MR T1ρ as an imaging biomarker for monitoring liver injury progression and regression: an experimental study in rats with carbon tetrachloride intoxication

Zhao, Feng; Wáng, Yì Xiáng J.; Yuan, Jing; Deng, Min; Wong, Hai Ming; Chu, Eagle Sh; Go, Minnie Y.Y.; Teng, Gao‐Jun; Ahuja, Anil T.; Yu, Jun

doi:10.1007/s00330-012-2419-0

Cited by 53 publications

(64 citation statements)

References 46 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…How these processes contribute to the elevated T1rho measurement deserve careful investigation, which can be answered partially by careful animal model studies. In an imperfect rat study reported by us (21), it can be seen that acute edema of liver caused by CCl4 intoxication was not a dominant contributor of T1rho increase. After the withdrawal of CCl4 stimulus, it seems Letter to the Editor How liver pathologies contribute to T1rho contrast require more careful studies that the lowering of elevated liver T1rho was faster than the resolve of fiber tissue deposition in rat liver.…”

mentioning

confidence: 73%

How liver pathologies contribute to T1rho contrast require more careful studies

Wáng

Chen

Deng

2017

Quant. Imaging Med. Surg.

Self Cite

View full text Add to dashboard Cite

mentioning

confidence: 73%

How liver pathologies contribute to T1rho contrast require more careful studies

Wáng

Chen

Deng

2017

Quant. Imaging Med. Surg.

Self Cite

View full text Add to dashboard Cite

“…A number of MR imaging techniques have been investigated to assess early liver parenchyma fibrosis, including T 1 rho imaging [26–31], tagged MRI assessing liver strain [32], MR elastography [33, 34] and intravoxel incoherent motion (IVIM) technique [35, 36]. Our current study for the first time evaluated the feasibility of CEST imaging of in vivo liver at the clinical field strength of 3T, demonstrating the difference between over-night-fast and post-meal statuses.…”

Section: Discussionmentioning

confidence: 99%

Chemical Exchange Saturation Transfer (CEST) MR Technique for Liver Imaging at 3.0 Tesla: an Evaluation of Different Offset Number and an After-Meal and Over-Night-Fast Comparison

et al. 2015

Self Cite

View full text Add to dashboard Cite

Purpose To explore whether Chemical Exchange Saturation Transfer (CEST) MRI can detect liver composition changes between after-meal and over-night-fast statuses. Procedures 15 healthy volunteers were scanned on a 3.0 T human MRI scanner in the evening 1.5–2 hour after dinner and in the morning after over-night (12-hour) fasting. Among them seven volunteers were scanned twice to assess the scan-rescan reproducibility. Images were acquired at offsets (n=41, increment=0.25ppm) from −5 to 5ppm using a turbo spin echo (TSE) sequence with a continuous rectangular saturation pulse. Amide proton transfer-weighted (APTw) and GlycoCEST signals were quantified with the asymmetric magnetization transfer ratio (MTRasym) at 3.5ppm and the total MTRasym integrated from 0.5 to 1.5ppm from the corrected Z-spectrum, respectively. To explore scan time reduction, CEST images were reconstructed using 31 offsets (with 20% time reduction) and 21 offsets (with 40% time reduction), respectively. Results For reproducibility, GlycoCEST measurements in 41 offsets showed the smallest scan-rescan mean measurements variability, indicated by lowest mean difference of −0.049% (95% limits of agreement: −0.209% to 0.111%); for APTw, the smallest mean difference was found to be 0.112% (95% limits of agreement: −0.698% to 0.921%) in 41 offsets. Compared with after-meal, both GlycoCEST measurement and APTw measurement under different offset number decreased after 12-hour fasting. However, as the offsets number decreased (41 offsets vs. 31 offsets vs. 21 offsets), GlycoCEST map and APTw map became more heterogeneous and noisier. Conclusion Our results show that CEST liver imaging at 3.0 T has high sensitivity for fasting.

show abstract

“…In this study T2 relaxation's contamination to T1ρ elevation was not investigated, as it is known that rat BDL model is only associated with only mild inflammation in portal spaces (17,18,(26)(27)(28). In addition, previous study on CCl4 induced liver fibrosis model development demonstrated T2 and T1ρ follow different time courses; therefore T2 lengthening will not considerably contribute to T1ρ lengthening in this study (39). Another limitation is that only one liver fibrosis model is used in this study.…”

Section: Bdl Is a Well Established Model For Liver Fibrosis Researchmentioning

confidence: 92%

Black blood T1rho MR imaging may diagnose early stage liver fibrosis: a proof-of-principle study with rat biliary duct ligation model

Koon¹,

Zhang²,

Chen

et al. 2016

Quant. Imaging Med. Surg.

View full text Add to dashboard Cite

Background: To explore black blood T1rho (T1ρ) liver imaging and investigate the earliest stage when biliary duct ligation (BDL) induced liver fibrosis can be diagnosed.Methods: MR was performed at 3 Tesla. A T1ρ prepared 2D fast spin echo (FSE) sequence with acquisition of four spin lock times (TSLs: 1, 10, 30, and 50 msec) and spin-lock frequency of 500 Hz was applied.Inherent black blood effect of FSE and double inversion recovery (DIR) achieved blood signal suppression, and 3 axial sections per liver were obtained. Male Sprague-Dawley rats were scanned at baseline (n=32), and on day-3 (n=13), day-5 (n=11), day-7 (n=10), day-10 (n=4) respectively after BDL. Hematoxylin-eosin (HE) and picrosirius red staining liver histology was obtained at these time points.Results: The physiological liver parenchyma T1ρ was 38.38±1.53 msec (range, 36.05-41.53 msec). Liver T1ρ value elevated progressively after BDL. On day-10 after BDL all experimental animals can be separated from normal liver based on T1ρ measurement with lowest value being 42.82 msec. Day-7 and day-10 liver resembled METAVIR stage-F1/F2 fibrosis, and fibrous area counted for 0.22%±0.13% and 0.38%±0.44% of liver parenchyma area, respectively. Conclusions:This study provides the first proof-of-principle that T1ρ might diagnose early stage liver fibrosis.

show abstract

MR T1ρ as an imaging biomarker for monitoring liver injury progression and regression: an experimental study in rats with carbon tetrachloride intoxication

Abstract: • MR T1ρ is a valuable imaging biomarker for liver injury/fibrosis. • Liver T1ρ was only mildly affected by oedema and acute inflammation. • Liver MR T1ρ decreased when liver fibrosis and injury regressed.

Cited by 53 publications

References 46 publications

How liver pathologies contribute to T1rho contrast require more careful studies

How liver pathologies contribute to T1rho contrast require more careful studies

Chemical Exchange Saturation Transfer (CEST) MR Technique for Liver Imaging at 3.0 Tesla: an Evaluation of Different Offset Number and an After-Meal and Over-Night-Fast Comparison

Black blood T1rho MR imaging may diagnose early stage liver fibrosis: a proof-of-principle study with rat biliary duct ligation model

Contact Info

Product

Resources

About