonal antibody OX7 recognizes an epitope expressed on the Thy‐1 glycoprotein, OX22 recognizes the high molecular weight forms(s) on leukocyte common antigen, and W3/13 recognized determinants found on certain sialoglycoproteins. Recently, the rat colony‐forming unit spleen (CFU‐S) was characterized as being OX7 upper 20% positive (OX7u20%), OX22 negative (OX22−), and W3/13 weakly positive (W3/13+). In the present study these observations have been extended to include the hematopoietic stem cell (HSC). Rat marow cells were incubated with allophycocyanine‐OX7 Fab' (APC‐OX7 Fab') and phycoerythrin (B‐OX22) Fab' (PhyB‐OX22 Fab'). The cells were sorted with a FACS‐ii instrument by using a Krypton laser tuned to the 530 nm spectral line for phycobiliprotein excitation. It was found that marrow cells capable of protecting lethally irradiated Lewis rats (9.5 Gy total body radiation, 0.4 Gy/min Co60) had the phenotype OX7u20%, OX22−. The percentage of cells in the marrow with this phenotype was found to be 0.34 ± 0.01 (mean ± S.E.). Three thousand of these cells were required to rescue 50% of lethally irradiated recipients (30‐d survival), while the number of unsorted bone marrow cells required was 1.05 × 106. Thus, a 350‐fold purification of the HSC was realized. Although CFU‐S copurified with HSC, purification of only 105‐fold was obtained. This might indicate that purified HSC have a reduced capacity to generate splenic hematopoietic colonies. The OX7u20%, OX22−‐enriched HSC population could be further divided into W3/13 dimand W3/13+ subpopulations by three‐parameter immunofluorescence analysis with the use of a new optical bench arrangement.

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MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen.

Cited by 215 publications

References 33 publications

Susceptibility to clinically manifest cyclosporine A (CsA)-induced autoimmune disease is associated with interferon-gamma (IFN-γ)-producing CD45RC+RT6− T helper cells

Susceptibility to clinically manifest cyclosporine A (CsA)-induced autoimmune disease is associated with interferon-gamma (IFN-γ)-producing CD45RC+RT6− T helper cells

Inflammatory cells, microglia and MHC class II antigen-positive cells in the spinal cord of Lewis rats with acute and chronic relapsing experimental autoimmune encephalomyelitis

Purification and analysis of rat hematopoietic stem cells by flow cytometry

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